The lncRNA Rhno1/miR-6979-5p/BMP2 Axis Modulates Osteoblast Differentiation

Xiong, Yuan; Chen, Lang; Yan, Chenchen; Endo, Yori; Mi, Bobin; Liu, Guohui

doi:10.7150/ijbs.38930

Cited by 20 publications

(19 citation statements)

References 42 publications

(36 reference statements)

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“…Osteoporosis is a chronic metabolic bone disorder observed commonly in aged individuals, and is characterized by low bone mineral density, increased brittleness of bone, damaged bone micro-structure, and significant increase in the number of non-stress fractures [ 12 ]. BN is one of the most abundant flavonoids in Scutellaria baicalensis and is widely used in Chinese herbal medicine to treat various human diseases for centuries.…”

Section: Discussionmentioning

confidence: 99%

Baicalein ameliorates osteoporosis via AKT/FOXO1 signaling

Cai

Yin

et al. 2021

Aging

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In this study, we used bioinformatics and an in vitro cellular model of glucocorticoid-induced osteoporosis to investigate mechanisms underlying the beneficial effects of baicalein (BN) against osteoporosis. STITCH database analysis revealed 30 BN-targeted genes, including AKT1, CCND1, MTOR , and PTEN . Functional enrichment analysis demonstrated that BN-targeted genes were enriched in 49 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. MIRWALK2.0 database analysis identified 110 enriched KEGG pathways related to osteoporosis. A Venn diagram demonstrated that 26 KEGG pathways were common between osteoporosis and BN-targeted genes. The top 5 common KEGG pathways were prostate cancer, bladder cancer, glioma, pathways in cancer, and melanoma. BN-targeted genes in the top 5 shared KEGG pathways were involved in PI3K-AKT, MAPK, p53, ErbB, and mTOR signaling pathways. In addition, glucocorticoid-induced osteoporosis in MC3T3-E1 cells was partially reversed by BN through inhibition of AKT, which, by upregulating FOXO1, enhanced expression of bone turnover markers (ALP, OCN, Runx2, and Col 1) and extracellular matrix mineralization. These findings demonstrate that BN suppresses osteoporosis via an AKT/FOXO1 signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Baicalein ameliorates osteoporosis via AKT/FOXO1 signaling

Cai

Yin

et al. 2021

Aging

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“…Xiong et al [49] studied whether the lncRNA Rhno1/miR-6979-5p/BMP2 axis could be involved in the regulation of osteoblast differentiation. They observed that the expression levels of miRNA-6979-5p are deregulated between the calluses obtained from the femoral fracture mouse model and mouse models with normal bone.…”

Section: Lncrnas As Positive Regulatorsmentioning

confidence: 99%

The Involvement of Long Non-Coding RNAs in Bone

Aurilia

Donati

Palmini

et al. 2021

IJMS

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A harmonious balance between osteoblast and osteoclast activity guarantees optimal bone formation and resorption, pathological conditions affecting the bone may arise. In recent years, emerging evidence has shown that epigenetic mechanisms play an important role during osteoblastogenesis and osteoclastogenesis processes, including long non-coding RNAs (lncRNAs). These molecules are a class of ncRNAs with lengths exceeding 200 nucleotides not translated into protein, that have attracted the attention of the scientific community as potential biomarkers to use for the future development of novel diagnostic and therapeutic approaches for several pathologies, including bone diseases. This review aims to provide an overview of the lncRNAs and their possible molecular mechanisms in the osteoblastogenesis and osteoclastogenesis processes. The deregulation of their expression profiles in common diseases associated with an altered bone turnover is also described. In perspective, lncRNAs could be considered potential innovative molecular biomarkers to help with earlier diagnosis of bone metabolism-related disorders and for the development of new therapeutic strategies.

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“…LncRNAs do not directly encode for proteins. However, an increasing number of studies have rejected their classification as "junk" and confirmed the important biological functions that they play in a variety of biological processes, including genome imprinting [42], cell differentiation [43], apoptosis [44], stem cell pluripotency [45], X chromosome inactivation [46], and nuclear transport [47][48][49]. The biological functions of lncRNAs are described in detail in our previous paper [20], which mainly focused on classification and regulatory mechanisms.…”

Section: The Classification Of Long Noncoding Rnasmentioning

confidence: 99%

Long Noncoding RNAs: Recent Insights into Their Role in Male Infertility and Their Potential as Biomarkers and Therapeutic Targets

Zhao

Heng

Sahlu

et al. 2021

IJMS

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Long noncoding RNAs (lncRNAs) are composed of nucleotides located in the nucleus and cytoplasm; these are transcribed by RNA polymerase II and are greater than 200 nt in length. LncRNAs fulfill important functions in a variety of biological processes, including genome imprinting, cell differentiation, apoptosis, stem cell pluripotency, X chromosome inactivation and nuclear transport. As high throughput sequencing technology develops, a substantial number of lncRNAs have been found to be related to a variety of biological processes, such as development of the testes, maintaining the self-renewal and differentiation of spermatogonial stem cells, and regulating spermatocyte meiosis. These indicate that lncRNAs can be used as biomarkers and potential therapeutic targets for male infertility. However, only a few comprehensive reviews have described the role of lncRNAs in male reproduction. In this paper, we summarize recent findings relating to the role of lncRNAs in spermatogenesis, their potential as biomarkers for male infertility and the relationship between reproductive arrest and transgenerational effects. Finally, we suggest specific targets for the treatment of male infertility from the perspective of lncRNAs.

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The lncRNA Rhno1/miR-6979-5p/BMP2 Axis Modulates Osteoblast Differentiation

Cited by 20 publications

References 42 publications

Baicalein ameliorates osteoporosis via AKT/FOXO1 signaling

Baicalein ameliorates osteoporosis via AKT/FOXO1 signaling

The Involvement of Long Non-Coding RNAs in Bone

Long Noncoding RNAs: Recent Insights into Their Role in Male Infertility and Their Potential as Biomarkers and Therapeutic Targets

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