MicroRNA-18a regulates invasive meningiomas via hypoxia-inducible factor-1α

Abstract

Abstract. The aim of the present study was to investigate the effects of microRNA-18a (miR-18a) on the invasiveness and metastasis of invasive meningiomas and the underlying mechanism. A total of 69 patients with meningiomas (30 patients in the invasive meningioma group and 39 patients in the non-invasive meningioma group) and 48 cases in the control group were enrolled. Samples of meningioma tissues, serum and cerebrospinal fluid were collected. Reverse transcription-quantitative polymerase chain reaction was performed to quantify the expression levels of hypoxia-inducible factor-1α (HIF-1α) mRNA and miR-18a. Western blot analysis was used to determine protein expression levels of HIF-1α. The expression levels of HIF-1α mRNA and protein in all three types of sample from the invasive meningioma group were significantly higher compared with those in the control and non-invasive meningioma groups (P<0.05), and the expression levels of HIF-1α mRNA in the serum and cerebrospinal fluid of the non-invasive meningioma group were significantly higher compared with those in the control group (P<0.05). The expression levels of miR-18a in the invasive meningioma group were significantly reduced compared with those in the control and non-invasive meningioma groups (P<0.05), whereas the levels of miR-18a in the non-invasive meningioma group were significantly lower compared with those in the control group (P<0.05). The expression of HIF-1α is significantly upregulated in patients with invasive meningiomas, possibly due to the downregulation of miR-18a expression. Therefore, miR-18a may regulate invasive meningiomas via HIF-1α.

The dual functional role of MicroRNA‐18a (miR‐18a) in cancer development

“…Additionally, this research group indicated that miR‐18a potentially increases radiosensitivity by targeting ataxia telangiectasia mutated (ATM) and hypoxia‐inducible factor 1 alpha (HIF‐1α) in lung cancer cells and CD133 + stem cells. A similar mechanism found in BC [87,88], CC [89], CRC [90], GC [91] and meningiomas [92], supporting the hypothesis that miR‐18a enhances radiosensitivity by downregulating both ATM and HIF‐1α. Collectively, these findings indicate that miR‐18a could be a promising radiosensitizer and could improve the benefit of radiotherapy for patients with a broad range of human cancers.…”

MicroRNA-135a participates in the development of astrocytes derived from bacterial meningitis by downregulating HIF-1α

“…A previous study concluded that, in a fetus infected with bovine viral diarrhea, the virus exhibited an elevated expression of HIF-1␣ (4). HIF-1␣ has also been demonstrated to be upregulated by the inhibition of miR-18a in invasive meningioma (25). Moreover, a previous study indicated that HIF-1␣ is upregulated in meningioma, thus highlighting a correlation with the malignancy of menin- Fig.…”

MicroRNA-221/222 Inhibits the Radiation-Induced Invasiveness and Promotes the Radiosensitivity of Malignant Meningioma Cells

“…Genome-wide studies have demonstrated that miRNA genes are frequently located in cancer-associated genomic regions, indicating the potential roles of miRNAs in tumorigenesis ( 17 ). Previous studies on meningioma have suggested that several miRNAs participate in the regulation of cell proliferation ( 18 – 21 ), apoptosis ( 19 , 22 ), invasiveness ( 19 , 23 ), migration ( 19 , 24 ), tumor recurrence ( 25 – 27 ), and histopathological progression ( 18 , 19 , 25 , 27 – 29 ). However, no miRNAs have been verified to affect the radiosensitivity of meningiomas.…”