MicroRNA-585 suppresses tumor proliferation and migration in gastric cancer by directly targeting MAPK1

Hu, Lei; Wu, Hongmei; Wan, Xiao; Liu, Liu; He, Yi‐Ren; Zhu, Liang; Liu, Shaojun; Yao, Hanhui; Zhu, Zhiqiang

doi:10.1016/j.bbrc.2018.03.116

Cited by 36 publications

(40 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study indicated the potential anti-cancer role of LINC00483 knockdown in gastric cancer, which might be important target for treatment of gastric cancer. MAPK1 has been reported as an important oncogene in gastric cancer progression to promote cell proliferation, migration and invasion [22,23,33,34]. In this research, we also found high expression of MAPK1 in gastric cancer and its knockdown inhibited gastric cancer cell viability, migration and invasion but promoted apoptosis, indicating the carcinogenic role of MAPK1 in gastric cancer.…”

Section: Discussionsupporting

confidence: 65%

LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p

Luo

Liang

2020

Biol Res

View full text Add to dashboard Cite

Background: Previous studies have shown that long noncoding RNA (lncRNA) LINC00483 was aberrantly expressed in human cancers, including gastric cancer. However, the regulatory mechanism of this lncRNA in gastric cancer remains largely unknown. The present study aimed to investigate the effect of LINC00483 on gastric cancer development and explore the potential regulatory network of LINC00483/microRNA (miR)-490-3p/mitogen-activated protein kinase 1 (MAPK1). Methods:Thirty patients with gastric cancer were recruited for tissues collection. The expression levels of LINC00483, miR-490-3p and MAPK1 were detected by quantitative real-time polymerase chain reaction or western blot. Cell viability, apoptosis, migration and invasion were determined by MTT, flow cytometry, transwell assays and western blot, respectively. The target association between miR-490-3p and LINC00483 or MAPK1 was confirmed by luciferase reporter assay. Xenograft model was established to assess the function of LINC00483 in vivo.Results: LINC00483 and MAPK1 levels were increased in gastric cancer tissues and cells. Knockdown of LINC00483 or MAPK1 inhibited cells viability, migration and invasion but promoted apoptosis in gastric cancer cells. Moreover, MAPK1 overexpression attenuated the effect of LINC00483 knockdown on gastric cancer development. LINC00483 could increase MAPK1 expression by competitively sponging miR-490-3p. miR-490-3p overexpression suppressed gastric cancer development, which was abated by introduction of LINC00483. Besides, inhibition of LINC00483 decreased xenograft tumor growth by regulating miR-490-3p/MAPK1 axis. Conclusion:Knockdown of LINC00483 inhibited gastric cancer development in vitro and in vivo by increasing miR-490-3p and decreasing MAPK1, elucidating a novel mechanism for understanding the development of gastric cancer.

show abstract

Section: Discussionsupporting

confidence: 65%

LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p

Luo

Liang

2020

Biol Res

View full text Add to dashboard Cite

show abstract

“…[16] Multiple miRNAs were dysregulated in gastric cancer and played an important role in promoting or inhibiting carcinogenesis and metastasis of gastric cancer, such as miR-338, miR-585 and miR-491-5p. [17][18][19] In the present study, we observed a significant decrease in miR-324-5p expression in human gastric carcinoma tissues, and overexpressed miR-324-5p obviously inhibited the survival of gastric carcinoma cells in vitro. Our findings indicated that miR-324-5p acted as a tumour suppressor gene in gastric cancer.…”

Section: Discussionmentioning

confidence: 63%

“…MiRNAs, widely expressed in eukaryotic organisms, were implicated in the development of tissues and organs, cell proliferation, differentiation, apoptosis, lipid metabolism, hormone secretion and so on, and have been confirmed to involve in the pathogenesis of human cancers . Multiple miRNAs were dysregulated in gastric cancer and played an important role in promoting or inhibiting carcinogenesis and metastasis of gastric cancer, such as miR‐338, miR‐585 and miR‐491‐5p …”

Section: Discussionmentioning

confidence: 99%

MiR-324-5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8

Lin

Zhou

Zhang

et al. 2018

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

show abstract

“…Wang et al showed that MAPK1 upregulation was useful for tumor growth promotion in thyroid cancer cells [22]. Importantly, previous studies indicated that miR-585 could suppress the proliferation and migration by targeting MAPK1 in gastric cancer [38]. Besides, ERBB4 was found to be reduced in PTC cells [39].…”

Section: Discussionmentioning

confidence: 99%

miR-326 inhibits the progression of papillary thyroid carcinoma by targeting MAPK1 and ERBB4

Nie

Wei

et al. 2020

neo

View full text Add to dashboard Cite

Papillary thyroid carcinoma (PTC) is the prevalent histotype of thyroid cancer, with increasing incidence worldwide. MicroRNAs (miRNAs) could play an important role in the development and progression of human cancers. Interestingly, miR-326 was validated as one of the downregulated miRNAs in PTC. Therefore, it is necessary to research the function of miR-326 involved in the progression of PTC. In the current study, we detected the downregulation of miR-326 in PTC tissues and cell lines. The miR-326 overexpression or knockdown was conducted in TPC-1 or HTh83 PTC cells. miR-326 mimics decreased the proliferation, clone formation ability and caused G1-phase accumulation. In addition, the reduction of migration and invasion abilities was induced by miR-326 mimics. Western blot analysis showed that the cells with miR-326 mimics exhibited the inhibition of vimentin and N-cadherin, as well as enhancement of E-cadherin. Importantly, miR-326 could directly target mitogen activated protein kinase 1 (MAPK1) and epidermal growth factor receptor 4 (ERBB4). MAPK1 or ERBB4 overexpression rescued the effects of miR-326 on proliferation, migration, and invasion in PTC cells. Notably, miR-326 reduced tumorigenesis in vivo, including the decrease of tumor volume and weight, suppression of Ki-67, N-cadherin, MAPK1 and ERBB4. In all, these results might provide a new therapeutic target for the diagnosis of PTC.

show abstract

MicroRNA-585 suppresses tumor proliferation and migration in gastric cancer by directly targeting MAPK1

Cited by 36 publications

References 20 publications

LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p

LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p

MiR-324-5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8

miR-326 inhibits the progression of papillary thyroid carcinoma by targeting MAPK1 and ERBB4

Contact Info

Product

Resources

About