MicroRNA-532-3p Regulates Pro-Inflammatory Human THP-1 Macrophages by Targeting ASK1/p38 MAPK Pathway

Dinesh, Palani; Kalaiselvan, Sowmiya; Sujitha, Sali; Rasool, Mahaboobkhan

doi:10.1007/s10753-020-01325-7

Cited by 9 publications

(10 citation statements)

References 43 publications

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“…hsa‐miR‐532‐3p diminished the levels of ASK1 and downstream phosphorylation/translocation of p38 MAPK during lipopolysaccharide (LPS)/ tumor necrosis factor‐α (TNF‐α)‐induced inflammation in macrophages and reduced the release of various pro‐inflammatory cytokines and chemokines, including IL‐6 and TNF‐alpha. 9 IL‐6 is a proinflammatory cytokine that has been reported to be involved in the cytokine storm observed in severe COVID‐19. 10 The down‐regulation of anti‐inflammatory miRNAs, such as hsa‐miR‐532‐3p, hsa‐miR‐340‐5p and hsa‐miR‐455‐5p, in severe COVID‐19 (Table 1 ) is in line with a hyperinflammatory state in severe COVID‐19.…”

Section: Figurementioning

confidence: 99%

Altered microRNA expression in severe COVID‐19: Potential prognostic and pathophysiological role

Garnier

Pollet²,

Fourcot³

et al. 2022

Clinical & Translational Med

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Section: Figurementioning

confidence: 99%

Altered microRNA expression in severe COVID‐19: Potential prognostic and pathophysiological role

Garnier

Pollet²,

Fourcot³

et al. 2022

Clinical & Translational Med

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“…Accumulating evidence indicates that miR-532-3p serves as a tumor suppressor or promoter in multiple human cancers, such as gastric, renal, and liver cancers [ 45 – 47 ]. It was also reported that miR-532-3p regulated proinflammation of macrophages [ 48 ]. In addition, inflammation-dependent downregulation of miR-532-3p can contribute to the progress of the sarcopenia [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analyses of Stromal-Immune Score-Related Competing Endogenous RNA Networks In Colon Adenocarcinoma

Tong

Peng

et al. 2022

Disease Markers

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Although recent clinical investigations emphasize the roles of myriad diversities of RNAs in stromal and immune components in the tumor microenvironment, especially in colon adenocarcinoma, however, analyses of “competing endogenous RNAs (ceRNA)” network in association with stromal and immune scores have yet to be determined. This study was conducted to explore the regulatory mechanisms of a stromal-immune score-based ceRNA network in colon adenocarcinoma. Stromal and immune scores of colon adenocarcinoma tumor samples were calculated by using the ESTIMATE algorithm. Differential expression analysis between samples with high/low stromal and immune scores was performed, followed by functional annotation for the overlapping DEmRNAs. The ceRNA network was constructed by differential expression analysis, prediction of RNA-RNA interaction, and correlation with clinicopathological parameters of the patients, which were further verified by external datasets and experiments. Colon adenocarcinoma patients having higher immune scores exhibited prolonged overall survival. RNA dataset analyses from TCGA revealed aberrant expressions of a total of 2052 mRNAs, 108 lncRNAs, and 70 miRNAs between high and low stromal/immune groups. Functional annotation mapped the differentially overexpressed mRNAs for immune-associated GO terms. To construct the ceRNA network, a total of 48 lncRNAs, 40 miRNAs, and 199 mRNAs were sorted out. A dysregulated ceRNA network consisting of 6 lncRNAs, 11 miRNAs, and 39 mRNAs was constructed by comparing RNA expressions between cancer as well as adjacent normal tissues. The ceRNA regulatory axis “MIAT/miR-532-3p/STC1” was regarded as a potential hit by the comprehensive analysis. The RT-qPCR assay showed upregulation of MIAT and STC1 while downregulation of hsa-miR-532-3p expression in cancer. Thus, our study highlights the potential role of a stromal-immune score-based ceRNA network in the colon adenocarcinoma microenvironment. The ceRNA axis MIAT/miR-532-3p/STC1 could serve as a promising therapeutic target for colon adenocarcinoma.

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“…hsa-miR-532-3p diminished the levels of ASK1 and downstream phosphorylation/translocation of p38 MAPK during LPS/TNFα induced inflammation in macrophages and reduced the release of various pro-inflammatory cytokines and chemokines, including IL-6 and TNF-alpha [75]. IL-6 is a proinflammatory cytokine that has been reported to be involved in the cytokine storm observed in severe COVID-19 [13].…”

Section: Discussionmentioning

confidence: 99%

Altered microRNA expression in severe COVID-19: potential prognostic and pathophysiological role

Garnier

Pollet

Fourcot

et al. 2022

Preprint

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Background The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is ongoing. The pathophysiology of SARS-CoV-2 infection is beginning to be elucidated but the role of microRNAs (miRNAs), small non-coding RNAs that regulate gene expression, remains incompletely understood. They play a role in the pathophysiology of viral infections with potential use as biomarkers. The objective of this study was to identify miRNAs as biomarkers of severe COVID-19 and to analyze their role in the pathophysiology of SARS-CoV-2 infection. Methods miRNA expression was measured in nasopharyngeal swabs from 20 patients with severe COVID-19, 21 patients with non-severe COVID-19 and 20 controls. Promising miRNAs to differentiate non-severe from severe COVID-19 patients were identified by differential expression analysis and sparse Partial Least Squares-Discriminant Analysis (sPLS-DA). ROC analysis, target prediction, GO enrichment and pathway analysis were used to analyze the role and the pertinence of these miRNAs in severe COVID-19. Results The number of expressed miRNAs was lower in severe COVID-19 patients compared to non-severe COVID-19 patients and controls. Among the differentially expressed miRNAs between severe COVID-19 and controls, 5 miRNAs were also differentially expressed between severe and non-severe COVID-19. sPLS-DA analysis highlighted 8 miRNAs, that allowed to discriminate the severe and non-severe COVID-19 cases. Target and functional analysis revealed enrichment for genes involved in viral infections and the cellular response to infection as well as one miRNA, hsa-miR-15b-5p, that targeted the SARS-CoV-2 RNA. The comparison of results of differential expression analysis and discriminant analysis revealed three miRNAs, namely hsa-miR-125a-5p, hsa-miR-491-5p and hsa-miR-200b-3p. These discriminated severe from non-severe cases with areas under the curve ranging from 0.76 to 0.80. Conclusions Our analysis of miRNA expression in nasopharyngeal swabs revealed several miRNAs of interest to discriminate severe and non-severe COVID-19. These miRNAs represent promising biomarkers and possibly targets for antiviral or anti-inflammatory treatment strategies.

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MicroRNA-532-3p Regulates Pro-Inflammatory Human THP-1 Macrophages by Targeting ASK1/p38 MAPK Pathway

Cited by 9 publications

References 43 publications

Altered microRNA expression in severe COVID‐19: Potential prognostic and pathophysiological role

Altered microRNA expression in severe COVID‐19: Potential prognostic and pathophysiological role

Comprehensive Analyses of Stromal-Immune Score-Related Competing Endogenous RNA Networks In Colon Adenocarcinoma

Altered microRNA expression in severe COVID-19: potential prognostic and pathophysiological role

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