MicroRNA-25 promotes cell migration and invasion in esophageal squamous cell carcinoma

Xu, Xiaohui; Chen, Zhaoli; Zhao, Xiaohong; Wang, Jiwen; Ding, Derong; Wang, Zhen; Tan, Fengwei; Tan, Xiaogang; Zhou, Fang; Sun, Jian; Sun, Nan; Gao, Yibo; Shao, Kwang‐Tsao; Li, Ning; Qiu, Bin; He, Jie

doi:10.1016/j.bbrc.2012.03.048

Cited by 109 publications

(84 citation statements)

References 34 publications

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“…This information combined with the evidence provided by our mRNA and protein analysis on integrin expression in bulk cell lines and selected subpopulation of highly metastatic cells (ALDH high ), suggest that miR-25 could be a central player in F-actin organization and cytoskeletal dynamics. However, the observed miR-25-induced loss of a migratory phenotype, confirmed in selected ALDH high subpopulation of stem/progenitor-like cells, could not be fully explained by the acquisition of more epithelial characteristics (or blockage of EMT-like processes), despite the fact that Ecadherin is a validated target gene of miR-25 (50).…”

Section: Discussionmentioning

confidence: 86%

miR-25 Modulates Invasiveness and Dissemination of Human Prostate Cancer Cells via Regulation of αv- and α6-Integrin Expression

et al. 2015

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Altered microRNA (miRNA; miR) expression is associated with tumor formation and progression of various solid cancers. A major challenge in miRNA expression profiling of bulk tumors is represented by the heterogeneity of the subpopulations of cells that constitute the organ, as well as the tumor tissue. Here, we analyzed the expression of miRNAs in a subpopulation of epithelial stem/progenitor-like cells in human prostate cancer [prostate cancer stem cell (PCSC)] and compared their expression profile to more differentiated cancer cells. In both cell lines and clinical prostate cancer specimens, we identified that miR-25 expression in PCSCs was low/absent and steadily increased during their differentiation into cells with a luminal epithelial phenotype. Functional studies revealed that overexpression of miR-25 in prostate cancer cell lines and selected subpopulation of highly metastatic and tumorigenic cells (ALDH high ) strongly affected the invasive cytoskeleton, causing reduced migration in vitro and metastasis via attenuation of extravasation in vivo. Here, we show, for the first time, that miR-25 can act as a tumor suppressor in highly metastatic PCSCs by direct functional interaction with the 3 0 -untranslated regions of proinvasive a vand a 6 -integrins. Taken together, our observations suggest that miR-25 is a key regulator of invasiveness in human prostate cancer through its direct interactions with a v -and a 6 -integrin expression. Cancer Res; 75(11); 2326-36. Ó2015 AACR.

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Section: Discussionmentioning

confidence: 86%

miR-25 Modulates Invasiveness and Dissemination of Human Prostate Cancer Cells via Regulation of αv- and α6-Integrin Expression

et al. 2015

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“…Hummel et al (56) revealed that miR-425 was also upregulated in esophageal carcinoma cells following short-or long-term treatment with cisplatin. miR-25, which is overexpressed in ovarian cancer, gastric cancer and esophageal squamous cell carcinoma, promotes tumorigenesis and metastasis by targeting Bim, reversion-inducing cysteine-rich protein with Kazal motifs and cadherin 1, respectively (57)(58)(59).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma

Li¹,

Zhang²,

Liu³

et al. 2015

Oncology Letters

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Abstract. MicroRNAs (miRNAs) are a family of small non-protein coding RNAs, which regulate the expression of a wide variety of genes at the post-transcriptional level to control numerous biological and pathological processes. Various circulating miRNAs have been identified as potential diagnostic and prognostic biomarkers in multiple types of cancer and disease. The aim of the present study was to identify potential miRNA biomarkers for the early diagnosis and relapse prediction of osteosarcoma (OS). miRNA profiling was performed on serum from patients with osteosarcoma and healthy controls. All putative miRNAs were verified by reverse transcription-quantitative polymerase chain reaction analysis of 20 pre-therapeutic OS patients and 20 healthy individuals. The expression of miR-106a-5p, miR16-5p, miR-20a-5p, miR-425-5p, miR451a, miR-25-3p and miR139-5p was demonstrated to be downregulated in the serum of OS patients when compared with that of the healthy controls. Receiver-operating characteristic curve analyses indicated that these 7 miRNAs may be used as diagnostic biomarkers with the ability to discriminate between the healthy cohort and patients with OS. These results provide novel insights into the use of miRNAs in early blood screening for OS.

