MicroRNA-448 suppresses osteosarcoma cell proliferation and invasion through targeting EPHA7

Wu, Xiangkun; Liu, Yan; Liu, Yongxi; Xian, Wenfeng; Wang, Liuyu; Ding, Xunmeng

doi:10.1371/journal.pone.0175553

Cited by 29 publications

(23 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The finding of miR-885-5p to associate with fetal growth and sperm count also seems to demonstrate an association with development, which fits the developmental model for GCTs [70,71]. Meanwhile, miR-448 has been described as inhibiting cell proliferation and invasion in several tumor models [72][73][74], but its role in this specific context is still unclear. However, despite being of limited use in this specific discrimination, these microRNAs were found to be significantly upregulated in serum samples of patients with teratoma histology when compared to normal males, allowing for a good discrimination among the two groups, which fully confirms the prediction of the in vivo model.…”

Section: Discussionsupporting

confidence: 66%

Identification and Validation Model for Informative Liquid Biopsy-Based microRNA Biomarkers: Insights from Germ Cell Tumor In Vitro, In Vivo and Patient-Derived Data

Lobo

Gillis

Berg

et al. 2019

Cells

View full text Add to dashboard Cite

Liquid biopsy-based biomarkers, such as microRNAs, represent valuable tools for patient management, but often do not make it to integration in the clinic. We aim to explore issues impeding this transition, in the setting of germ cell tumors, for which novel biomarkers are needed. We describe a model for identifying and validating clinically relevant microRNAs for germ cell tumor patients, using both in vitro, in vivo (mouse model) and patient-derived data. Initial wide screening of candidate microRNAs is performed, followed by targeted profiling of potentially relevant biomarkers. We demonstrate the relevance of appropriate (negative) controls, experimental conditions (proliferation), and issues related to sample origin (serum, plasma, cerebral spinal fluid) and pre-analytical variables (hemolysis, contaminants, temperature), all of which could interfere with liquid biopsy-based studies and their conclusions. Finally, we show the value of our identification model in a specific scenario, contradicting the presumed role of miR-375 as marker of teratoma histology in liquid biopsy setting. Our findings indicate other putative microRNAs (miR-885-5p, miR-448 and miR-197-3p) fulfilling this clinical need. The identification model is informative to identify the best candidate microRNAs to pursue in a clinical setting.

show abstract

Section: Discussionsupporting

confidence: 66%

Identification and Validation Model for Informative Liquid Biopsy-Based microRNA Biomarkers: Insights from Germ Cell Tumor In Vitro, In Vivo and Patient-Derived Data

Lobo

Gillis

Berg

et al. 2019

Cells

View full text Add to dashboard Cite

show abstract

“…Fang et al reported that miR‐188‐5p suppresses tumour cell proliferation and metastasis by directly targeting FGF5 in hepatocellular carcinoma, implying that up‐regulated circMATR3 might sponge miR‐188‐5p and abolish its inhibitory effects on cell proliferation and metastasis. Similarly, Wu et al showed that elevated expression of miR‐448 suppressed osteosarcoma cell proliferation and invasion through targeting EPHA7, indicating that circMATR3 may reverse the inhibitory roles by absorbing miR‐448. Interestingly, miR‐188‐5p and miR‐448 have 3 common targeted mRNAs; among them, USP28 is a famous oncogene that is required for MYC stability in several human tumour cells, while MYC is a central regulator of cell growth, proliferation and apoptosis in many human cancers .…”

Section: Discussionmentioning

confidence: 94%

Effect of up‐regulation of circMATR3 on the proliferation, metastasis, progression and survival of hypopharyngeal carcinoma

