MicroRNA-497-5p inhibits proliferation and invasion of non-small cell lung cancer by regulating FGF2

Huang, Xiaori; Wang, Lei; Liu, Wei; Li, Fēi

doi:10.3892/ol.2019.9954

Cited by 35 publications

(31 citation statements)

References 32 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, miR‐23a‐3p overexpression inhibited mucosal melanoma cell malignancy and tumorigenicity . Furthermore, miR‐497‐5p was found reduced expression and its overexpression was found to suppress NSCLC cell growth via targeting FGF2 . EPHB6, belong to the Eph receptors family, that was found elevated expression in colorectal cancer and its overexpression accelerates colorectal cancer metastasis .…”

Section: Discussionmentioning

confidence: 99%

“…18 Furthermore, miR-497-5p was found reduced expression and its overexpression was found to suppress NSCLC cell growth via targeting FGF2. 19 EPHB6, belong to the Eph receptors family, that was found elevated expression in colorectal cancer and its overexpression accelerates colorectal cancer metastasis. 20 Furthermore, reduced EPHB6 expression was found in gastric carcinoma and associated with malignancy indicators for cancer.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

Kang

Shi

et al. 2019

BioFactors

View full text Add to dashboard Cite

Non‐small cell lung cancer (NSCLC) accounts for about 80% of lung cancers worldwide. In recent years, importance of noncoding RNAs including long noncoding RNA and microRNA in regulating tumor progression has been appreciated. Abnormally expression of DiGeorge syndrome critical region gene 5 (DGCR5) was found in multiple human cancers but its function in NSCLC is largely unknown. Reverse transcription quantitative polymerase chain reaction (RT‐qPCR) was conducted to explore DGCR5 expression level in NSCLC. Bioinformatic analyses were conducted to explore the targets of DGCR5. Cell counting kit‐8 assay, wound‐healing assay, and transwell invasion assay were performed to analyze functions of DGCR5. RT‐qPCR revealed that DGCR5 expression in NSCLC cells was significantly lower than in normal cell. DGCR5 overexpression suppresses NSCLC cell growth, migration, and invasion. Online algorithms found EPH receptor B6 (EPHB6) and DGCR5 contains same miR‐211‐5p binding region. The predicted connections were further validated by luciferase activity reporter assay. Recue experiments showed DGCR5 regulates NSCLC cell behaviors via targeting miR‐211‐5p/EPHB6. These findings collectively identified DGCR5/miR‐211‐5p/EPHB6 triple axis in NSCLC, which may novel understanding regarding the tumorigenesis of NSCLC.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

Kang

Shi

et al. 2019

BioFactors

View full text Add to dashboard Cite

show abstract

“…A previous study showed that the incidence of MODS in patients with paraquat poisoning was ~84% (6). miRNAs are regulatory factors that play crucial roles in regulating growth, maintaining homeostasis of organisms and mediating various pathophysiological processes of diseases, and have become important research topics (35)(36)(37). The present bioinformatics results suggested that miR-27a may be a potential regulator for IL-10, thus indicating the potential role of miR-27a in paraquat poisoning.…”

Section: Discussionmentioning

confidence: 61%

Clinical significance of serum levels of microRNA‑27a and its correlation with interleukin‑10 in patients with multiple organ dysfunction syndrome caused by acute paraquat poisoning

Dai

Zhang

et al. 2020

Exp Ther Med

View full text Add to dashboard Cite

The aims of the present study were to examine the clinical significance of serum microRNA-27a (miR-27a) levels in patients with multiple organ dysfunction syndrome (MODS) caused by acute paraquat poisoning and to investigate the correlation between miR-27a and interleukin (IL)-10. A total of 82 patients with MODS induced by acute paraquat poisoning and 88 healthy controls were recruited in the present study. Reverse transcription-quantitative PCR was used to measure serum miR-27a expression levels in patients with MODS and the control group. IL-10 serum levels were determined using ELISA. Decreased serum miR-27a level and increased IL-10 expression levels were detected in patients with paraquat poisoning compared with healthy controls (P<0.001). A moderately negative correlation was identified between the serum expression levels of miR-27a and IL-10 (r=-0.5225; P<0.001). miR-27a expression level was found to be associated with blood urea nitrogen, partial pressure of carbon dioxide, arterial blood lactic acid, and the acute physiology and chronic health evaluation II score (APACHE II; P<0.05). The area under the curve for miR-27a was 0.946, with a sensitivity of 86.6% and specificity of 87.5% at a cutoff value of 2.10. The non-survival patient group had lower miR-27a expression levels compared with the survival group (P<0.01). Multivariate Cox regression analyses suggested miR-27a expression level and APACHE II score were independent prognostic factors for 30-day mortality (P<0.01). The present results suggested that serum miR-27a level may be a potential novel diagnostic and prognostic factor for MODS caused by paraquat poisoning. Collectively, miR-27a may be involved in the process of MODS induced by paraquat poisoning by regulating the inflammatory response.

show abstract

“…CK‐8 (Dojindo, Kumamoto, Japan) solution was purchased to evaluate cell proliferation according to product instructions. The experimental procedure was performed based on a previous study …”

Section: Methodsmentioning

confidence: 99%

MicroRNA‐374b mediates the initiation of non‐small cell lung cancer by regulating ITGB1 and p53 expressions

et al. 2020

View full text Add to dashboard Cite

Background: Previous studies have shown that microRNAs (miRNAs) play important roles in the pathogenesis of human cancers. This study aims to clarify the role of miR-374b in non-small cell lung cancer (NSCLC). Methods: In this study, RT-qPCR and western blot analysis were used to measure mRNA and protein expression. The regulatory mechanism of miR-374b/ ITGB1 was investigated by dual-luciferase reporter, CCK-8, and transwell assays. Results: MiR-374b expression was reduced in NSCLC tissues and associated with lymph node metastasis, tumor stage and prognosis in NSCLC patients. Functionally, overexpression of miR-374b inhibited cell viability and metastasis in NSCLC. In addition, miR-374b blocked EMT and promoted p53 expression in NSCLC. MiR-374b was found to directly target ITGB1. Furthermore, upregulation of ITGB1 weakened the antitumor effect of miR-374b in NSCLC. Conclusions: MiR-374b inhibits the tumorigenesis of NSCLC by downregulating ITGB1 and upregulating p53.

show abstract

MicroRNA-497-5p inhibits proliferation and invasion of non-small cell lung cancer by regulating FGF2

Cited by 35 publications

References 32 publications

LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

LncRNA DGCR5 regulates the non‐small cell lung cancer cell growth, migration, and invasion through regulating miR‐211‐5p/EPHB6 axis

Clinical significance of serum levels of microRNA‑27a and its correlation with interleukin‑10 in patients with multiple organ dysfunction syndrome caused by acute paraquat poisoning

MicroRNA‐374b mediates the initiation of non‐small cell lung cancer by regulating ITGB1 and p53 expressions

Contact Info

Product

Resources

About