MicroRNA-21 facilitates osteoblast activity

Oka, Shogo; Li, Xiaoyan; Zhang, Fengzhu; Tewari, Nitesh; Ma, R.; Zhong, Liangjun; Makishima, Makoto; Liu, Yi; Bhawal, Ujjal K.

doi:10.1016/j.bbrep.2020.100894

Cited by 13 publications

(16 citation statements)

References 37 publications

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“…Moreover, in vitro, Mir-21 acts as an osteogenic promoter of mesenchymal stem cells of the bone marrow and enhances the osteogenic effect of bone marrow stem cells by regulating downstream target protein Sprouty [15]. Other studies have confirmed that Mir-21 is highly expressed in invasive periodontitis tissues and that the activity and number of macrophages in periodontitis tissues are closely positively correlated with the expression level of Mir-21 [16]. These results suggest that the expression level of Mir-21 is closely related to the severity of periodontitis disease [16].…”

Section: Discussionmentioning

confidence: 96%

Strontium ranelate inhibits alveolar bone resorption in diabetic rats with periodontitis by regulating JAK2/STAT3 signaling pathway

Wang¹,

Li²

2023

Trop. J. Pharm Res

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Purpose: To investigate the inhibitory effect of strontium ranelate on alveolar bone resorption in diabetic rats with periodontitis, and the mechanism involved.Methods: Forty-five Sprague-Dawley (SD) rats were allocated to 3 groups, viz, diabetic control, diabetic periodontitis, and diabetic periodontitis + strontium ranelate groups. Diabetes was induced via i.v. administration of streptozotocin (STZ) (45 mg/kg). Then, a rat model of periodontitis was established by ligating the neck of the upper left first molar of each rat with a 0.4-mm orthodontic ligation wire. Strontium ranelate or normal saline was administered by gavage. Four weeks later, various indicators were assessed.Results: There was a significantly higher expression level of Mir-21 in the periodontal tissue of diabetic periodontitis rats than in diabetic control rats. In contrast, this parameter was significantly lower in diabetic periodontitis rats than in periodontal tissues of rats in the diabetic periodontitis + strontium ranelate group, while the translations of JAK2 and STAT3 genes were significantly higher in periodontal tissues of diabetic periodontitis rats (p < 0.05). In contrast, there were lower expression levels of JAK2 and STAT3 in diabetic periodontitis group than in diabetic periodontitis + strontium ranelate group (p < 0.05).Conclusion: Strontium ranelate increases the expression level of miR-21 and activates JAK2/STAT3 signaling pathway, thereby inhibiting the resorption of alveolus bone in rats with diabetes/periodontitis.

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Section: Discussionmentioning

confidence: 96%

Strontium ranelate inhibits alveolar bone resorption in diabetic rats with periodontitis by regulating JAK2/STAT3 signaling pathway

Wang¹,

Li²

2023

Trop. J. Pharm Res

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“…The continuous increase in miR-21 could be explained that miR-21 is a positive regulator of osteoblastic differentiation. Oka et al [ 40 ] showed that miR-21 regulated osteogenic differentiation and mineralization by facilitating the expression of key osteogenic factors, such as alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), Osteopontin (OPN), Osterix (OSX) and Mef2c in MC3T3-E1 cells. Therefore, miR-21 seems to play a major role in osteoblast differentiation and activation during bone regeneration and healing.…”

