Effect of up‐regulation of circMATR3 on the proliferation, metastasis, progression and survival of hypopharyngeal carcinoma

Abstract

Increasing number of circular RNAs (circRNAs) have been reported to play important role in gene regulation, carcinogenesis and pathogenesis in various cancers.However, the biological functions and underlying molecular mechanisms of circRNAs in hypopharyngeal squamous cell carcinoma (HSCC) remain elusive. Thus, secondary circRNA-seq profiling was performed to identify the differentially expressed circR-NAs between HSCC tissues and adjacent normal tissues, and the expression level of circMATR3 (derived from human gene matrin3 (MATR3), has_circRNA_0008922) was confirmed by qRT-PCR. Proliferation of HSCC cells was detected by cell counting kit-8 (CCK8) assay, apoptosis and the cell cycle were analysed by flow cytometry, and the migration and invasion of HSCC cells was determined by transwell assay.Bioinformatics analysis was conducted to predict possible pathways and potential miRNA targets of circMATR3. We found that circMATR3 was up-regulated in HSCC tissues, and abundant circMATR3 expression was markedly correlated with late T classification, advanced clinical stage, greater lymph node metastasis, and poor prognosis. Furthermore, knock-down of circMATR3 significantly inhibited proliferation, migration and invasion of HSCC cells, whereas silencing of circMATR3 induced cell apoptosis. Our analysis predicted that circMATR3 may participate in cancer-related pathways by serving as miRNA sponges. In conclusion, our findings first identified the oncogenic roles of circMATR3 in promoting the progression of HSCC and demonstrated that circMATR3 may be a novel prognostic marker and therapeutic target for HSCC. K E Y W O R D S circMATR3, circular RNAs, hypopharyngeal squamous cell carcinoma, progression S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Wang Z, Wei P, Wei D, et al. Effect of up-regulation of circMATR3 on the proliferation, metastasis, progression and survival of hypopharyngeal carcinoma. J Cell

A Web-Based Prediction Model for Cancer-Specific Survival of Elderly Patients With Hypopharyngeal Squamous Cell Carcinomas: A Population-Based Study

“…More than 50% of patients with HPSCC were usually diagnosed at a more advanced stage ( 21 ). Various factors lead to this result, such as minor symptoms in the early stages, early nodal metastasis, and poor differentiation ( 22 , 23 ). The prognosis of HPSCC is one of the worst for head and neck tumors.…”

Mutual regulation between N6-methyladenosine (m6A) modification and circular RNAs in cancer: impacts on therapeutic resistance

“…HSCC is a common malignant tumor originating from the mucous epithelium of the upper aerodigestive tract, accounting for about 5–15% of head and neck squamous cell carcinoma, and is characterized by hidden location, atypical early symptoms, high early metastasis rate and easy recurrence [ 187 , 188 ]. Consequently, identifying the mechanisms that upregulate progression and therapy tolerance at the molecular level will aid to improve the diagnosis and treatment of HSCC.…”

Research Progress of circRNAs in Head and Neck Cancers

“…Multiple circRNAs have been demonstrated to be involved in HNC development through the regulation of the proliferation process ( Figure 1 ). CircATRNL1 ( 40 ), hsa_circ_0007059 ( 41 ), circRNA_002178 ( 42 ), hsa_circ_0046263 ( 43 ), hsa_circ_0005379 ( 44 ), circUHRF1 ( 45 ), circPVT1 ( 46 , 47 ), circHIPK3 ( 48 , 49 ), circRNA_0000140 ( 50 ), circRNA_100290 ( 51 ), circDOCK1 ( 52 ), hsa_circ_0055538 ( 53 ), circITCH ( 54 ), hsa_circRNA_101036 ( 55 ), circCCND1 ( 20 ), circMYLK ( 56 ), CDR1as ( 17 , 57 , 58 ), circPKD2 ( 59 ), circLARP4 ( 60 ), hsa_circ_0042666 ( 61 ), hsa_circRNA_100533 ( 62 ), circMDM2 ( 63 ), circ_0067934 ( 64 ), circRPMS1 ( 65 ), circ-ZNF609 ( 66 , 67 ), hsa_circ_0072387 ( 68 ), circRASSF2 ( 38 ), hsa_circ_0023028 ( 69 ), circCTDP1 ( 70 ), circ_0008450 ( 71 ), circMATR3 ( 72 ), hsa_circ_0057481 ( 73 ), circ_0001742 ( 74 ), circ_0000218 ( 75 ), circ_0001971 ( 76 ), circCORO1C ( 77 ), hsa_circ_0109291 ( 78 ), hsa_circ_0036722 ( 34 ), hsa_circ_0002203 ( 79 ) and hsa_circ_0066755 ( 80 ) are associated with tumor proliferation ( Table 1 ).…”

“…CircRNAs in HNC have important roles in HNC metastasis and invasion ( Figure 2 ). Hsa_circ_0007059 ( 41 ), circUHRF1 ( 45 ), circHIPK3 ( 48 ), circRNA_0000140 ( 50 ), circRNA_100290 ( 51 ), hsa_circ_0055538 ( 53 ), CDR1as ( 17 , 57 ), circPKD2 ( 59 ), circLARP4 ( 60 ), hsa_circ_0042666 ( 61 ), hsa_circRNA_100533 ( 62 ), circ_0067934 ( 64 ), circRPMS1 ( 65 ), circ-ZNF609 ( 66 ), hsa_circ_0072387 ( 68 ), circRASSF2 ( 38 ), hsa_circ_0023028 ( 69 ), circCTDP1 ( 70 ), circ_0008450 ( 71 ), circMATR3 ( 72 ), hsa_circ_0057481 ( 73 ), circ_0001742 ( 74 ), circCORO1C ( 77 ), hsa_circ_0004491 ( 37 ), circ_0001971 ( 33 ), circFLNA ( 99 ), hsa_circ_0001162 ( 100 ), circARHGAP12 ( 101 ), circCRIM1 ( 102 ), circEPSTI1 ( 103 ), hsa_circ_0109291 ( 78 ), circRNA_002178 ( 42 ), hsa_circ_0002203 ( 79 ) and hsa_circ_0066755 ( 80 ) are involved in the regulation of HNC invasion and metastasis ( Table 2 ).…”