MicroRNA‑520a suppresses the proliferation and mitosis of HaCaT cells by inactivating protein kinase B

Wang, Rui; Zhao, Zhiyun; Zheng, Liqiang; Xing, Xiaojing; Ba, Wei; Jun-fen, Zhang; Huang, Min; Zhu, Wenwei; Liu, Bing; Meng, Xianfu; Bai, Jia; Li, Cheng‐Xin; Li, Hengjin

doi:10.3892/etm.2017.5323

Cited by 7 publications

(8 citation statements)

References 35 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, it is known that the level of cyclin D1, a target gene of the Wnt/β-catenin pathway, increases with the progression from G0/G1 to S phase during the cell cycle (Prall et al, 1997). The phosphoinositide 3-kinase (PI3K)/Akt pathway plays a key role in the proliferation of several types of cells, including cancer cells, keratinocytes, and DPCs (Han et al, 2004;Hong et al, 2012;Wang et al, 2017). Previous studies have shown that minoxidil can prevent apoptosis of DPCs by activating Akt and delay the transition of the hair cycle into the regression phase by activating the Wnt/β-catenin pathway in DPCs (Han et al, 2004;Kwack et al, 2011).…”

mentioning

confidence: 99%

Vanillic Acid Stimulates Anagen Signaling via the PI3K/Akt/ β-Catenin Pathway in Dermal Papilla Cells

Kang

Choi

Koh

et al. 2020

Biomolecules & Therapeutics

View full text Add to dashboard Cite

Hair loss is a distressing disorder associated with various factors, including hormone imbalance, autoimmune diseases, stress, and chemotherapy. Several known types of hair loss are androgenetic alopecia (AGA), telogen effluvium, alopecia areata, and chemotherapy-induced alopecia (Cotsarelis and Millar, 2001; Hunt and McHale, 2005; Ito, 2010). Minoxidil (Rogain ®) and finasteride (Propecia ®) have been approved by the Food and Drug Administration (FDA) for treatment of alopecia. Minoxidil was developed as a vasodilator for the treatment of hypertension (Price, 1999). Although the mechanism of action of minoxidil is unclear, this drug stimulates hair growth by activating the Wnt/β-catenin pathway, opening the ATP-sensitive K + channels (KATP channels), upregulating the vascular endothelial growth factor (VEGF), and inhibiting apoptosis in dermal papilla cells (DPCs)

show abstract

mentioning

confidence: 99%

Vanillic Acid Stimulates Anagen Signaling via the PI3K/Akt/ β-Catenin Pathway in Dermal Papilla Cells

Kang

Choi

Koh

et al. 2020

Biomolecules & Therapeutics

View full text Add to dashboard Cite

show abstract

“…miR-520 is found in psoriatic keratinocytes and is markedly downregulated ( 10 ). In vitro experiments on HaCaT cells have confirmed the importance of miR-520 in the proliferation and mitosis of human keratinocytes ( 39 ). In vitro , it markedly suppressed the proliferation and mitotic entry of HaCaT cells by inhibiting AKT ( 40 ).…”

Section: Micrornas Involved In Psoriasismentioning

confidence: 91%

“…In vitro , it markedly suppressed the proliferation and mitotic entry of HaCaT cells by inhibiting AKT ( 40 ). miR-520a downregulates the transcription factor E2F, which suppresses cell cycle progression and proliferation ( 39 ). It also binds to the 3′UTR of AKT1 mRNA, thus inhibiting keratinocyte proliferation ( 40 ).…”

Section: Micrornas Involved In Psoriasismentioning

confidence: 99%

Understanding psoriasis: Role of miRNAs (Review)

Orăsan

2018

biom rep

View full text Add to dashboard Cite

Psoriasis is a chronic, immune-mediated inflammatory skin disease, with a multifactorial etiology and important immunologic, genetic and environmental components. Psoriasis vulgaris represents its most common form, with a variable prevalence across the globe. Although its pathogenesis remains to be fully elucidated, a lack of balance in the epigenetic network has been shown to trigger certain elements of this disease, possibly altering its outcome. MicroRNAs are small non-coding RNA molecules involved in RNA-silencing and the post-transcriptional regulation of gene expression, which also appear to mediate the immune dysfunction in psoriasis. Although microRNA research is a new field in dermatology and psoriasis, there is rapidly accumulating evidence for its major contribution in the pathogenesis of chronic inflammatory conditions, including psoriasis and other dermatological disorders. Furthermore, circulating miRNAs identified in patients' blood samples have been identified as promising biomarkers of diagnosis, prognosis or treatment response. Extended investigations in this field are required, as until now, the exact involvement of miRNAs in psoriasis have remained to be entirely elucidated. This short review highlights a number of the roles of miRNAs found in different stages of psoriasis.

