MicroRNA‑494‑3p protects rat cardiomyocytes against septic shock via PTEN

Wu, Peng; Kong, Lingchen; Li, Jianzhong

doi:10.3892/etm.2018.7116

Cited by 20 publications

(23 citation statements)

References 43 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have found that cell apoptosis plays an important role in sepsis-induced myocardial depression [ 6 ], but the mechanism which specifically regulates apoptosis in this context is still unclear. Notably, microRNAs (miRNAs) can regulate cell apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression

Zhu

Zhang

Wen

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Sepsis-induced myocardial depression has high mortality and is very common in intensive care units. Previous studies have found that microRNAs play an important role in regulating sepsis-induced myocardial depression. miR-150-5p is involved in many biological processes; however, the mechanism underlying its role in sepsis-induced myocardial depression is still unclear. In this study, we generated rat models of septic shock induced by lipopolysaccharide. Whole genomic RNA sequencing was performed on 12 left ventricles collected after LPS treatment to identify miRNAs. Most of the target genes of the differently expressed microRNAs were involved in apoptosis, according to Gene Ontology. We also observed apoptosis in the heart tissue and in H9C2 cardiomyocytes stimulated with lipopolysaccharide, indicating that cell apoptosis may be an important mechanism in sepsis-induced myocardial depression. Furthermore, the expression of miR-150-5p was reduced, and overexpression of miR-150-5p with mimics resulted in a decrease in apoptosis, decreased expression of cleaved caspase3 and Bax, and increased expression of Bcl-2. Additionally, after H9C2 cells were transfected with miR-150-5p mimics or an inhibitor, the expression of Akt2 decreased or increased, respectively. These findings suggest that miR-150-5p can alleviate apoptosis and may be a novel therapeutic target for sepsis-induced myocardial depression.

show abstract

Section: Introductionmentioning

confidence: 99%

Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression

Zhu

Zhang

Wen

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Wu et al found that also miR-494-3p targeted PTEN [ 60 ]. miR-494-3p is down-regulated in septic patients’ blood and its decrease correlates with the cardiac dysfunction.…”

Section: Resultsmentioning

confidence: 99%

“…It is a phosphatase that regulates various cellular signaling pathways and has tumor suppression properties. Some of the aforementioned studies demonstrated that PTEN is targeted by miRNAs in sepsis-induced cardiac damage [ 53 , 59 , 60 ]. A further complication in understanding the role of miRNAs in septic myocardial injury is represented by lncRNAs, as evidenced by some research studies mentioned above [ 45 , 73 , 74 ].…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs and Sepsis-Induced Cardiac Dysfunction: A Systematic Review

Manetti

Maiese

Paolo

et al. 2020

IJMS

View full text Add to dashboard Cite

Sepsis is a severe condition characterized by systemic inflammation. One of the most involved organs in sepsis is the heart. On the other hand, heart failure and dysfunction are some of the most leading causes of death in septic patients. miRNAs are short single-strand non-coding ribonucleic acids involved in the regulation of gene expression on a post-transcriptional phase, which means they are a part of the epigenetic process. Recently, researchers have found that miRNA expression in tissues and blood differs depending on different conditions. Because of this property, their use as serum sepsis biomarkers has also been explored. A narrative review is carried out to gather and summarize what is known about miRNAs’ influence on cardiac dysfunction during sepsis. When reviewing the literature, we found at least 77 miRNAs involved in cardiac inflammation and dysfunction during sepsis. In the future, miRNAs may be used as early sepsis-induced cardiac dysfunction biomarkers or as new drug targets. This could help clinicians to early detect, prevent, and treat cardiac damage. The potential role of miRNAs as new diagnostic tools and therapeutic strategies worth deepening the complex network between non-coding RNA and biological pathways. Additional studies are needed to further investigate their role in sepsis-induced myocardium injury.

show abstract

“…Another important finding in this research reflected that the elevation of miR-494 and knockdown of RIPK1 could accelerate the proliferation of neurons in the hippocampus of rats with Ep. Similarly, miR-494 has been revealed to be reduced in the temporal cortex of patients with mTLE [9], Wu et al have pointed out that the up-regulation of miR-494-3p was able to promote the proliferation in rat cardiomyocytes [30]. As for the effects of silenced RIPK1 in cell proliferation, Zhao et al have demonstrated that the reduction of RIPK1 has the capacity to promote the proliferation of myocardial cells in myocardial ischemia/ reperfusion (I/R) injury [31].…”

Section: Discussionmentioning

confidence: 97%

Overexpressed microRNA-494 represses RIPK1 to attenuate hippocampal neuron injury in epilepsy rats by inactivating the NF-κB signaling pathway

Qian

et al. 2020

Cell Cycle

View full text Add to dashboard Cite

Objective: The effects of microRNAs (miRNAs) have been identified in epilepsy (Ep) in recent years, our research was focused on the functions of miR-494 in Ep and its inner mechanisms. Methods: The Ep modeled rats induced by lithium chloride-pilocarpine were treated with agomir-miR-494 or RIPK1-siRNA. The pathology of rat hippocampal tissues was observed. Expression of miR-494, receptor-interacting protein kinase 1 (RIPK1) and nuclear factor-kappaB (NF-κB) p65 was assessed by RT-qPCR and Western blot analysis. The hippocampal neurons of epileptic rats were successfully modeled, which were transfected with miR-494 mimics or RIPK1-siRNA to determine neurons' proliferation ability and cell apoptosis. The target relation between miR-494 and RIPK1 was measured by bioinformatics website and dual luciferase gene reporter assay. Results: The expression of miR-494 was reduced, while the expression of RIPK1 and NF-κB p65 was amplified in hippocampus of Ep rats. Elevated miR-494 repressed the expression of RIPK1 to ameliorate the hippocampal neuron injury, accelerate neuronal proliferation, and restrain neuronal apoptosis via inactivating the NF-κB signaling pathway, causing a deceleration of Ep development. Furthermore, amplified RIPK1 was able to reverse the amelioration of neuronal injury in Ep rats which was contributed by upregulated miR-494. Conclusion:We found in this study that elevated miR-494 repressed RIPK1, causing an inactivation of the NF-κB signaling pathway and acceleration of cell proliferation, and suppression of apoptosis of hippocampal neurons in Ep rats, thereby attenuating the neuron injury and Ep development. Our research may provide novel targets for the therapy of Ep.

show abstract

MicroRNA‑494‑3p protects rat cardiomyocytes against septic shock via PTEN

Cited by 20 publications

References 43 publications

Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression

Overexpression of miR-150-5p Alleviates Apoptosis in Sepsis-Induced Myocardial Depression

MicroRNAs and Sepsis-Induced Cardiac Dysfunction: A Systematic Review

Overexpressed microRNA-494 represses RIPK1 to attenuate hippocampal neuron injury in epilepsy rats by inactivating the NF-κB signaling pathway

Contact Info

Product

Resources

About