MicroRNA-223 Is Upregulated in Active Tuberculosis Patients and Inhibits Apoptosis of Macrophages by Targeting FOXO3

Xi, Xiue; Zhang, Chunxiao; Han, Wei; Zhao, Huayang; Zhang, Huiqiang; Jiao, Junhua

doi:10.1089/gtmb.2015.0090

Cited by 51 publications

(39 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In support of this, the overexpression of miR-223 in isolated human macrophages reduced apoptosis and suppressed FOXO3 protein levels. The miR-223 inhibitory effect on apoptosis was counteracted by FOXO3 overexpression 71 . Finally, expression levels of miR-155 are increased in renal tissues of rats that have undergone ischemia/reperfusion injury as well as in hypoxia/reoxygenation injury-induced human kidney proximal tubules epithelial (HK2) cells 70 .…”

Section: Microrna-mediated Regulation Of Foxo3mentioning

confidence: 99%

“…The opposite effects were observed when miR-34a levels were reduced. Other miRNA targets mediating apoptosis via FOXO3 include miR-223 and miR-155 69– 71 . Apoptosis of peripheral blood macrophages is decreased in patients with tuberculosis, while isolated human macrophages transfected with mycobacterium tuberculosis (Mtb) strain H37Rv displayed an increase in endogenous miR-223 levels.…”

Section: Microrna-mediated Regulation Of Foxo3mentioning

confidence: 99%

See 1 more Smart Citation

Recent advances in understanding the role of FOXO3

et al. 2018

View full text Add to dashboard Cite

The forkhead box O3 (FOXO3, or FKHRL1) protein is a member of the FOXO subclass of transcription factors. FOXO proteins were originally identified as regulators of insulin-related genes; however, they are now established regulators of genes involved in vital biological processes, including substrate metabolism, protein turnover, cell survival, and cell death. FOXO3 is one of the rare genes that have been consistently linked to longevity in in vivo models. This review provides an update of the most recent research pertaining to the role of FOXO3 in (i) the regulation of protein turnover in skeletal muscle, the largest protein pool of the body, and (ii) the genetic basis of longevity. Finally, it examines (iii) the role of microRNAs in the regulation of FOXO3 and its impact on the regulation of the cell cycle.

show abstract

Section: Microrna-mediated Regulation Of Foxo3mentioning

confidence: 99%

Section: Microrna-mediated Regulation Of Foxo3mentioning

confidence: 99%

Recent advances in understanding the role of FOXO3

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Made up of short nucleotide sequences, miRNAs can be readily targeted using engineered oligonucleotides, as evidenced by the effect of miR-122 inhibitor miravirsen which has shown efficacy in chronic Hepatitis C infection and is currently being evaluated in a phase 2 clinical trial [14,183]. Several miRNAs are upregulated in Mtb infection [184][185][186], including those involved in regulation of important anti-mycobacterial processes such as autophagy [187,188], apoptosis [189,190] and cytokine production [191]. Given the relative ease of designing inhibitors to miRNAs, it is likely that they will provide an attractive target for manipulating the host response to Mtb infection in future HDT development [182].…”

Section: Micrornas (Mirs)mentioning

confidence: 99%

Sharpening nature's tools for efficient tuberculosis control: A review of the potential role and development of host-directed therapies and strategies for targeted respiratory delivery

O'Connor

Gleeson

Fagan-Murphy

et al. 2016

Advanced Drug Delivery Reviews

View full text Add to dashboard Cite

“…5 A recent study revealed the differential miRNA expression in active and latent TB infection. [8][9][10] However, the detailed regulation mechanism of miRNAs in immune response during Mtb infection is not yet known. [8][9][10] However, the detailed regulation mechanism of miRNAs in immune response during Mtb infection is not yet known.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of miR‐20b‐5p facilitates Mycobacterium tuberculosis survival in RAW 264.7 macrophages via attenuating the cell apoptosis by Mcl‐1 upregulation

Zhang

2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb). The interaction between Mtb and macrophages, which is regulated by microRNAs, determines the development of TB. However, the function of microRNA‐20b‐5p (miR‐20b‐5p) in RAW 264.7 macrophages against Mtb remains unknown. In this study, we analyzed the expression level of miR‐20b‐5p in macrophage responses to Mtb infection and exosomes derived from macrophages after Mtb infection. MiR‐20b‐5p mimics and inhibitor were, respectively, transfected to evaluate the effect of miR‐20b‐5p on Mtb and macrophages. In addition, the targets of miR‐20b‐5p were predicted by a bioinformatics analysis. The macrophages were respectively transfected with miR‐20b‐5p mimics and inhibitor to determine the messenger RNA expression levels of the targets by reverse transcription‐polymerase chain reaction assay. The results revealed that the miR‐20b‐5p expression level was decreased in the infected macrophages at different times. MiR‐20b‐5p was shown in the exosomes released from macrophages infected with Mtb. Upregulation of the miR‐20b‐5p level suppressed the survival of Mtb in macrophages, while downregulation of the miR‐20b‐5p level enhanced the survival of Mtb in macrophages. Overexpression of miR‐20b‐5p decreased the cell viability and induced apoptosis in Mtb‐infected macrophages, while underexpression of miR‐20b‐5p increased the cell vitality and attenuated apoptosis in Mtb‐infected macrophages. The bioinformatics analysis revealed that Mcl‐1 was a target of miR‐20b‐5p. MiR‐20b‐5p negatively regulated the expression of Mcl‐1. Overall, this study is the first to demonstrate the effect of miR‐20b‐5p on Mtb infection and present miR‐20b‐5p and exosomes as the potential therapeutic targets of TB.

show abstract

MicroRNA-223 Is Upregulated in Active Tuberculosis Patients and Inhibits Apoptosis of Macrophages by Targeting FOXO3

Abstract: Our data provide new clues for the essential role of miR-223 in the regulation of anti-Mtb-directed immune responses, which relies on the regulation of FOXO3 expression.

Cited by 51 publications

References 34 publications

Recent advances in understanding the role of FOXO3

Recent advances in understanding the role of FOXO3

Sharpening nature's tools for efficient tuberculosis control: A review of the potential role and development of host-directed therapies and strategies for targeted respiratory delivery

Downregulation of miR‐20b‐5p facilitates Mycobacterium tuberculosis survival in RAW 264.7 macrophages via attenuating the cell apoptosis by Mcl‐1 upregulation

Contact Info

Product

Resources

About