show abstract

“…Substantial data associated with miRNAs, either upregulated or downregulated, and keratinocyte proliferation have been reported in psoriasis. The downregulated miRNAs include miR-125b, miR-181b-5p, miR-520a, miR-194, miR-217, miR-138, miR-320b, miR-150, miR-145-5p, miR-20a-3p, miR-876-5p, miR-99a, miR-187, miR-548a-3p, miR-330, and miR-146a AKT3 [47] miR-181b-5p AKT3, TRL4 [47,48] miR-520a AKT [49] miR-320b AKT3 [50] miR-194 GRHL2 [51] miR-217 [52] miR-138 hTERT [53] miR-150 HIF-1α, VEGFA [54] miR-145-5p MLK3 [55] miR-20a-3p SFMBT1 [56] miR-876-5p ANG-1 [57] miR-99a FZD5/FZD8 [58] miR-187 CD276 [59] miR-548a-3p PPP3R1 [60] miR-330 CTNNB1 [61] miR-146a EGFR Psoriasis, cSCC [62,63] miR-96-5p BNP3 Wound healing [64] miR-181a KRAS cSCC [29] miR-99b IGF-1R Condyloma acuminatum [65] miR-203 ∆Np63 Epidermodysplasia verruciformis [66] miR-130a STK40 [79] miR-122-5p SPRY2 [80] miR-223 PTEN [81] miR-17-3p MYOT Wound healing [82] miR-126 PLK2 [83] ↑: upregulation, ↓: downregulation.…”

Section: Keratinocyte Proliferationmentioning

confidence: 99%

“…Downregulation of miR-181b-5p also occurs in keratinocyte proliferation by targeting AKT3 [47]. AKT, known as protein kinase B, is also identified as a direct target of miR-520a [49]. The decreased expression of miR-320b promotes keratinocyte proliferation by enhancing AKT3 target [50].…”

Section: Psoriasismentioning

confidence: 99%

The Role of MicroRNAs in Epidermal Barrier

Lee

2020

IJMS

View full text Add to dashboard Cite

MicroRNAs (miRNAs), which mostly cause target gene silencing via transcriptional repression and degradation of target mRNAs, regulate a plethora of cellular activities, such as cell growth, differentiation, development, and apoptosis. In the case of skin keratinocytes, the role of miRNA in epidermal barrier integrity has been identified. Based on the impact of key genetic and environmental factors on the integrity and maintenance of skin barrier, the association of miRNAs within epidermal cell differentiation and proliferation, cell–cell adhesion, and skin lipids is reviewed. The critical role of miRNAs in the epidermal barrier extends the use of miRNAs for control of relevant skin diseases such as atopic dermatitis, ichthyoses, and psoriasis via miRNA-based technologies. Most of the relevant miRNAs have been associated with keratinocyte differentiation and proliferation. Few studies have investigated the association of miRNAs with structural proteins of corneocytes and cornified envelopes, cell–cell adhesion, and skin lipids. Further studies investigating the association between regulatory and structural components of epidermal barrier and miRNAs are needed to elucidate the role of miRNAs in epidermal barrier integrity and their clinical implications.

show abstract

MicroRNA‑520a suppresses the proliferation and mitosis of HaCaT cells by inactivating protein kinase B

Cited by 7 publications

References 35 publications

Vanillic Acid Stimulates Anagen Signaling via the PI3K/Akt/ β-Catenin Pathway in Dermal Papilla Cells

Vanillic Acid Stimulates Anagen Signaling via the PI3K/Akt/ β-Catenin Pathway in Dermal Papilla Cells

Understanding psoriasis: Role of miRNAs (Review)

The Role of MicroRNAs in Epidermal Barrier

Contact Info

Product

Resources

About