MicroRNA-20b-5p regulates propofol-preconditioning-induced inhibition of autophagy in hypoxia-and-reoxygenation-stimulated endothelial cells

Wang, Zhen; Ding, Hui; Song, Wei; Song, Yulong

doi:10.1007/s12038-020-9998-8

Cited by 11 publications

(8 citation statements)

References 26 publications

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“…In contrast, in propofol-preconditioned HUVECs with H/R injury, ULK1 overexpression promotes cell autophagy and apoptosis and restrains the protective effect of miR-20b-5p. 16 In neonatal rat cardiomyocytes and H9c2 cells, it is thought that miR-223 represses hypoxia-induced apoptosis and overactivated autophagy through the Akt/mTOR pathway by targeting PARP-1. 26 ATG proteins are essential for autophagic flux.…”

Section: Main Textmentioning

confidence: 99%

Autophagy in cardiovascular diseases: role of noncoding RNAs

Gao

Chen

Shan

et al. 2021

Molecular Therapy - Nucleic Acids

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Cardiovascular diseases (CVDs) remain the world’s leading cause of death. Cardiomyocyte autophagy helps maintain normal metabolism and functioning of the heart. Importantly, mounting evidence has revealed that autophagy plays a dual role in CVD pathology. Under physiological conditions, moderate autophagy maintains cell metabolic balance by degrading and recycling damaged organelles and proteins, and it promotes myocardial survival, but excessive or insufficient autophagy is equally deleterious and contributes to disease progression. Noncoding RNAs (ncRNAs) are a class of RNAs transcribed from the genome, but most ncRNAs do not code for functional proteins. In recent years, increasingly, various ncRNAs have been identified, and they play important regulatory roles in the physiological and pathological processes of organisms, as well as in autophagy. Thus, determining whether ncRNA-regulated autophagy plays a protective role in CVDs or promotes their progression can help us to develop ncRNAs as therapeutic targets in autophagy-related CVDs. In this review, we briefly summarize the regulatory roles of several important ncRNAs, including microRNAs (miRNAs), long ncRNAs (lncRNAs), and circular RNAs (circRNAs), in the autophagy of various CVDs to provide a theoretical basis for the etiology and pathogenesis of CVDs and develop novel therapies to treat CVDs.

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Section: Main Textmentioning

confidence: 99%

Autophagy in cardiovascular diseases: role of noncoding RNAs

Gao

Chen

Shan

et al. 2021

Molecular Therapy - Nucleic Acids

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“…Previous studies indicate that propofol exerts its protective effects on I/R injury via miRNAs ( 11 , 13 ). Among them, miR-200c is known to promote an oxidative reaction and is associated with diabetic MI/RI ( 14 ).…”

Section: Resultsmentioning

confidence: 99%

“…miRNAs have also been associated with diabetic MI/RI ( 2 ). For example, propofol is suggested to serve as a therapeutic target for MI/RI via interactions with miRNA ( 13 ). One of the most important regulatory factors in diabetic MI/RI is the interacting miRNA family.…”

Section: Introductionmentioning

confidence: 99%

Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury via the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway

Huang

Ding

et al. 2022

Mol Med Rep

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Myocardial ischemia/reperfusion (Mi/ri) syndrome is one of the leading causes of mortality and disability. Propofol postconditioning is known to improve myocardial ischemia/reperfusion injury (Mi/ri). The present study aimed to explore the mechanism of propofol postconditioning in diabetic Mi/ri. diabetic Mi/ri rat models were established and the rats were treated via propofol postconditioning. Staining with 2,3,5-triphenyl-2H-tetrazolium chloride, H&e staining, Tunel staining and eliSa were applied to detect infarct size, pathological changes, apoptosis and oxidative stress-related factor and apoptotic factor levels, respectively. Subsequently, the effect of propofol on H9c2 cells was also assessed using the cell counting Kit-8 assay. High-glucose hypoxia/reperfusion (H/r) models of H9c2 cardiomyocytes were established. mir-200c-3p overexpression or adipor2 silencing combined with propofol postconditioning was performed in H/r-induced H9c2 cells and STaT3 protein expression levels were determined. Propofol postconditioning significantly reduced myocardial infarct size, oxidative stress and apoptosis in diabetic Mi/ri models. Furthermore, propofol postconditioning significantly reduced the oxidative stress and apoptosis of H9c2 cells in high-glucose H/r models. Propofol postconditioning also significantly downregulated miR-200c-3p expression levels and promoted adipor2 expression levels. mir-200c-3p overexpression or AdipoR2 downregulation significantly reversed the effects of propofol postconditioning on its antioxidation and anti-apoptotic effects in H9c2 cells and on decreasing STaT3 phosphorylation levels. Together, the results of the present study demonstrated that propofol postconditioning inhibited mir-200c-3p, upregulated adipor2 and activated the STaT3 signaling pathway, thus alleviating diabetic Mi/ri and therefore highlighting its potential as a treatment of diabetic Mi/ri.

