MicroRNA‑654‑3p enhances cisplatin sensitivity by targeting QPRT and inhibiting the PI3K/AKT signaling pathway in ovarian cancer cells

Niu, Yichao; Tong, Jing; Shi, Xiaofei; Zhang, Ting

doi:10.3892/etm.2020.8878

Cited by 21 publications

(20 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…miR-654-3p is downregulated in ovarian cancer ( 47 ), gastric cancer ( 48 ) and papillary thyroid cancer ( 49 ), but is upregulated in osteosarcoma ( 50 ). Moreover, miR-654-3p exerts cancer-inhibiting ( 47 – 49 ) or cancer-promoting roles ( 50 ), and is implicated in cancer oncogenesis and progression ( 47 – 50 ). A recent study reported that miR-654-3p expression was down-regulated in HCC, which was associated with poor patient prognosis, and that overexpression of miR-654-3p restricted cell proliferation, migration and invasion in HCC ( 51 ).…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA MKLN1‑AS aggravates hepatocellular carcinoma progression by functioning as a molecular sponge for miR‑654‑3p, thereby promoting hepatoma‑derived growth factor expression

Gao¹,

Chen²,

Chi³

et al. 2020

Int J Mol Med

View full text Add to dashboard Cite

Long non-coding RNAs (lncRNAs) have recently gained attention due to their important roles in human cancer types, such as breast and gastric cancer. The present study measured alterations in muskelin 1 antisense RNA (MKLN1-AS) expression in hepatocellular carcinoma (Hcc) and evaluated its clinical value in patients with Hcc. Additionally, the current study investigated the effects of MKLN1-AS on the malignant features of Hcc cells. The detailed molecular mechanisms underlying the cancer-promoting activities of MKLN1-AS in Hcc cells were also elucidated. MKLN1-AS expression in Hcc tissues and cell lines was detected using reverse-transcription quantitative PCR (RT-qPCR). Cell Counting Kit-8 assays and flow cytometry were used to determine the roles of MKLN1-AS in Hcc cell proliferation and apoptosis. Migration and invasion assays, as well as tumor xenograft experiments were conducted to analyze migration and invasion in vitro and tumor growth in vivo, respectively. The interaction among microRNA-654-3p (miR-654-3p), MKLN1-AS and hepatoma-derived growth factor (HdGF) in Hcc was investigated using luciferase reporter assay, RNA immunoprecipitation assay, RT-qPcR, western blotting and rescue experiments. MKLN1-AS was upregulated in Hcc tissues and cell lines, and a high MKLN1-AS expression was associated with shorter overall survival and disease-free survival in patients with Hcc. Functionally, the knockdown of MKLN1-AS impaired Hcc cell proliferation, migration and invasion, as well as induced cell apoptosis in vitro. Knockdown of MKLN1-AS expression also inhibited cell proliferation in vivo. The results indicated that MKLN1-AS functioned as a competing endogenous RNA by sponging miR-654-3p in Hcc cells. Additionally, miR-654-3p targeting of HdGF was positively modulated by MKLN1-AS, and miR-654-3p knockdown partially abrogated this effect. Rescue experiments demonstrated that knockdown of miR-654-3p and overexpression of HdGF both abolished MKLN1-AS knockdown-induced cellular processes in Hcc. In summary, MKLN1-AS induced pro-oncogenic effects during Hcc progression by serving as a molecular sponge for miR-654-3p to increase HdGF expression. Therefore, the MKLN1-AS/miR-654-3p/HdGF axis may offer a novel target for the diagnosis, prognosis, prevention and treatment of Hcc.

show abstract

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA MKLN1‑AS aggravates hepatocellular carcinoma progression by functioning as a molecular sponge for miR‑654‑3p, thereby promoting hepatoma‑derived growth factor expression

Gao¹,

Chen²,

Chi³

et al. 2020

Int J Mol Med

View full text Add to dashboard Cite

show abstract

“…MicroRNAs (miRNAs/miRs) are non-coding RNAs that are ~22 nt in length and play key regulatory roles in the development and chemoresistance of human cancers (14). For example, Niu et al (15) revealed that miRNA-654-3p enhanced cisplatin (DDP) sensitivity by downregulating quinolinate phosphoribosyl transferase and suppressing the PI3K/AKT pathway in ovarian cancer. Yang et al (16) suggested that miRNA-181a-5p promoted the sensitivity of esophageal adenocarcinoma cells to DDP by targeting Cbl proto-oncogene B.…”

