MicroRNA-615-5p targets insulin-like growth factor 2 and exerts tumor-suppressing functions in human esophageal squamous cell carcinoma

Yang, Bingyin; Xie, Rui; Wu, Shang-Nong; Gao, Chunfang; Yang, Xiaozhong; Zhou, Jing-Fang

doi:10.3892/or.2017.6079

Cited by 13 publications

(11 citation statements)

References 25 publications

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“…However, downregulation of miR-615 was found in non-small cell lung cancer and renal cell carcinoma (11,12). In addition, miR-615 was found to play an inhibitory role in esophageal squamous cell carcinoma by targeting IGF2 (13). These findings indicate that miR-615 has a tissue-specific function in different types of cancer.…”

Section: Introductionmentioning

confidence: 93%

MicroRNA‑615 functions as a tumor suppressor in osteosarcoma through the suppression of HK2

Wang

Zhang

et al. 2020

Oncol Lett

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At present, the regulatory mechanisms of various microRNAs (miRNAs/miRs) have been elucidated in human cancers including osteosarcoma (OS). This study mainly focused on the role of miR-615 in OS, which has not yet been reported. Ninety-two OS tissues and normal samples were used in this study. Human osteoblast hFOB1.19 cells and OS cell line HOS were utilized to detect the expression of miR-615. The expression of miR-615 and gene expression were assessed by RT-qPCR and western blot analysis. Transwell, MTT and luciferase reporter assays were used to investigate the regulatory mechanism of miR-615 in OS. The results revealed that miR-615 expression was reduced in OS tissues and cells, and was associated with poor clinical outcomes and prognosis in OS patients. In addition, overexpression of miR-615 restrained cell viability and metastasis in OS. Furthermore, hexokinase 2 (HK2) was confirmed as a direct target of miR-615. Upregulation of HK2 was detected in OS tissues. The upregulation of HK2 weakened the tumor-suppressive effect of miR-615 in OS. Moreover, miR-615 blocked epithelial-mesenchymal transition (EMT) and inactivated the PI3K/AKT pathway in OS. To conclude, miR-615 acts as a tumor suppressor in OS, thus miR-615 can be used as a target for OS treatment.

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Section: Introductionmentioning

confidence: 93%

MicroRNA‑615 functions as a tumor suppressor in osteosarcoma through the suppression of HK2

Wang

Zhang

et al. 2020

Oncol Lett

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“…Li et al10 reported that miR-223 was upregulated by MYOD and inhibited myoblast proliferation by repressing IGF2. Yang et al11 reported that miR-615-5p targeted IGF2 and functioned as a tumor suppressor in esophageal squamous cell carcinoma (ESCC).…”

Section: Introductionmentioning

confidence: 99%

<p>miR-491-5p functions as a tumor suppressor by targeting IGF2 in colorectal cancer</p>

Lü

Cai

Peng

et al. 2019

CMAR

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Background Dysregulation of miRNAs is critically implicated in tumorigenesis, and aberrant expression of miR-491-5p has been reported to play a key role in initiation and progression of various cancers. However, the biological function and underlying mechanism of miR-491-5p in colorectal cancer (CRC) remain elusive. Methods Quantitative real-time PCR (qRT-PCR) was employed to evaluate the levels of miR-491-5p and IGF2 mRNA expression in CRC tissues, cell lines and plasma. Cell counting kit-8 and colony formation assays were used to detect the effects of miR-491-5p on CRC cell growth. Luciferase reporter assays were applied to confirm the miR-491-5p target gene. In vivo experiments were conducted in nude mice. Results miR-491-5p was found to be obviously downregulated in CRC tissues and cell lines, and decreased miR-491-5p expression level was shown to be associated with differentiation, TNM stage and poor overall survival (OS). miR-491-5p overexpression suppressed CRC cell proliferation both in vitro and in vivo. Mechanically, insulin-like growth factor 2 (IGF2) was identified to be a direct target of miR-491-5p in CRC cells, and overexpression of IGF2 rescued the miR-491-5p-induced suppression of proliferation in CRC cells. Finally, we demonstrated that plasma miR-491-5p expression was decreased in CRC when compared to healthy controls and might be an effective diagnostic biomarker for CRC. Conclusion These data showed that miR-491-5p functioned as a tumor suppressor by targeting IGF2 in CRC, and miR-491-5p could serve as a potential diagnostic and prognostic biomarker for CRC.

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“…Numerous studies have demonstrated that that a number of miRNAs may be aberrantly expressed in ESCC (32-34). Changes in miRNA expression may be associated with ESCC formation and progression, and involved in the regulation of various pathophysiological processes (35)(36)(37). Therefore, identification of ESCC-related miRNAs may help elucidate the mechanisms underlying the pathogenesis of ESCC, which may be useful for the development of improved therapeutic approaches for treating patients with ESCC.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑652 inhibits the biological characteristics of esophageal squamous cell carcinoma by directly targeting fibroblast growth factor receptor 1

Cheng

Huang

2019

Exp Ther Med

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Numerous studies have demonstrated that microRNAs (miRNAs) are dysregulated in esophageal squamous cell carcinoma (ESCC). Changes in miRNA expression may be associated with ESCC formation and progression. Therefore, the identification of ESCC-associated miRNAs may facilitate the development of effective therapeutic approaches for patients with ESCC. Recently, miRNA-652 (miR-652) was recognized as a cancer-associated miRNA in a number of different types of cancer. However, the expression status and roles of miR-652 in ESCC as well as the molecular mechanisms modulated or altered by it remain largely unknown. In the present study, it was demonstrated that miR-652 was downregulated in ESCC tissues and cell lines. Functional assays showed that upregulation of miR-652 expression decreased proliferation and invasion of ESCC cells. Mechanistically, fibroblast growth factor receptor 1 (FGFR1) was determined to be a direct target of miR-652 in ESCC cells. Additionally, FGFR1 was upregulated in ESCC tissues, and the expression of FGFR1 was inversely correlated with miR-652 expression. Furthermore, restoring FGFR1 expression abolished the suppressive effects of miR-652 overexpression on the proliferation and invasion of ESCC cells. These findings demonstrated that miR-652 inhibits the proliferation and invasion of ESCC cells by directly targeting FGFR1.

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MicroRNA-615-5p targets insulin-like growth factor 2 and exerts tumor-suppressing functions in human esophageal squamous cell carcinoma

Cited by 13 publications

References 25 publications

MicroRNA‑615 functions as a tumor suppressor in osteosarcoma through the suppression of HK2

MicroRNA‑615 functions as a tumor suppressor in osteosarcoma through the suppression of HK2

<p>miR-491-5p functions as a tumor suppressor by targeting IGF2 in colorectal cancer</p>

MicroRNA‑652 inhibits the biological characteristics of esophageal squamous cell carcinoma by directly targeting fibroblast growth factor receptor 1

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