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“…MiR-20b-5p was found to attenuate hypoxia-induced apoptosis in cardiomyocytes [31]. Also, Zhen W et al [32] found the overexpression of miR-20b-5p could increase cell viability and repress autophagy and apoptosis in human umbilical vein endothelial cells with hypoxia-reoxygenation injury. Both hypoxia and hypoxia-reoxygenation models are similar to patients with MI and revascularization of MI, we considered its vital role in regulating CHD.…”

Section: Discussionmentioning

confidence: 97%

Identifying Serum Exosomal-Associated lncRNA/circRNA-miRNA-mRNA Network in Coronary Heart Disease

Jia

Zhou

et al. 2021

Preprint

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Background: Accumulating evidence has indicated that the importance of noncoding RNAs and exosomes in coronary heart disease (CHD). However, the exosomal-associated competing endogenous RNA (ceRNA)-mediated regulatory mechanism in CHD is still unknown. The present study aimed to explore exosomal-associated ceRNA network in CHD.Methods: The dataset, including 6 CHD patients and 32 normal controls, were downloaded from the ExoRBase database. Differentially expressed mRNAs (DEMs), lncRNAs (DELs) and circRNAs (DECs) in the serum exosomes between CHD and normal controls were screened. MicroRNAs (miRNAs) targeting DEMs were predicted by Targetscan and miRanda, miRNAs targeting DELs and DECs were predicted with miRcode and starBase, respectively. The biological functions and related signal pathways of DEMs were analyzed using David and KOBAS database. Subsequently, the protein-protein interaction (PPI) network was established to screen out hub genes, enrichment analyses of hub genes were performed and the ceRNA network (lncRNA/circRNA-miRNA-mRNA) was constructed to elucidate ceRNA axes in CHD.Results: A total of 312 DEMs, 43 DELs and 85 DECs were identified between CHD patients and normal controls. Functional enrichment analysis showed that DEMs were significantly enriched in “chromatin silencing at rDNA”, “telomere organization”, “negative regulation of gene expression, epigenetic”. The PPI network analysis showed that 25 hub DEMs were closely related to CHD, of which, ubiquitin C (UBC) was the most important. The biological function of hub genes showed that they were mainly enriched in “cellular protein metabolic process”. The exosomal-associated ceRNA regulatory network incorporated 48 DEMs, 72 predicted miRNAs, 10 DELs and 15 DECs. LncRNA/circRNA-miRNA-mRNA interaction axes (RPL7AP11/hsa-miR-17-5p/UBC, RPL7AP11/hsa-miR-20b-5p/UBC) were obtained from the network. Conclusions: Our findings have provided a novel perspective on the potential roles of exosomal-associated ceRNA network regulating the pathogenesis of CHD.

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MicroRNA-20b-5p regulates propofol-preconditioning-induced inhibition of autophagy in hypoxia-and-reoxygenation-stimulated endothelial cells

Cited by 11 publications

References 26 publications

Autophagy in cardiovascular diseases: role of noncoding RNAs

Autophagy in cardiovascular diseases: role of noncoding RNAs

Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury via the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway

Identifying Serum Exosomal-Associated lncRNA/circRNA-miRNA-mRNA Network in Coronary Heart Disease

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