MicroRNA-212 Regulates the Expression of Olfactomedin 1 and C-Terminal Binding Protein 1 in Human Endometrial Epithelial Cells to Enhance Spheroid Attachment In Vitro1

Kottawatta, Kottawattage S.A.; So, Kam-Hei; Kodithuwakku, Suranga P.; Ng, Ernest H.Y.; Yeung, William S.B.; Lee, Ckf

doi:10.1095/biolreprod.115.131334

Cited by 24 publications

(18 citation statements)

References 57 publications

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“…Hormones, growth factors, cytokines, and lipid messengers are involved in the dialog. For instance, hCG stimulates miR‐212, which in turn decreases the expression of olfactomedin in endometrial epithelial cells favoring embryo attachment …”

Section: Mirnas In Endometriummentioning

confidence: 99%

MicroRNA and Embryo Implantation

Liu

Niu

et al. 2015

American J Rep Immunol

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Problem In mammals, implantation involves interactions between an activated blastocyst and a receptive endometrium. There are controversies on the role of microRNAs in preimplantation embryo development. The actions of endometrial microRNAs on implantation are beginning to be understood. Method of Study Review of literature on microRNAs in preimplantation embryos and endometrium. Results Emerging evidence suggests a role of microRNAs in blastocyst activation and implantation. Differential expression of microRNAs is found between receptive and non‐receptive endometria. Members of the let‐7, miR‐200, miR‐30 families, and the miR‐17‐92 clusters are more commonly found to be associated with endometrial receptivity. Experimental studies show that the targets of the differentially expressed microRNAs affect endometrial receptivity, decidualization, and embryo implantation. Free and exosome/microvesicle containing microRNAs have been detected in human and ovine uterine luminal fluid (ULF). They may serve as mediators of embryo–endometrium dialog. Some observations suggest that the microRNAs in ULF may be used as biomarkers in infertility treatment. Conclusion MicroRNAs in endometrium and blastocysts are involved in the implantation process.

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Section: Mirnas In Endometriummentioning

confidence: 99%

MicroRNA and Embryo Implantation

Liu

Niu

et al. 2015

American J Rep Immunol

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“…MiR-212-3p can regulate the expression of OLFM1 and CTBP1 in human endometrial epithelial cells to enhance spheroid attachment in vitro. Therefore, further studies of the circRNAs of miR-96-5p and miR-212-3p and their associated functions in embryo implantation deserve to be pursued (Kottawatta et al, 2015). Except for miR-96-5p and miR-212-3p, miR-17-5p and miR-20a-5p also contained in the networks (Supporting Information Figure S1c,d).…”

Section: Discussionmentioning

confidence: 99%

Altered expression patterns of circular RNAs between implantation sites and interimplantation sites in early pregnant mice

Zhang

Ding

et al. 2018

Journal Cellular Physiology

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Circular RNAs (circRNAs), a class of newly discovered endogenous noncoding RNAs, have been found to play important roles in regulating gene expression and participate in several biological processes. However, their specific roles in embryo implantation remain unclear. This study aims to investigate the roles of circRNAs in embryo implantation. In the current study, we screened the expression profiles of circRNAs between implantation sites and interimplantation sites in the endometrial tissue of early pregnant mice on Day 5 using microarray assay. Quantitative reverse transcription polymerase chain reaction (qRT‐PCR) of four dysregulated circRNAs was performed to validate the microarray results. Bioinformatics analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and circRNA–microRNA (miRNA)–messenger RNA (mRNA) negative correlation network analyses were performed. The microarray results showed 101 upregulated and 75 downregulated circRNAs (fold change [FC] ≥ 1.5 and p value < 0.05) at implantation sites compared with interimplantation sites. Four randomly selected circRNAs were successfully verified by qRT‐PCR. The GO and KEGG analyses revealed some meaningful terms. Moreover, based on circRNAs microarray data and the miRNA and mRNA high‐throughput data previously published by our groups, differently expressed circRNAs, miRNAs, and mRNAs were performed to conjoint analysis. Then circRNA–miRNA–mRNA negative correlation networks were constructed and 21 downregulated circRNAs, 14 upregulated miRNAs, and 79 downregulated mRNAs were involved in the networks. The findings of this study may provide a novel perspective on circRNAs and lay a foundation for future research into the potential roles of circRNAs in embryo implantation.

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“…For example, miR-137 functions as a tumor suppressor and it can target CtBP1 directly to inhibit EMT (epithelialmesenchymal transition) and induce apoptosis in melanoma cells [39]. Moreover, miR-212 targets CtBP1 in human endometrial epithelial cells to enhance spheroid attachment in vitro [40].…”

Section: Ivyspringmentioning

confidence: 99%

DNA methylation is involved in the pathogenesis of osteoarthritis by regulating CtBP expression and CtBP-mediated signaling

et al. 2020

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Osteoarthritis (OA) is a common type of arthritis. Chronic inflammation is an important contributor to the pathogenesis of OA. The maturation and secretion of proinflammatory cytokines are controlled by inflammasomes, especially NLRP1 (NLR Family Pyrin Domain Containing 1) and NLRP3. In this study, we identified a transactivation mechanism of NLRP3 mediated by CtBPs (C-terminal-binding proteins). We found that both the mRNA and protein levels of CtBPs were significantly increased in OA biopsies. Analyzing the profiles of differentially expressed genes in CtBP-knockdown and overexpression cells, we found that the expression of NLRP3 was dependent on CtBP levels. By the knockdown or overexpression of transcription factors that potentially bind to the promoter of NLRP3, we found that only AP1 could specifically regulate the expression of NLRP3. Using immunoprecipitation (IP) and Co-IP assays, we found that AP1 formed a transcriptional complex with a histone acetyltransferase p300 and CtBPs. The knockdown of any member of this transcriptional complex resulted in a decrease in the expression of NLRP3. To explore the underlying mechanism of CtBP overexpression, we analyzed their promoters and found that they were abundant in CpG islands. Treatment with the DNA methylation inhibitor 5-aza-2′deoxycytidine (AZA) or knockdown of DNMTs (DNA methyltransferases) resulted in the overexpression of CtBPs, while overexpression of DNMTs caused the reverse effects on CtBP expression. Collectively, our results suggest that the decreased DNA methylation levels in the promoters of CtBPs upregulate their expression. Increased CtBPs associated with p300 and AP1 to form a transcriptional complex and activate the expression of NLRP3 and its downstream signaling, eventually aggravating the inflammatory response and leading to the pathogenesis of OA.

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MicroRNA-212 Regulates the Expression of Olfactomedin 1 and C-Terminal Binding Protein 1 in Human Endometrial Epithelial Cells to Enhance Spheroid Attachment In Vitro1

Cited by 24 publications

References 57 publications

MicroRNA and Embryo Implantation

MicroRNA and Embryo Implantation

Altered expression patterns of circular RNAs between implantation sites and interimplantation sites in early pregnant mice

DNA methylation is involved in the pathogenesis of osteoarthritis by regulating CtBP expression and CtBP-mediated signaling

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