IL‐10 induces MC3T3‐E1 cells differentiation towards osteoblastic fate in murine model

Xiong, Yuan; Yan, Chenchen; Chen, Lang; Endo, Yori; Sun, Yun; Zhou, Wu; Hu, Yiqiang; Hu, Liangcong; Chen, Dong; Xue, Hang; Mi, Bobin; Liu, Guohui

doi:10.1111/jcmm.14832

Cited by 29 publications

(21 citation statements)

References 41 publications

(83 reference statements)

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“…Moreover, IL-10 is involved in bone remodeling. It has been shown that IL-10 promotes osteogenic differentiation of MC3T3-E1 [36]. The absence of IL-10 could lead to accelerated resorption of alveolar bone [37].…”

Section: Discussionmentioning

confidence: 99%

Human Gingiva-derived Mesenchymal Stem Cells Promote Osteogenic Differentiation via Suppressing T Cell Biological Activity

Zhao

Zhu

et al. 2021

Preprint

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Background: Accumulating evidence has revealed that human gingiva-derived mesenchymal stem cells (GMSCs) are emerging as a new line of mesenchymal stem cells and have a robust immune regulatory function and regenerative ability. However, the relationship between immune regulatory function and regenerative ability is unclear, whether GMSCs promoted osteoblastic differentiation by regulating immune cells. Methods: In this study, we investigated the effect of GMSCs regulating T-cell biological activity on osteoblasts in a direct contact co-culture system. GMSCs and T-cell were co-cultured, the co-culture supernatant was collected and acted on MC3T3-E1 cells, then the alkaline phosphatase (ALP) staining, Alizarin Red staining, Immunofluorescence staining, and real-time quantitative PCR (RT-qPCR) analysis of osteogenic genes were detected to evaluated the osteogenesis differentiation of MC3T3-E1 cells. Results: Our results demonstrated that GMSCs could suppress the activated T-cell function by downregulation pro-inflammatory cytokines (Interleukin-1β [IL-1β] and Tumor necrosis factor-α [TNF-α]) and the upregulation of anti-inflammatory cytokines (IL-10). Meanwhile, the co-culture supernatant significantly increased osteogenic differentiation of ME3T3-E1.Conclusion: GMSCs could promote the osteogenic differentiation of MC3T3-E1 cells by inhibiting the biological activity of activated T-cell.

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Section: Discussionmentioning

confidence: 99%

Human Gingiva-derived Mesenchymal Stem Cells Promote Osteogenic Differentiation via Suppressing T Cell Biological Activity

Zhao

Zhu

et al. 2021

Preprint

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“…In this study, the effect of miR-6979-5p on fracture healing was investigated in vivo, with the results suggesting that miR-6979-5p inhibits fracture healing, and we were able to confirm this negative impact on osteogenic differentiation using MC3T3-E1 cells. As previous studies [30] have reported miRNAs can bind to target mRNAs to exert specific effects, we subsequently explored the downstream targets of miR-6979-5p using luciferase assays.…”

Section: Discussionmentioning

confidence: 99%

The lncRNA Rhno1/miR-6979-5p/BMP2 Axis Modulates Osteoblast Differentiation

Xiong¹,

Chen²,

Yan³

et al. 2020

Int. J. Biol. Sci.

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The roles of long non-coding RNAs (lncRNAs) and micro RNAs (miRNAs) as regulators of mRNA expression in various diseases have recently been reported. Osteoblast differentiation is the vital process which mediates bone formation and fracture healing. In present study, we found microRNA-6979-5p (miR-6979-5p) to be the most differentially expressed miRNA between normal bone and calluses of mice, and overexpression of miR-6979-5p was negatively associated with osteoblast differentiation. Through luciferase assays, we found evidence that bone morphogenetic protein 2 (BMP2) is a miR-6979-5p target gene that positively regulates osteoblast differentiation. We further identified the lncRNA Rhno1 as a competing endogenous RNA (ceRNA) of miR-6979-5p, and we verified that it was able to influence osteoblast differentiation both in vitro and in vivo. In summary, our data indicates that the lncRNA Rhno1/miR-6979-5p/BMP2 axis is a significant regulatory mechanism controlling osteoblast differentiation, and it may thus offer a novel therapeutic strategy for fracture healing.

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“…In Xiong et al, 1 the published article contains errors in Figure 1B and Figure 4F. The correct figures are shown below.…”

Section: Figurementioning

confidence: 97%

Corrigendum

2021

J Cellular Molecular Medi

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In Xiong et al, 1 the published article contains errors in Figure 1B and Figure 4F. The correct figures are shown below. The authors confirm all results and conclusions of this article remain unchanged. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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IL‐10 induces MC3T3‐E1 cells differentiation towards osteoblastic fate in murine model

Cited by 29 publications

References 41 publications

Human Gingiva-derived Mesenchymal Stem Cells Promote Osteogenic Differentiation via Suppressing T Cell Biological Activity

Human Gingiva-derived Mesenchymal Stem Cells Promote Osteogenic Differentiation via Suppressing T Cell Biological Activity

The lncRNA Rhno1/miR-6979-5p/BMP2 Axis Modulates Osteoblast Differentiation

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