MicroRNA‐21 regulates the ERK/NF‐κB signaling pathway to affect the proliferation, migration, and apoptosis of human melanoma A375 cells by targeting SPRY1, PDCD4, and PTEN

Abstract

This study aims to explore the effects of microRNA-21 (miR-21) and ERK/NF-κB signaling pathway on human melanoma A375 cells. The melanoma tissues and adjacent normal tissues were obtained from 45 melanoma patients. qRT-PCR was conducted to quantify the expression of miR-21 and the gene mRNA expressions. Human melanoma A375 cells were divided into the Mock, negative control (NC), miR-21 inhibitors, miR-21 inhibitors + siRNA-SPRY1, miR-21 inhibitors + siRNA-PDCD4, and miR-21 inhibitors + siRNA-PTEN groups. Western blotting was used to determine protein expressions. CCK8 assay and Transwell assay were performed to evaluate the proliferation, migration, and invasion of A375 cells. Annexin V/propidium iodide double staining was adopted to detect cell apoptosis. MiR-21 expression was higher in melanoma tissues than in adjacent tissues, while the mRNA and protein expressions of SPRY1, PDCD4, and PTEN were lower in melanoma tissues than in adjacent tissues. Compared with the Mock and NC groups, the miR-21 inhibitors group exhibited increased expressions of SPRY1, PDCD4, and PTEN and decreased expressions of ERK, p-ERK, NF-κB p65, and p-NF-κB p65. After transfection of miR-21 inhibitors, the proliferation, migration, and invasion of A375 cells were inhibited, while the apoptosis of A375 cells was promoted. However, the effects of miR-21 inhibitors on the growth, migration, invasion, and apoptosis of A375 cells were reversed after transfection of siRNA-SPRY1, siRNA-PDCD4, or siRNA-PTEN. MiR-21 can promote the proliferation, migration, and inhibit the apoptosis of human melanoma A375 cells by inhibiting SPRY1, PDCD4, and PTEN via ERK/NF-κB signaling pathway. © 2016 Wiley Periodicals, Inc.

Midazolam inhibits proliferation and accelerates apoptosis of hepatocellular carcinoma cells by elevating microRNA‐124‐3p and suppressing PIM‐1

“…HCC is the third factor causing death that related to the cancers in the world, and also the major reason of mortality in patients with cirrhosis . Moreover, it has been unearthed that the abnormal expression of miRNAs was associated with the development as well as tumorigenesis in human cancers . There was a recent research has proved the expression and prospective functional pathways of miR‐124‐3p in HCC, in which the relationship between miR‐124‐3p and HCC has been ascertained, and our study was designed to explore the combined role of MDZ, miR‐124‐3p, and its target gene PIM‐1 in HCC.…”

“…19 Moreover, it has been unearthed that the abnormal expression of miRNAs was associated with the development as well as tumorigenesis in human cancers. 20 There was a recent research has proved the expression and prospective functional pathways of miR-124-3p in HCC, 21 in which the relationship between miR-124-3p and HCC has been ascertained, and our study was designed to explore the combined role of MDZ, miR-124-3p, and its target gene PIM-1 in HCC. Collectively, we have found that MDZ could mediate miR-124-3p/PIM-1 axis to regulate the molecular mechanisms of malignant phenotype of HCC cells.…”

Exosomal hsa-miR-21-5p derived from growth hormone-secreting pituitary adenoma promotes abnormal bone formation in acromegaly

“…Downregulation of these 2 genes respectively leads to osteoblast proliferation and differentiation. [19][20][21][22][23] In this study, we demonstrated that exosomes produced by GHPA cells can be internalized by osteoblasts, resulting in exosomal miR-21-dependent promotion of osteoblasts proliferation, synthesis of osteocalcin (OCN) and collagen I, and bone formation through a pathway distinct from the traditional GH/IGF-1 axis.…”

Lung Tumour Cell-derived Exosomes Promote the Expansion of MDSC Through miR-21a/PDCD4/IL-6/STAT3 Pathway

“…In our study, we also find it is abundant in several different tumour cell lines (Supplementary 3c, 7c). It is well documented that miR-21a is an oncogenic miRNA to regulate cancer cell proliferation, migration and apoptosis by suppressing the expression of tumour suppressors including PTEN (Mao et al, 2017) and PDCD4 (Asangani et al, 2008;Mao et al, 2017). Recently miR-21a emerges to have a role in regulating inflammatory response and to stimulate production of anti-inflammatory cytokine IL-10 in macrophage through miR-21/PDCD4 axis (Das et al, 2014).…”