MicroRNA‐218‐5p relieves postmenopausal osteoporosis through promoting the osteoblast differentiation of bone marrow mesenchymal stem cells

Abstract

MicroRNAs (miRs) are short noncoding RNAs that play key regulatory roles in osteoblast differentiation. In this study, the specific regulatory roles of miR‐218‐5p on postmenopausal osteoporosis (PMOP) were investigated. The mouse model of PMOP was established by bilateral ovariectomy, and the injection of miR‐218‐5p mimics significantly relieved PMOP degree. Then, bone marrow mesenchymal stem cells (BMMSCs) isolated from PMOP mice were induced into osteoblasts. When compared with normal BMMSCs, PMOP BMMSCs exhibited significantly lower alkaline phosphatase (ALP) activity and less mineralized nodules, as well as downregulated miR‐218‐5p, Runx2, Osterix, COL1A1, and OCN after induction (P < .05). The transfection of miR‐218‐5p mimics, and inhibitor significantly promoted, inhibited the osteoblast differentiation of PMOP BMMSCs, respectively. In addition, COL1A1 was a target of miR‐218‐5p. The transfection of miR‐218‐5p mimics into PMOP BMMSCs significantly upregulated COL1A1 at 14th and 21st day post‐induction, but not at 7th day. Our findings suggest miR‐218‐5p may relieve PMOP through promoting the osteoblast differentiation of BMMSCs.

LncRNA LINC00511 promotes COL1A1-mediated proliferation and metastasis by sponging miR-126-5p/miR-218-5p in lung adenocarcinoma

“…Bioinformatic analysis showed that the COL1A1 3’-UTR contains binding sites for miR-126-5p and miR-218-5p (Fig. 5 C), consistent with previous studies [ 25 ]. To verify the endogenous mutual interactions between COL1A1 and both miR-126-5p and miR-218-5p, we constructed COL1A1 3’-UTR WT and COL1A1 3’-UTR MUT plasmids.…”

Extracellular vesicles from deciduous pulp stem cells recover bone loss by regulating telomerase activity in an osteoporosis mouse model

“…Multiple miRNAs regulate the osteogenic differentiation of BMMSCs [40]. For example, microRNA-218-5p targets COL1A1 to promote osteogenic differentiation of OVX-BMMSCs in vitro [41]; while microRNA-145 suppresses the osteogenic differentiation of human BMMSCs [42]. In this study, RNasepretreated SHED-EVs attenuated the SHED-EV-mediated rescue of Tert-associated telomerase activity in recipient BMMSCs.…”

Betulinic Acid Protects From Bone Loss in Ovariectomized Mice and Suppresses RANKL-Associated Osteoclastogenesis by Inhibiting the MAPK and NFATc1 Pathways

“…This involves reduced bone mass with excessive activation of osteoclasts (e.g., Paget's disease) commonly seen in osteoporosis and bone metastases (e.g., multiple myeloma) (Tsukasaki and Takayanagi, 2019). In cases with postmenopausal osteoporosis, bone mass loss is mainly caused by reduced estrogen accompanied by increased osteoclasts and elevated bone resorptive activity (Kou et al, 2019). In this study, we demonstrated that BA in mice with osteoporosis induced by OVX prevented bone loss.…”