miR-584 Suppresses Invasion and Cell Migration of Thyroid Carcinoma by Regulating the Target Oncogene <b><i>ROCK1</i></b>

Abstract

Background: Uncovering the target gene of miR-584 to control thyroid carcinoma (TC) invasion and migration is of central importance in the diagnosis, treatment, and prognosis of TC. To validate whether miR-584 has a tumor-suppressive role in thyroid cancer cells by targeting ROCK1, a series of experiments were conducted to figure out the mechanism of action of miR-584. Material and Methods: Migration analyses and cell proliferation assays were performed using miR-584-transfected cells. The expression levels of miR-584 in TC were detected by using real-time polymerase chain reaction (PCR). Western blot analyses were conducted to find out the relationship between the tumor suppressor miR-584 and the target oncogene ROCK1 protein expression levels. Wound healing experiments were used to examine the relationships between miR-584 and the migration of thyroid cancer K1 cells and the effects of ROCK1 knockdown on K1 cell motility. Results: Our results demonstrate that altering the miR-584 levels affects human thyroid cancer cell migration, but has no effect on cell proliferation. The relative ROCK-1 expression levels were 1 and 0.54 in the scrambled-sequence control group and the miR-584 group, respectively. K1 cells transfected with siRNA-ROCK-1 showed weaker cell migration than cells transfected with siRNA-NC (negative control); the cell motility ratios were 18% and 27%, respectively. Conclusion: These results indicate that miR-584 could inhibit the expression of ROCK1, and ROCK1 knockdown would further affect the migration ability of K1 cells.

MicroRNA-584 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting MTDH

“…Additionally, miR-584 is an independent prognostic factor for the outcome in neuroblastoma patients (38). Downregulation of miR-584 was also observed in clear cell renal cell carcinoma (21), thyroid carcinoma (22,23) and glioma (24). These findings suggested that aberrant expression of miR-584 is a potential prognostic factor in these cancer types.…”

“…Ueno et al revealed that miR-584 overexpression decreased cell motility in clear cell renal cell carcinoma (21). Moreover, Orlandella et al showed that resumed expression of miR-584 inhibited the migration and invasion of thyroid carcinoma cells and induced their resistance to apoptosis (22,23). Wang et al indicated that miR-584 reexpression repressed cell groWTh, migration, invasion and vasculogenic mimicry of glioma (24,39,40).…”

“…Multiple targets of miR-584 have been validated, including WWP1 (37) in gastric cancer, MMP14 (38) in neuroblastoma, ROCK1 (21) in clear cell renal cell carcinoma, TUSC2 (23) and ROCK1 (22) in thyroid carcinoma, and PTTG1IP (24) and ROCK1 (39,40) in glioma. These studies suggest that miR-584 exhibits tissue-specific functions.…”

“…MiR-584 functions as tumour suppressor in several types of cancer (21)(22)(23)(24). In NSCLC, miR-584 expression was reported to be downregulated in tissue samples (25) and upregulated in the plasma of NSCLC patients (26).…”

Increased Expression of miR-29a and Its Prognostic Significance in Patients with Cholangiocarcinoma

“…It is easy to collect miRNAs from serum samples due to their stable form which can resist RNase digestion and other harsh conditions [13,14]. Moreover, miRNAs have been reported to regulate gene expression at the post-transcriptional level by binding to the 3'-untranslated region (3'-UTR) of targeted mRNAs [15], which might contribute their potential role in the progression of human cancers including CCA [16][17][18][19]. For example, Wang et al [20] suggested that microRNA-26a might play a crucial role in the diagnosis and prognosis of CCA, and provide a novel therapeutic target for this malignancy.…”