Wang

Wei

et al. 2020

J Cellular Molecular Medi

View full text Add to dashboard Cite

Increasing number of circular RNAs (circRNAs) have been reported to play important role in gene regulation, carcinogenesis and pathogenesis in various cancers.However, the biological functions and underlying molecular mechanisms of circRNAs in hypopharyngeal squamous cell carcinoma (HSCC) remain elusive. Thus, secondary circRNA-seq profiling was performed to identify the differentially expressed circR-NAs between HSCC tissues and adjacent normal tissues, and the expression level of circMATR3 (derived from human gene matrin3 (MATR3), has_circRNA_0008922) was confirmed by qRT-PCR. Proliferation of HSCC cells was detected by cell counting kit-8 (CCK8) assay, apoptosis and the cell cycle were analysed by flow cytometry, and the migration and invasion of HSCC cells was determined by transwell assay.Bioinformatics analysis was conducted to predict possible pathways and potential miRNA targets of circMATR3. We found that circMATR3 was up-regulated in HSCC tissues, and abundant circMATR3 expression was markedly correlated with late T classification, advanced clinical stage, greater lymph node metastasis, and poor prognosis. Furthermore, knock-down of circMATR3 significantly inhibited proliferation, migration and invasion of HSCC cells, whereas silencing of circMATR3 induced cell apoptosis. Our analysis predicted that circMATR3 may participate in cancer-related pathways by serving as miRNA sponges. In conclusion, our findings first identified the oncogenic roles of circMATR3 in promoting the progression of HSCC and demonstrated that circMATR3 may be a novel prognostic marker and therapeutic target for HSCC. K E Y W O R D S circMATR3, circular RNAs, hypopharyngeal squamous cell carcinoma, progression S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Wang Z, Wei P, Wei D, et al. Effect of up-regulation of circMATR3 on the proliferation, metastasis, progression and survival of hypopharyngeal carcinoma. J Cell

show abstract

“…For example, miR‐448 suppressed cell metastasis by modulating the JAK1/STAT3 pathway in pancreatic ductal adenocarcinoma . In addition, miR‐448 was reported to suppress the proliferation and invasion of osteosarcoma cells by inhibiting EPHA7 . Besides that, miR‐448 was found to inhibit EMT by modulating ZEB1/2 in breast cancer .…”

Section: Discussionmentioning

confidence: 99%

microRNA‐448 inhibits the progression of non‐small–cell lung cancer through regulating IRS2

Gao

Feng

Wang

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

Recently , has been reported to be a tumor-associated miRNA in many human cancers. In this study, we investigated the function of miR-448 in non-small-cell lung cancer (NSCLC) progression and confirmed the relationship between miR-448 and insulin receptor substrates 2 (IRS2). First, downregulation of miR-448 and upregulation of IRS2 were detected in NSCLC using the quantitative real-time polymerase chain reaction (qRT-PCR) assay. Furthermore, the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay showed that miR-448 inhibited cell viability in NSCLC. Transwell and Western blot assays indicated that the upregulation of miR-448 inhibited cell metastasis and epithelial-to-mesenchymal transition (EMT) in NSCLC. And it was found that overexpression of miR-448 reduced the adhesion of A549 cells to HUVEC cells using the adhesion assay. Furthermore, the dual luciferase assay indicated that miR-448 directly targeted IRS2 in NSCLC. In addition, it was found that IRS2 silencing had an inhibitory effect on the progression of NSCLC, and the upregulation of IRS2 partially impaired the inhibitory effect of miR-448 in NSCLC. Briefly, overexpression of miR-448 inhibited cell proliferation, metastasis, and EMT by suppressing IRS2 expression in NSCLC. K E Y W O R D Sepithelial-to-mesenchymal transition, IRS2, metastasis, miR-448, non-small-cell lung cancer, proliferation

show abstract

MicroRNA-448 suppresses osteosarcoma cell proliferation and invasion through targeting EPHA7

Cited by 29 publications

References 34 publications

Identification and Validation Model for Informative Liquid Biopsy-Based microRNA Biomarkers: Insights from Germ Cell Tumor In Vitro, In Vivo and Patient-Derived Data

Identification and Validation Model for Informative Liquid Biopsy-Based microRNA Biomarkers: Insights from Germ Cell Tumor In Vitro, In Vivo and Patient-Derived Data

Effect of up‐regulation of circMATR3 on the proliferation, metastasis, progression and survival of hypopharyngeal carcinoma

microRNA‐448 inhibits the progression of non‐small–cell lung cancer through regulating IRS2

Contact Info

Product

Resources

About