MicroRNA-584 and the Protein Phosphatase and Actin Regulator 1 (PHACTR1), a New Signaling Route through Which Transforming Growth Factor-β Mediates the Migration and Actin Dynamics of Breast Cancer Cells

Fils-Aimé, Nadège; Dai, Meiou; Guo, Jimin; El-Mousawi, Mayada; Kahramangil, Bora; Neel, Jean-Charles; Lebrun, Jean-Jacques

doi:10.1074/jbc.m112.430934

Cited by 69 publications

(73 citation statements)

References 78 publications

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“…Guled et al [10] reported that the expression level of miR-584 in malignant mesothelioma was higher compared with normal mesothelium, but a functional analysis was not performed in that report. Regulation of miR-584 and regulation of its novel target PHACTR1 were reported as the necessary steps for breast cancer cell migration [1]. And in 2011, a study revealed that miR-584 acts as tumor suppressor by directly targeting the oncogene Rock-1 and decreasing the invasion ability of human clear-cell renal cell carcinoma [6]; this was the first report to show the tumor suppressor functions of miR-584 and the related mechanism.…”

Section: Discussionmentioning

confidence: 99%

“…But little is known on the role of miRNAs in suppressing invasion and migration of TC, let alone their functions and mechanisms of action. It has been reported that the expression of miR-584 can be up-regulated in breast cancer [1], renal clear-cell carcinoma [6], colon cancer [7], rectal cancer [8], myeloid leukemia [9,] and malignant mesothelioma [10,] to suppress the invasion and migration of tumors by targeting PHACTR1 [1], Rock-1 [6], NGX6 [7], etc. Uncovering the target gene of miR-584 to control TC invasion and migration is of central importance in the diagnosis, treatment, and prognosis of TC, and for blocking tumor invasion and migration.…”

Section: Introductionmentioning

confidence: 99%

“…Papillary TC (PTC) is the class of tumor with the highest morbidity, comprising over 80% of the TCs [1]. The tumor cells in patients with clinically advanced PTC often invade the throat, the trachea, the epiglottis, the esophagus, cervical vessels, and the surrounding structures; they even metastasize to the bone and can cause lung, brain, and systemic metastases.…”

Section: Introductionmentioning

confidence: 99%

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miR-584 Suppresses Invasion and Cell Migration of Thyroid Carcinoma by Regulating the Target Oncogene ROCK1

Xiang

et al. 2015

Oncol Res Treat

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Background: Uncovering the target gene of miR-584 to control thyroid carcinoma (TC) invasion and migration is of central importance in the diagnosis, treatment, and prognosis of TC. To validate whether miR-584 has a tumor-suppressive role in thyroid cancer cells by targeting ROCK1, a series of experiments were conducted to figure out the mechanism of action of miR-584. Material and Methods: Migration analyses and cell proliferation assays were performed using miR-584-transfected cells. The expression levels of miR-584 in TC were detected by using real-time polymerase chain reaction (PCR). Western blot analyses were conducted to find out the relationship between the tumor suppressor miR-584 and the target oncogene ROCK1 protein expression levels. Wound healing experiments were used to examine the relationships between miR-584 and the migration of thyroid cancer K1 cells and the effects of ROCK1 knockdown on K1 cell motility. Results: Our results demonstrate that altering the miR-584 levels affects human thyroid cancer cell migration, but has no effect on cell proliferation. The relative ROCK-1 expression levels were 1 and 0.54 in the scrambled-sequence control group and the miR-584 group, respectively. K1 cells transfected with siRNA-ROCK-1 showed weaker cell migration than cells transfected with siRNA-NC (negative control); the cell motility ratios were 18% and 27%, respectively. Conclusion: These results indicate that miR-584 could inhibit the expression of ROCK1, and ROCK1 knockdown would further affect the migration ability of K1 cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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miR-584 Suppresses Invasion and Cell Migration of Thyroid Carcinoma by Regulating the Target Oncogene ROCK1

Xiang

et al. 2015

Oncol Res Treat

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“…Recent studies have shown the participation of miRNAs in breast cancer metastasis (Harquail et al, 2012). These studies have shown, for example, that miRlet-7, miR-584, and miR-204 inhibits cell motility by regulating the genes in the actin cytoskeleton pathway, implicating a central role for it in cytoskeletal dynamics (Imam et al, 2012;Fils-Aimé et al, 2013;Hu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

miR-485 Acts as a Tumor Suppressor by Inhibiting Cell Growth and Migration in Breast Carcinoma T47D Cells

