microRNA-221 and microRNA-18a identification in stool as potential biomarkers for the non-invasive diagnosis of colorectal carcinoma

Yau, Tung On; Wu, Chung Wah; Dong, Yujuan; Tang, C.H.K.; Ng, Simon Siu Man; Chan, Francis K.L.; Sung, Joseph J.Y.; Yu, Jun

doi:10.1038/bjc.2014.484

Cited by 107 publications

(93 citation statements)

References 32 publications

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“…miR-18a belongs to the miR17-92 gene cluster, of which HIF-1α is a target gene (16). It has been reported that miR-18a serves a crucial function in the occurrence and development of myocardial ischemia (17), colonic neoplasm (18), liver tumors (19), prostate cancer (20) and malignant gliomas (21). Furthermore, a previous study suggests that miR-18a may be a specific biomarker for the early diagnosis and treatment of tumors (22).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-18a regulates invasive meningiomas via hypoxia-inducible factor-1α

Gao

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate the effects of microRNA-18a (miR-18a) on the invasiveness and metastasis of invasive meningiomas and the underlying mechanism. A total of 69 patients with meningiomas (30 patients in the invasive meningioma group and 39 patients in the non-invasive meningioma group) and 48 cases in the control group were enrolled. Samples of meningioma tissues, serum and cerebrospinal fluid were collected. Reverse transcription-quantitative polymerase chain reaction was performed to quantify the expression levels of hypoxia-inducible factor-1α (HIF-1α) mRNA and miR-18a. Western blot analysis was used to determine protein expression levels of HIF-1α. The expression levels of HIF-1α mRNA and protein in all three types of sample from the invasive meningioma group were significantly higher compared with those in the control and non-invasive meningioma groups (P<0.05), and the expression levels of HIF-1α mRNA in the serum and cerebrospinal fluid of the non-invasive meningioma group were significantly higher compared with those in the control group (P<0.05). The expression levels of miR-18a in the invasive meningioma group were significantly reduced compared with those in the control and non-invasive meningioma groups (P<0.05), whereas the levels of miR-18a in the non-invasive meningioma group were significantly lower compared with those in the control group (P<0.05). The expression of HIF-1α is significantly upregulated in patients with invasive meningiomas, possibly due to the downregulation of miR-18a expression. Therefore, miR-18a may regulate invasive meningiomas via HIF-1α.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-18a regulates invasive meningiomas via hypoxia-inducible factor-1α

Gao

et al. 2015

Experimental and Therapeutic Medicine

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“…miR-221 belongs to the miR-221/222 family, which locates on the X chromosome and shares some identical seed sequences with its homologous miRNA, miR-222 [14]. Accumulating evidence has indicated that miR-221 was an oncogene, which was up-regulated in a variety of cancers, including hepatocellular carcinoma, prostate adenocarcinoma, and colorectal carcinoma [15][16][17][18]. These results indicate that miR-221 may play significant roles in cancer development.…”

Section: Introductionmentioning

confidence: 99%

MiR-221 Promotes Capan-2 Pancreatic Ductal Adenocarcinoma Cells Proliferation by Targeting PTEN-Akt

Yang

Xia

et al. 2016

Cell Physiol Biochem

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Background/Aims: MicroRNAs (miRNAs, miRs) have emerged as critical regulators of cancer cell proliferation. The effect of miR-221 on cancer cell growth could be significantly changeable in different cell lines. Although miR-221 was reported to promote the cell growth of pancreatic ductal adenocarcinoma (PDAC) cells, its role in Capan-2 cell line is largely unknown. Methods: Capan-2 cells were transfected with miR-221 mimics, inhibitors, or negative controls. Cell Counting Kit-8 was used to determine cell viability. EdU staining and cell cycle analysis were used to measure cell proliferation. Western blotting was used to detect the expression levels of PTEN and phospho-Akt. The PI3K-Akt pathway activator SC-79 and inhibitor LY294002 were used to perform the rescue experiment in determining cell proliferation. Results: Overexpressing miR-221 significantly increased cell vitality and promoted cell proliferation and G1-to-S phase transition of the cell cycle in Capan-2 cells, while inhibition of miR-221 decreased that. The protein level of PTEN in Capan-2 cells was downregulated by overexpressing miR-221, while upregulated by inhibiting miR-221. Consistently, enhanced phosphorylation of Akt^Ser473 was observed in miR-221 overexpressed Capan-2 cells, and the opposite result was found in miR-221 inhibited cells. LY294002 restored the pro-proliferation effect of miR-221 on Capan-2 cells, while SC-79 had no additional effect on cell proliferation in Capan-2 cells transfected with miR-221 mimics. Conclusion: Our study indicates that miR-221 is an oncogenic miRNA which promotes Capan-2 cells proliferation by targeting PTEN-Akt pathway.

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“…These small RNAs impact many cellular processes and their deregulation is causative of many human cancers. In addition, many miRNAs were reported as predictive biomarkers and therapeutic targets in several cancers (17)(18)(19)(20).…”

Section: Discussionmentioning

confidence: 99%

Differential microRNA expression profiles in HCT116 colorectal cancer cell lines located in the lung and colon

Wang

Jiang

et al. 2014

Molecular Medicine Reports

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Colorectal cancer (CRC) is one of the most common types of cancer worldwide. The majority of mortalities caused by colorectal cancer are due to metastatic disease. As numerous CRC patients experience metastasis to the liver or lung and fail to respond to curative therapies, intensive research efforts have sought to identify the molecular changes or regulatory mechanisms underlying CRC metastasis. In the present study, a stable CRC cell line, HCT16, overexpressing firefly luciferase was constructed and an in vivo metastasis model was established via intravenous injection of this cell line. Using an imaging system, tumor tissue located in the lung and colon was separated and cells were prepared. The microRNA (miRNA) expression profiles of these lung homing or colon homing cells were assessed and compared. A total of 38 differentially expressed miRNAs were selected and confirmed our previous results; several of these have been reported to be involved in the regulation of cancer progression. However, the remaining miRNAs require further investigation. The present profiling may be the first step toward delineating the differential expression of miRNAs in the CRC cells located in the colon and the lung, enabling the elucidation of the regulation associated with miRNAs in colorectal lung metastases. These miRNAs require further validation and functional analysis to evaluate whether they are important in the pathogenesis of colorectal lung metastases or are adopted as markers to predict colorectal metastasis.

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microRNA-221 and microRNA-18a identification in stool as potential biomarkers for the non-invasive diagnosis of colorectal carcinoma

Cited by 107 publications

References 32 publications

MicroRNA-18a regulates invasive meningiomas via hypoxia-inducible factor-1α

MicroRNA-18a regulates invasive meningiomas via hypoxia-inducible factor-1α

MiR-221 Promotes Capan-2 Pancreatic Ductal Adenocarcinoma Cells Proliferation by Targeting PTEN-Akt

Differential microRNA expression profiles in HCT116 colorectal cancer cell lines located in the lung and colon

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