MicroRNA‐92b‐3p is a prognostic oncomiR that targets TSC1 in clear cell renal cell carcinoma

Abstract

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractAlthough several studies have reported that microRNA (miR)-92b-3p is involved in various cellular processes related to carcinogenesis, its physiological role in clear cell renal cell carcinoma (ccRCC) remains unclear. To clarify the role of miR-92b-3p in ccRCC, we compared miR-92b-3p expression levels in ccRCC tissues and adjacent normal renal tissues. Significant upregulation of miR-92b-3p was observed in ccRCC tissues. Overexpression of miR-92b-3p using a miRNA mimic promoted proliferation, migration, and invasion activities of ACHN cells. Functional inhibition of miR-92b-3p by a hairpin miRNA inhibitor suppressed Caki-2 cell growth and invasion activities in vitro. Mechanistically, it was found that miR-92b-3p directly targeted the TSC1 gene, a known upstream regulator of mTOR. Overexpression of miR-92b-3p decreased the protein expression of TSC1 and enhanced the downstream phosphorylation of p70S6 kinase, suggesting that the mTOR signaling pathway was activated by miR-92b-3p in RCC cells. Importantly, a multivariate Cox proportion hazard model, based on TNM staging and high levels of miR-92b-3p, revealed that miR-92b-3p expression (high vs.low hazard ratio, 2.86; 95% confidence interval, 1.20-6.83; P = .018) was a significant prognostic factor for overall survival of ccRCC patients with surgical management.Taken together, miR-92b-3p was found to act as an oncomiR, promoting cell proliferation by downregulating TSC1 in ccRCC. K E Y W O R D SccRCC, miR-92b-3p, oncomiR, proliferation, TSC1

MiR-92b-3p Inhibits Proliferation of HER2-Positive Breast Cancer Cell by Targeting circCDYL

“…The role of miR-92b-3p in cancer is contentious. MiR-92b-3p exhibited tumor-promoting role in proliferation, migration and invasion of colorectal, renal, gastric and prostate cancer cells ( Gong et al, 2018 ; Li C. et al, 2019 ; Wang et al, 2020 ; Wang G. et al, 2021 ). On the contrary, miR-92b-3p suppresses tumor progression of pancreatic cancer by targeting Gabra3 ( Long et al, 2017 ).…”

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“…miR-92b-3p has been reported as a suppressor due to down-regulation www.nature.com/scientificreports/ in several cancers 30 . However, recent studies have also proposed that miR-92b-3p plays an oncogenic role for proliferation, migration, and invasion of a few cancers, such as glioblastoma 31 , colorectal cancer 32 , renal cell carcinoma 33 , and gastric cancer 34 . Exosomal miR-92b-3p has also been considered as a potential dynamic biomarker to monitor chemoresistance in small-cell lung cancer 35 .and synovial sarcoma 36 .…”

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“…miR-92b-3p has been reported as a suppressor due to down-regulation in several cancers 29 . However, recent studies have also proposed that miR-92b-3p plays an oncogenic role for proliferation, migration, and invasion of a few cancers, such as glioblastoma 30 , colorectal cancer 31 , renal cell carcinoma 32 , and gastric cancer 33 . Exosomal miR-92b-3p has also been considered as a potential dynamic biomarker to monitor chemoresistance in small-cell lung cancer 34 .and synovial sarcoma 35 .…”