Anaya-Ruı́z

Bandala²,

Pérez-Santos³

2013

Asian Pacific Journal of Cancer Prevention

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MicroRNAs (miRNAs) are small, non-coding RNAs (18-25 nucleotides) that post-transcriptionally modulate gene expression by negatively regulating the stability or translational efficiency of their target mRNAs. In this context, the present study aimed to evaluate the in vitro effects of miR-485 mimics in breast carcinoma T47D cells. Forty-eight hours after T47D cells were transfected with miR-485 mimics, an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was utilized to determine the effects on cell viability. Colony formation and cell migration assays were adopted to determine whether miR-485 affects the proliferation rates and cell migration of breast carcinoma T47D cells. Our results showed that ectopic expression of miR-485 resulted in a significant decrease in cell growth, cell colony formation, and cell migration. These findings suggest that miR-485 might play an important role in breast cancer by suppressing cell proliferation and migration.

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“…Although the precise function of PHACTR1 remains unclear, associations with cell migration, motility and invasiveness in tumor tissues have been reported (24). Table III.…”

Section: Discussionmentioning

confidence: 99%

Association of a transcription factor 21 gene polymorphism with hypertension

Fujimaki

Oguri²,

Horibe

et al. 2014

Biomedical Reports

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Abstract.Various loci and genes that confer susceptibility to coronary artery disease (CAD) have been identified mainly in Caucasian populations by genome-wide association studies (GWASs). As hypertension is a major risk factor for CAD, certain polymorphisms may contribute to the genetic susceptibility to CAD through affecting the predisposition to hypertension. The aim of the present study was to examine a possible association of hypertension with 29 single-nucleotide polymorphisms (SNPs) previously identified by meta-analyses of GWASs as susceptibility loci for CAD. Study subjects comprised of 5,460 individuals (3,348 subjects with hypertension and 2,112 controls). The genotypes of SNPs were determined by the multiplex bead-based Luminex assay. The χ 2 test revealed that genotype distributions and allele frequencies for rs12190287 of the transcription factor 21 gene (TCF21) and rs1122608 of the SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily a, member 4 gene (SMARCA4) were significantly (P<0.05) associated with hypertension. Allele frequencies for rs9369640 of the phosphatase and actin regulator 1 gene (PHACTR1) and genotype distributions for rs599839 of the proline/serine-rich coiled-coil 1 gene (PSRC1) were also significantly associated with hypertension. Multivariable logistic regression analysis with adjustment for age, gender, body mass index and smoking status revealed that rs12190287 of TCF21 (P=0.0014; recessive model; odds ratio, 1.21) was significantly associated with hypertension, and the C allele represented a risk factor for this condition. Similar analyses revealed that rs1122608 of SMARCA4 (P=0.0305; dominant model; odds ratio, 0.86), rs9369640 of PHACTR1 (P= 0.0119; dominant model; odds ratio, 0.82) and rs599839 of PSRC1 (P=0.0248; dominant model; odds ratio, 0.84) were also related to hypertension, with the minor T, C and G alleles, respectively, being protective against this condition. Thus, the present results indicate that rs12190287 (G→C) of TCF21 is a susceptibility locus for hypertension.

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MicroRNA-584 and the Protein Phosphatase and Actin Regulator 1 (PHACTR1), a New Signaling Route through Which Transforming Growth Factor-β Mediates the Migration and Actin Dynamics of Breast Cancer Cells

Cited by 69 publications

References 78 publications

miR-584 Suppresses Invasion and Cell Migration of Thyroid Carcinoma by Regulating the Target Oncogene <b><i>ROCK1</i></b>

miR-584 Suppresses Invasion and Cell Migration of Thyroid Carcinoma by Regulating the Target Oncogene <b><i>ROCK1</i></b>

miR-485 Acts as a Tumor Suppressor by Inhibiting Cell Growth and Migration in Breast Carcinoma T47D Cells

Association of a transcription factor 21 gene polymorphism with hypertension

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