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“…p53 acts as a tumor by triggering cell cycle arrest and apoptosis, as well as maintaining genome stability (26). Xu et al (27) revealed that miR-25 promoted cell migration and invasion in ESCC. In prostate cancer and ovarian cancer, miR-93 can bind to and decrease PTEN expression, thereby promoting cancer cell proliferation and invasion (28,29).…”

Section: Discussionmentioning

confidence: 99%

Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster

Wang

Yang

Jin³

et al. 2017

Exp Ther Med

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Abstract. MicroRNAs (miRNAs/miRs) are a class of small, non-coding RNA molecules that serve a key function in carcinogenesis and tumor progression. Recent evidence indicates that miRNAs may act as powerful regulators of migration and invasion. The present study aimed to investigate the effect of miR-25 on the invasion and metastasis of KYSE-150 and EC109 esophageal squamous cell carcinoma (ESCC) cells, and predict the mechanism of this effect by bioinformatically analyzing the miR-106b-25 cluster. In order to alter the expression of miR-25 in the two cell lines, a miR-25 inhibitor or mimic were transfected into the cells, which were then studied via Transwell migration and invasion assays. Subsequently, the target genes of the miR-106b-25 cluster were predicted using miRanda, PicTar, TargetScan and miRTarbase, and the functions of the target genes were predicted via Gene Ontology term and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Then, a protein-protein interaction (PPI) network was produced using the Search Tool for the Retrieval of Interacting Genes. The results revealed that overexpressing miR-25 led to significantly increased cell migration and invasion in KYSE150 and EC109 cells. Suppressing miR-25 resulted in significantly decreased cell migration and invasion in KYSE150 cells, while the result was not significant in EC109 cells. Target genes of the miR-106b-25 cluster were significantly enriched in the biological process regulation of cellular metabolic process and several cancer-associated pathways, such as those for glioma and melanoma. The PPI network revealed that PTEN, TP53, MDM2, E2F1, PRMT5, MCM2, RB1, CDKN1A, SHAD7 and EZH2 may serve core roles within the network and associate with one another during the pathogenesis of ESCC. These results indicate that a high expression of miR-25 promotes the invasion and metastasis of ESCC cells, while the influence of low expression of miR-25 differs with cells with different degrees of differentiation. Invasion and metastasis are not effected in cells with poor differentiation, while they were decreased in well differentiated cells. Furthermore, PTEN, TP53, MDM2, E2F1, PRMT5, MCM2, RB1, CDKN1A, SHAD7 and EZH2 may be targeted by the miR-106b-25 cluster, and act together to regulate the development of ESCC. IntroductionEsophageal cancer is an important public health problem worldwide, as the eighth most common cancer and sixth most common cause of cancer-associated mortality (1). Esophageal squamous cell carcinoma (ESCC) accounts for 90% of all histological types of esophageal carcinoma in the highest risk area, North-central China, which ranks first in ESCC morbidity and mortality rates (2). Genetic heterogeneity and complex regulatory networks make it difficult to treat ESCC (3). Thus, identifying the regulatory pathways for the stratification of ESCC is of great clinical significance.MicroRNAs (miRNAs/miRs) are single-stranded RNAs of 18-24 nucleotides in length that post-transcriptionally regulate gene expression by directly bindin...

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MicroRNA-25 promotes cell migration and invasion in esophageal squamous cell carcinoma

Cited by 109 publications

References 34 publications

miR-25 Modulates Invasiveness and Dissemination of Human Prostate Cancer Cells via Regulation of αv- and α6-Integrin Expression

miR-25 Modulates Invasiveness and Dissemination of Human Prostate Cancer Cells via Regulation of αv- and α6-Integrin Expression

MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma

Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster

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