Section: Introductionmentioning

confidence: 99%

Long non‑coding RNA LINC00173 enhances cisplatin resistance in hepatocellular carcinoma via the microRNA‑641/RAB14 axis

et al. 2021

View full text Add to dashboard Cite

A growing body of evidence indicates that long non-coding RNAs (lncRNAs) play crucial roles in the chemoresistance of human cancers. However, the molecular mechanisms underlying the functions of certain lncRNAs in the chemotherapeutic resistance of hepatocellular carcinoma (HCC) remain unclear. The aim of the present study was to investigate the function and potential mechanism of action of lncRNA LINC00173 in HCC cisplatin (DDP) resistance. Reverse transcription-quantitative PCR analysis indicated that LINC00173 was highly expressed in DDP-resistant HCC tissues and cell lines, and high expression levels of LINC00173 were found to be associated with poor prognosis in patients with HCC. Moreover, LINC00173-knockdown improved the DDP sensitivity of DDP-resistant HCC cells. A luciferase reporter assay also demonstrated that microRNA (miR)-641 was a direct target of LINC00173. miR-641 inhibition restored the promoting effect of LINC00173 knockdown on DDP sensitivity in HCC cells. Furthermore, RAB14 was identified as a target of miR-641, and RAB14 overexpression restrained the inducing effect of LINC00173 knockdown on HCC cell DDP sensitivity. The findings of the present study demonstrated that LINC00173 increased DDP resistance in HCC via the miR-641/RAB14 axis, which may represent a promising therapeutic strategy for HCC.

show abstract

“…Acquired cisplatin resistance is a serious problem for the therapy of OC patients ( 8 , 24 ). miRNAs actually participated in the regulation of tumorigenesis and drug resistance in OC ( 11 , 25 ). By quantified the expression of miR-4461 in cisplatin-resistant cell lines, we found that miR-4461 expression was enhanced in resistant cells, suggesting that high expression of miR-4461 might be associated with cisplatin resistance in OC.…”

Section: Discussionmentioning

confidence: 99%

“…Crucially, increasingly evidence has emerged to emphasize that the function of miRNAs in cisplatin resistance in human cancers. For instance, the sensitivity on the cisplatin of miR-654-3p is enhanced by targeting QPRT and the PI3K/AKT signaling pathway in OC cells is suppressed ( 11 ). MiR-186 achieved the regulation of cisplatin sensitivity on ovarian cancer cells in two directions simultaneously, which not only inhibiting PIK3R3 and PTEN, but also promoting expression of APAF1 as well ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

miR-4461 Regulates the Proliferation and Metastasis of Ovarian Cancer Cells and Cisplatin Resistance

Dou

Zhang

2021

Front. Oncol.

View full text Add to dashboard Cite

microRNAs (miRNAs) are of great significance in cancer treatment, which may have a desirable result on the regulation of tumorigenesis, progression, recurrence, and chemo-resistance of ovarian cancer. However, the research on the further potential application of miR-4461 in ovarian cancer is little and limited. Therefore, the study in this paper focus on the investigation of the of miR-4461 in ovarian cancer progression and chemo-resistance. The phenomenon that the proliferation and metastasis of ovarian cancer cells can be promoted by miR4461 is revealed in functional assays. Through the bioinformatics and luciferase reporter analysis, the PTEN is validated to be the direct target of miR-4461 in ovarian. The association between the expression of miR-4461 and PTEN is negative in in human ovarian cancer tissues. The distinction of growth and metastasis capacity between miR-4461 knockdown ovarian cancer cells and control cells is partially abolished by si-PTEN. Moreover, it was found that cisplatin treatment has obvious effect on the miR-4461 knockdown ovarian cancer cells. In summary, the data given in this paper indicate that the miR-4461 can be regarded as a potential onco-miRNA in ovarian cancer by targeting PTEN.

show abstract

MicroRNA‑654‑3p enhances cisplatin sensitivity by targeting QPRT and inhibiting the PI3K/AKT signaling pathway in ovarian cancer cells

Cited by 21 publications

References 43 publications

Long non‑coding RNA MKLN1‑AS aggravates hepatocellular carcinoma progression by functioning as a molecular sponge for miR‑654‑3p, thereby promoting hepatoma‑derived growth factor expression

Long non‑coding RNA MKLN1‑AS aggravates hepatocellular carcinoma progression by functioning as a molecular sponge for miR‑654‑3p, thereby promoting hepatoma‑derived growth factor expression

Long non‑coding RNA LINC00173 enhances cisplatin resistance in hepatocellular carcinoma via the microRNA‑641/RAB14 axis

miR-4461 Regulates the Proliferation and Metastasis of Ovarian Cancer Cells and Cisplatin Resistance

Contact Info

Product

Resources

About