Section: Discussionmentioning

confidence: 99%

Characterization of circulating molecules and activities in plasma of patients after allogeneic and autologous intraoral bone grafting procedures: a prospective randomized controlled clinical trial in humans

et al. 2022

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Background The objective was to assess whether intraoral bone augmentation procedures have an impact on the patient’s plasma levels of circulating nucleic acids, exosomes, miRNA levels and caspase activities. The null hypothesis was tested, that no significant differences between the two groups will be found. Methods In this prospective randomized controlled clinical trial 35 systemically healthy non-smoking participants were randomly allocated using sealed envelopes by a blinded clinician not involved in the clinical setting. Plasma samples were collected preoperatively and 3 times postoperatively (immediately, 5 weeks and 4 months postoperatively). The test group consisted of twenty-five patients who received allogeneic bone grafting material and the control group of ten patients who received autologous bone grafts. Levels of cell-free DNA (cfDNA) and microRNAs (miR-21, miR-27a, miR-218) were quantified by real-time PCR, caspase activities and exosome concentrations were determined by ELISA. Results Statistical evaluation reveled a significantly higher exosome level before surgery (p = 0.013) and the first postsurgical sample (p = 0.017) in the control group compared to the test group. The levels of miR-27a and miR-218 significantly differed between the plasma samples before surgery and after surgery in both groups. The levels of miR-21 only significantly differed between the pre- and postsurgical plasma samples in the test group, but not in the control group. All patients completed the study, no adverse events were recorded. Conclusions Our data show the diagnostic potential of the plasma levels of miR-27a, miR-218 and miR-21 in detecting changes in bone metabolism after alveolar bone augmentation. Our very promising results indicate that there might be a high diagnostic potential in evaluating the plasma levels of the before mentioned miRNAs in order to detect bone resorption activities before they become clinically relevant. Trial registration Ethical commission of the Ärztekammer Hamburg, Germany (PV5211) on 11/03/2016 as well as by the German Registry of Clinical Studies (DRKS 00,013,010) on 30/07/2018 (http://apps.who.int/trialsearch/).

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“…In MC3T3-E1 cells, icariin attenuated the inhibitory effect of titanium particles on osteoblast differentiation and matrix mineralization by upregulating miR-21-5p (A mature sequence from the 5′ end of the miR-21 stem-loop precursor for the miR-21 guide strand) expression, revealing the promotional role of miR-21-5p in osteoblast differentiation and mineralization ( Lian et al, 2018 ). Oka et al further demonstrated that miR-21 positively regulated osteogenic differentiation and mineralization by promoting the expression of key osteogenic factors ALP, RUNX2, OPN and OSX in MC3T3-E1 cells, and this result was verified in miR-21 knockout mice ( Oka et al, 2021 ). Similarly, in MC3T3-E1 cells, miR-21 promotes osteogenic differentiation by inhibiting the translation of SMAD7 protein ( Li et al, 2015 ).…”

Section: Mir-21 Regulates Osteogenic Differentiationmentioning

confidence: 91%

MicroRNA-21: An Emerging Player in Bone Diseases

Chen

et al. 2021

Front. Pharmacol.

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MicroRNAs (MiRNAs) are small endogenous non-coding RNAs that bind to the 3′-untranslated region of target genes and promote their degradation or inhibit translation, thereby regulating gene expression. MiRNAs are ubiquitous in biology and are involved in many biological processes, playing an important role in a variety of physiological and pathological processes. MiRNA-21 (miR-21) is one of them. In recent years, miR-21 has received a lot of attention from researchers as an emerging player in orthopedic diseases. MiR-21 is closely associated with the occurrence, development, treatment, and prevention of orthopedic diseases through a variety of mechanisms. This review summarizes its effects on osteoblasts, osteoclasts and their relationship with osteoporosis, fracture, osteoarthritis (OA), osteonecrosis, providing a new way of thinking for the diagnosis, treatment and prevention of these bone diseases.

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MicroRNA-21 facilitates osteoblast activity

Cited by 13 publications

References 37 publications

Strontium ranelate inhibits alveolar bone resorption in diabetic rats with periodontitis by regulating JAK2/STAT3 signaling pathway

Strontium ranelate inhibits alveolar bone resorption in diabetic rats with periodontitis by regulating JAK2/STAT3 signaling pathway

Characterization of circulating molecules and activities in plasma of patients after allogeneic and autologous intraoral bone grafting procedures: a prospective randomized controlled clinical trial in humans

MicroRNA-21: An Emerging Player in Bone Diseases

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