microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN

Mao, Jian; Zhou, Rong; Peng, Ai; Liu, Zhi Li; Huang, Shan; Long, Xin; Shu, Yongqian

doi:10.3892/ol.2012.863

Cited by 77 publications

(62 citation statements)

References 37 publications

(40 reference statements)

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“…Reports have claimed that amounts of miRNAs act as tumor suppressor in osteosarcoma. inhibit the osteosarcoma cells migration and invasion by targeting FASN (Mao et al, 2012). Study in Wu X et al revealed that the proliferation of human osteosarcoma was suppressed by downregulating Eag1 .…”

Section: Discussionmentioning

confidence: 99%

miRNA-218 Inhibits Osteosarcoma Cell Migration and Invasion by Down-regulating of TIAM1, MMP2 and MMP9

Jin

Cai²,

Liu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

102

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Deregulated miRNAs participate in osteosarcoma genesis. In this study, the expression of miRNA-218 in human osteosarcomas, adjacent normal tissues and Saos-2 human osteosarcoma cells was first assessed. Then the precise role of miRNA-218 in osteosarcoma cells was investigated. Upon transfection with a miR-218 expression vector, the proliferation of Saos-2 human osteosarcoma cells determined using the ATPlite assay was significantly suppressed, whilw migration of Saos-2 cells detected by wound healing and invasion determined using transwells were dramatically inhibited. Potential target genes of miR-218 were predicted and T-cell lymphoma invasion and metastasis 1 (TIAM1) and matrix metalloproteinase 2 (MMP2) and 9 (MMP9) were identified. This was confirmed by western blotting, which showed that miR-218 expression inhibited TIAM1, MMP2 and MMP9 protein expression. Collectively, these data suggest that miR-218 acts as a tumor suppressor in osteosarcomas by down-regulating TIAM1, MMP2 and MMP9 expression.

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Section: Discussionmentioning

confidence: 99%

miRNA-218 Inhibits Osteosarcoma Cell Migration and Invasion by Down-regulating of TIAM1, MMP2 and MMP9

Jin

Cai²,

Liu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

102

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“…It has been reported that several miRNAs were misregulated in osteosarcoma tissues or cells, such as miR-34, miR-21, miR-140, miR-223 and miR-195 (He et al, 2009;Song et al, 2009;Wu et al, 2011;Mao et al, 2012;Li et al, 2012;Zhao et al, 2013). For instance, expression of miR-34s was decreased in tumor samples, and MiR-34 genes underwent minimal deletions and epigenetic inactivation in osteosarcomas ).…”

Section: Discussionmentioning

confidence: 99%

“…At the molecular level, RECK (reversioninducing-cysteine-rich protein with kazal motifs), a tumor suppressor gene, was a direct target of miR-21 (Wu et al, 2011). Moreover, microRNA-195 was shown to suppress osteosarcoma cell invasion and migration in vitro by targeting fatty acid synthase (FASN) (Mao et al, 2012). Therefore, miRNAs expression has a key role in regulating cellular processes in osteosarcoma, which need to further investigated in the future.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-802 Promotes Osteosarcoma Cell Proliferation by Targeting p27

Cao¹,

Shen²,

Huang³

2013

Asian Pacific Journal of Cancer Prevention

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MicroRNAs have been demonstrated to regulate proliferation and apoptosis in many types of cancers, but biological functions in osteosarcomas remain relatively unknown. Here, we found expression of miR-802 to be up-regulated in osteosarcoma tissues in comparison with adjacent normal tissues. Enforced expression of miR-802 was able to promote cell proliferation in U2OS and MG63 cells, while miR-802 antisense oligonucleotides (antisense miR-802) inhibited cell proliferation. At the molecular level, our results further revealed that expression of p27, a negative cell-cycle regulator, was negatively regulated by miR-802. Therefore, the data reported here indicate that miR-802 is an important regulator in osteosarcoma, our findings contributing to a better understanding of important mis-regulated miRNAs in this tumour type.

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“…Similar observations have been reported in a number of cancer cells. [37][38][39] The expression of CARM1 can be regulated by miR-15a and miR-181 in acute coronary syndrome and human embryonic stem cells reported by two groups, respectively. 22,23 However, no studies have yet examined that CARM1 is a direct target of miR-195-5p in colorectal cancer.…”

Section: Discussionmentioning

confidence: 99%

Coactivator-associated arginine methyltransferase 1 promotes cell growth and is targeted by microRNA-195-5p in human colorectal cancer

Zhang

Gao

et al. 2017

Tumour Biol.

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The pathogenesis of colorectal cancer remains poorly understood. Here, we show that coactivator-associated arginine methyltransferase 1 is frequently upregulated in colorectal cancer tissues and promotes cell growth in vitro and in vivo. Using bioinformatics-based prediction and luciferase reporter system, we found that coactivatorassociated arginine methyltransferase 1 is post-transcriptionally targeted by microRNA-195-5p in colorectal cancer. Ectopic expression of microRNA-195-5p led to the suppression of the coactivator-associated arginine methyltransferase 1 3′-untranslated regions activity and downregulation of the endogenous coactivator-associated arginine methyltransferase 1 protein in colorectal cancer cells. Expression analysis verified that microRNA-195-5p was markedly downregulated in human colorectal cancer tissues, which was negatively correlated with the elevated levels of coactivator-associated arginine methyltransferase 1 protein. Enhanced levels of microRNA-195-5p in colorectal cancer cells resulted in a sharp reduction of cell proliferative and colony-formative capacities in vitro. Remarkably, restoration of coactivator-associated arginine methyltransferase 1 in microRNA-195-5p-transfected colorectal cancer cells partially abrogated the inhibition of cell proliferation and colony formation mediated through microRNA-195-5p. These data confirm that microRNA-195-5p might function as an anti-tumor microRNA in colorectal cancer exerting critical control over coactivator-associated arginine methyltransferase 1 expression. The newly identified microRNA-195-5p/coactivator-associated arginine methyltransferase 1 axis may act as a novel promising therapeutic target for colorectal cancer treatment.

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microRNA-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting FASN

Cited by 77 publications

References 37 publications

miRNA-218 Inhibits Osteosarcoma Cell Migration and Invasion by Down-regulating of TIAM1, MMP2 and MMP9

miRNA-218 Inhibits Osteosarcoma Cell Migration and Invasion by Down-regulating of TIAM1, MMP2 and MMP9

MicroRNA-802 Promotes Osteosarcoma Cell Proliferation by Targeting p27

Coactivator-associated arginine methyltransferase 1 promotes cell growth and is targeted by microRNA-195-5p in human colorectal cancer

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