MicroRNA-26a inhibits osteosarcoma cell proliferation by targeting IGF-1

Tan, Xinyu; Fan, Shicai; Wu, Wen Shu; Yin, Zheng

doi:10.1038/boneres.2015.33

Cited by 31 publications

(15 citation statements)

References 27 publications

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“…miR-26a inhibits tumor growth of osteosarcoma and the stem cell-like phenotype by targeting Jagged1 [ 3 ]. miR-26a could also inhibit the proliferative abilities of osteosarcoma by targeting IGF-1 [ 71 ]. miR-126 promotes apoptosis and inhibits proliferation in osteosarcoma cells without significantly effecting cell cycle arrest at G1 phase [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs with prognostic significance in osteosarcoma: a systemic review and meta-analysis

et al. 2017

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IntroductionThis study aimed to elucidate the prognostic value of microRNAs (miRNAs) in patients with osteosarcoma.Materials and MethodsStudies were recruited by searching PubMed, Embase, the Cochrane Library, China National Knowledge Infrastructure, and Wanfang data-bases (final search update conducted January 2017). Eligible studies were identified and the quality was assessed using multiple search strategies.ResultsA total of 55 articles that investigated the correlation between miRNA expression and either patient survival or disease recurrence in osteosarcoma was initially identified. Among these, 30 studies were included in the meta-analysis. The results of our meta-analysis revealed that elevated levels of miR-21, miR-214, miR-29, miR-9 and miR-148a were associated with poor prognosis in osteosarcoma. Additionally, downregulated miR-382, miR26a, miR-126, miR-195 and miR-124 expression indicated poor prognosis in osteosarcoma.ConclusionsmiRNAs may act as independent prognostic factors in patients with osteosarcoma and are useful in stratifying risk.

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Section: Discussionmentioning

confidence: 99%

MicroRNAs with prognostic significance in osteosarcoma: a systemic review and meta-analysis

et al. 2017

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“…Recently the role of miR-26a in osteosarcoma has been investigated. [10][11][12][13][14] They found that overexpression of this miRNA accelerates cell cycle progression and inhibits apoptosis of MG63 and U2OS cells, two osteosarcoma cell line. However, the role of miR-26a-5p in osteosarcoma is completely unknown.…”

Section: Introductionmentioning

confidence: 99%

<p>microRNA-26a-5p Promotes Proliferation and Migration of Osteosarcoma Cells by Targeting <em>HOXA5</em> in vitro and in vivo</p>

Yu¹,

Chen²,

Zhang³

et al. 2019

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These authors contributed equally to this workBackground: Osteosarcoma is the most common primary malignant tumor of bone. However, the underlying pathogenic mechanisms are still unclear. miR-26a was an endogenous non-coding small RNAs that have been showed to play a critical role in regulating varieties of biological and pathological processes. In this study, we will investigate the function of miR-26a-5p in osteosarcoma cells. Methods: In this study, we explored the role of miR-26a-5p in osteosarcoma cell lines using qPCR, detected the proliferation, cell cycle and cell migration by CCK-8, PI and transwell. Results: We found that compared with noncancerous cells, miR-26a-5p was highly expressed in osteosarcoma cell lines, especially in U2OS cells. Overexpression of miR-26a-5p promotes cell proliferation, cell cycle, and cell migration, but inhibits cell apoptosis. But down-regulation of miR-26a-5p in U2OS cells exhibits opposite effects. We also confirmed that miR-26a-5p directly targets HOXA5 in U2OS cells. Overexpression of HOXA5 reversed the effect of miR-26a-5p on cell proliferation, migration, and apoptosis. Besides, we showed in that knock-down of miR-26a-5p or overexpression of HOXA5 increased cell sensitivity to chemotherapeutic drug paclitaxel. Conclusion: These findings indicate that highly expressed miR-26a-5p in osteosarcoma cells, and promotes proliferation and migration, but inhibits apoptosis of osteosarcoma cells by targeting HOXA5 which suggest that miR-26a-5p could serve as a novel therapeutic target for osteosarcoma.

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“…At present, >1,000 miRNAs have been identified in the human genome, and these can modulate the expression of thousands of human protein-encoding genes (9,13). Accumulated evidence shows that numerous miRNAs are abnormally expressed in several types of human cancer (14)(15)(16). These miRNAs are necessary to maintain the malignant phenotype of cancer cells, and can function as either tumor suppressors or oncogenes depending on the tumor type and effects of their target genes (17).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-129-5p suppresses cell proliferation, migration and invasion via targeting ROCK1 in osteosarcoma

Han

2018

Mol Med Report

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Osteosarcoma (OS) is the most common primary malignancy of the bone in teenagers and accounts for 20‑35% of all malignant primary bone tumors. Increasing evidence shows that microRNAs (miRNAs) are abnormally expressed in several types of human cancer. miRNAs are necessary to maintain the malignant phenotype of cancer cells and can function as either tumor suppressors or oncogenes. The present study aimed to measure the expression of miRNA‑129‑5p (miR‑129‑5p) in OS, determine the effects of miR‑129‑5p on the malignant behaviors of OS cells, and elucidate the molecular mechanism underlying the oncogenesis and progression of OS. The expression levels of miR‑129‑5p in OS tissues and cell lines were measured using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis. SAOS‑2 and U2OS cells were then transfected with miR‑129‑5p mimics or miR‑negative control. The effects of miR‑129‑5p on the proliferation, migration and invasion of SAOS‑2 and U2OS cells in vitro were then evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Transwell migration assay and invasion assays, respectively. In addition, bioinformatics analysis, a luciferase reporter assay, and RT‑qPCR and western blot analyses were used to examine whether Rho‑associated protein kinase 1 (ROCK1) was a direct target of miR‑129‑5p. The mRNA expression of ROCK1 in OS tissues was detected using RT‑qPCR analysis, and the biological roles of ROCK1 in OS cells were also evaluated. The results showed that miR‑129‑5p was significantly downregulated in the OS tissues and cell lines. The re‑expression of miR‑129‑5p suppressed the cell proliferation, migration and invasion of OS cells. In addition, ROCK1 was confirmed as a direct target of miR‑129‑5p. The mRNA expression of ROCK1 was high in OS tissues and inversely correlated with the expression of miR‑129‑5p. The downregulation of ROCK1 inhibited the proliferation, migration and invasion of OS cells. These findings suggested that miR‑129‑5p inhibited cell proliferation, migration and invasion in the development of OS via the negative regulation of ROCK1. The miR‑129‑5p/ROCK1 axis may serve as an efficient target in cancer therapy.

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MicroRNA-26a inhibits osteosarcoma cell proliferation by targeting IGF-1

Cited by 31 publications

References 27 publications

MicroRNAs with prognostic significance in osteosarcoma: a systemic review and meta-analysis

MicroRNAs with prognostic significance in osteosarcoma: a systemic review and meta-analysis

<p>microRNA-26a-5p Promotes Proliferation and Migration of Osteosarcoma Cells by Targeting <em>HOXA5</em> in vitro and in vivo</p>

MicroRNA-129-5p suppresses cell proliferation, migration and invasion via targeting ROCK1 in osteosarcoma

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