MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4

Luο, Xingguang; Tang, Dian; Gao, Tianle; Zhang, Yanliang; Wang, Miao; Quan, Zhengxue; Chen, Jin

doi:10.1159/000369661

Cited by 39 publications

(40 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several studies has demonstrated many miRNAs expression signature associated with tumorigenesis, tumor growth, and metastasis in osteosarcoma [14][15][16]. In the present study, we found that miR-133a was expressed at lower levels in human osteosarcoma cell lines but at a high level in normal human osteoblastic cells.…”

Section: Introductionsupporting

confidence: 60%

“…Thus, miRNAs may potentially serve as molecular targets in cancer therapy. Several studies has demonstrated many miRNAs expression signature associated with cell proliferation, migration, and invasion in osteosarcoma [14][15][16]. Of the various miRNAs, miR-133a has been shown to exert tumor suppressor functions in many types of human cancers, including non-small cell lung cancer [21], gastric cancer [22], colorectal cancer [23], esophageal squamous cell carcinoma [24], ovarian cancer [25], and osteosarcoma [26].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

MicroRNA-133a Inhibits Osteosarcoma Cells Proliferation and Invasion via Targeting IGF-1R

Chen

Fang

Huang

et al. 2016

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression by repressing translation or cleaving RNA transcripts in a sequence-specific manner. Downregulated microRNAs and their roles in cancer development have attracted much attention. A growing body of evidence showed that microRNA-133a (miR-133a) has inhibitory effects on cell proliferation, migration, invasion, and metastasis of osteosarcoma. Methods: MiR-133a expression in human osteosarcoma cell lines and human normal osteoblastic cell line hFOB was investigated by real-time PCR (RT-PCR). The role of miR-133a in human osteosarcoma growth and invasion was assessed in cell lines in vitro and in vivo. Then, luciferase reporter assay validated IGF-1R as a downstream and functional target of miR-133a, and functional studies revealed that the anti-tumor effect of miR-133a was probably due to targeting and repressing of IGF-1R expression. Results: MiR-133a was lower expressed in human osteosarcoma cell lines than human normal osteoblastic cell line hFOB and its effect on inhibiting proliferation, invasion and metastasis is mediated by its direct interaction with the IGF-1R. Furthermore, the tumour-suppressive function of miR-133a probably contributed to inhibiting the activation AKT and ERK signaling pathway. Conclusion: MiR-133a suppresses osteosarcoma progression and metastasis by targeting IGF-1R in human osteosarcoma cells, providing a novel candidate prognostic factor and a potential anti-metastasis therapeutic target in osteosarcoma.

show abstract

Section: Introductionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

MicroRNA-133a Inhibits Osteosarcoma Cells Proliferation and Invasion via Targeting IGF-1R

Chen

Fang

Huang

et al. 2016

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Thus, miRNAs may be potential targets for cancer therapy. A large number of studies reported that many miRNAs are involved in OS cell prolifera tion, migration and invasion (22)(23)(24). To date, the high expression of miR-663b in OS has been revealed however to the best of our knowledge the exact role of miR-663b in OS has not been investigated.…”

Section: Discussionmentioning

confidence: 99%

Blockade of miR-663b inhibits cell proliferation and induces apoptosis in osteosarcoma via regulating TP73 expression

Shu¹,

Ye²,

Gu³

et al. 2018

BLL

View full text Add to dashboard Cite

OBJECTIVE: This study aimed to investigate the exact role of miR-663b in osteosarcoma (OS) progression and further explore the underlying molecular mechanisms. MATERIALS AND METHODS: The miR-663b expression in human OS cell lines was determined by qRT-PCR, and the results suggested that miR-663b was highly expressed in human OS cells. TargetScan was used to predict the potential targets of miR-663b, and the prediction was confi rmed by dual-luciferase reporter assay. To investigate the role of miR-663b in OS, miR-663b was down-regulated in U2OS cells using miR-663b inhibitor. CCK8 and fl ow cytometry were preformed to investigate the proliferation and apoptosis of U2OS cells. Moreover, qRT-PCR and western blot analysis were performed to measure the mRNA and protein expression. RESULTS: We found that miR-663b directly targets TP73 and negatively regulates TP73 expression. MiR-663b inhibitor signifi cantly decreased the proliferation ability of U2OS cells, while the percentage of apoptotic cells was markedly increased. The level of Bcl-2 was notably inhibited by miR-663b inhibitor, while Bax expression was signifi cantly enhanced. Moreover, miR-663b down-regulation promoted p53 and p21 expression in U2OS cells. CONCLUSIONS: MiR-663b down-regulation represses proliferation and induces apoptosis in OS by targeting TP73. Therefore, we provide a potential therapeutic target for OS treatment (Fig. 6, Ref. 27). Text in PDF www.elis.sk.

show abstract

“…In general, miRNAs are composed of 19-25 nucleotides. Science magazine considered the discovery of miRNA to be one of the top ten scientific advancements in 2002and 2003(Garajova et al, 2014Luo et al, 2014). The relationship between miRNAs and tumors was first identified in chronic lymphocytic leukemia (CLL).…”

Section: Discussionmentioning

confidence: 99%

Regulatory role of microRNA184 in osteosarcoma cells

Gr¹,

Wang²,

Xb³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

MicroRNA-212 Inhibits Osteosarcoma Cells Proliferation and Invasion by Down-Regulation of Sox4

Cited by 39 publications

References 26 publications

MicroRNA-133a Inhibits Osteosarcoma Cells Proliferation and Invasion via Targeting IGF-1R

MicroRNA-133a Inhibits Osteosarcoma Cells Proliferation and Invasion via Targeting IGF-1R

Blockade of miR-663b inhibits cell proliferation and induces apoptosis in osteosarcoma via regulating TP73 expression

Regulatory role of microRNA184 in osteosarcoma cells

Contact Info

Product

Resources

About