Coactivator-associated arginine methyltransferase 1 promotes cell growth and is targeted by microRNA-195-5p in human colorectal cancer

Zhang, Meifeng; Wu, Wei; Gao, Ming; Zhang, Jie; Ding, Xinde; Zhu, Ronghua; Chen, Haiqun; Fei, Zhewei

doi:10.1177/1010428317694305

Cited by 9 publications

(5 citation statements)

References 41 publications

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“…To date, deregulated miRNAs and their roles in CRC development have attracted much attention. A series of miRNAs have been reported that miR-19 [6], miR-140-5p [7], miR-149 [8], miR-153 [9], miR-195-5p [10] and miR-301α [11], play important roles in the development of CRC.…”

Section: Introductionmentioning

confidence: 99%

miR-142-3p Suppresses Cell Growth by Targeting CDK4 in Colorectal Cancer

Zhu

Yang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Deregulation of microRNAs (miRNAs) has been associated with a variety of cancers, including colorectal cancer (CRC). Here, we investigated anomalous miR-142-3p expression and its possible functional consequences in primary CRC samples. Methods: The expression of miR-142-3p was measured by quantitative RT-PCR in 116 primary CRC tissues and adjacent non-tumor tissues. The effect of miR-142-3p up- or down-regulation in CRC-derived cells was evaluated in vitro by cell viability and colony formation assays and in vivo by growth assays in xenografted nude mice. Results: Using quantitative RT-PCR, we found that miR-142-3p was down-regulated in 78.4 % (91/116) of the primary CRC tissues tested when compared to the adjacent non-tumor tissues. We also found that the miR-142-3p mimic reduced in vitro cell viability and colony formation by inducing cell cycle arrest in CRC-derived cells, and inhibited in vivo tumor cell growth in xenografted nude mice. Inversely, we found that the miR-142-3p inhibitor increased the viability and colony forming capacity of CRC-derived cells and tumor cell growth in xenografted nude mice. In addition, we identified CDK4 as a potential target of miR-142-3p by predictions and dual-luciferase reporter assays. Concordantly, we found that miR-142-3p mimics and inhibitors could decrease and increase CDK4 protein levels in CRC-derived cells, respectively. Conclusion: From our results we conclude that miR-142-3p may act as a tumor suppressor in CRC and may serve as a tool for miRNA-based CRC therapy.

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Section: Introductionmentioning

confidence: 99%

miR-142-3p Suppresses Cell Growth by Targeting CDK4 in Colorectal Cancer

Zhu

Yang

et al. 2018

Cell Physiol Biochem

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“…Coactivator‐associated arginine methyltransferase 1 ( CARM1 ), the host gene for hsa_circ_0049356, is a type I arginine methyltransferase that is involved in several metabolic activities, including but not limited to autophagy (Wei et al., 2018), epigenetic regulation (Chang et al., 2018) and mRNA decay (Sanchez et al., 2016). Additionally, CARM1 acts as an oncogene in colorectal (Zhang et al., 2017), ovarian (Nakayama et al., 2018) and liver cancers (Zhong et al., 2018). Bao et al found that CARM1 is upregulated in healthy testes as compared to that of the other somatic organs.…”

Section: Discussionmentioning

confidence: 99%

Preliminary investigation of the function of hsa_circ_0049356 in nonobstructive azoospermia patients

Liu

Wen

et al. 2020

Andrologia

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Nonobstructive azoospermia (NOA), which is considered the most severe form of male infertility, has placed a heavy burden on families and society. As vital regulators of transcriptional and post‐transcriptional levels, Noncoding RNAs (ncRNAs) are closely related to all the pathophysiological processes involved in infertility in males, especially spermatogenesis. Our study explored the expression levels of circ_0049356 in both the whole blood and seminal plasma samples of idiopathic NOA patients via quantitative real‐time PCR. Furthermore, the relative expression of its host gene (CARM1) was also determined using the same methods. In addition, as circRNAs have been demonstrated to regulate gene expression as miRNAs sponge, we predicted a total of five miRNAs and 101 mRNAs as putative downstream targets and constructed a circRNA‐miRNA‐mRNA network. Based on the predictions, Gene Ontology and KEGG pathway analyses were performed for further bioinformatics analysis to explore the potential function and investigate the circ_0049356‐miRNA‐mRNA interactions. Our results show target mRNAs that have been predicted to regulate guanyl‐nucleotide exchange factor activity to mediate the GTP/GDP exchange, and downstream targets possibly involved in the regulation of the actin cytoskeleton, which play a significant role in cytoskeleton rearrangement of germ cells during spermatogenesis.

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“…As a mature miRNA, miR-195 was reported to be involved in multiple tumors. It plays a tumor-suppressor role in multiple tumors, including colorectal cancer 8, osteosarcoma 9, breast cancer 10–12, prostate cancer 13,14, in gliomas 15, cervical cancer 16, bladder tumor 17, and hepatocellular carcinoma 18,19 by targeting different target genes. Some studies have reported that miR-195 participates in the chemotherapy resistance of cancer cells.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-195 reverses the resistance to temozolomide through targeting cyclin E1 in glioma cells

Wang¹,

Ren

et al. 2019

Anti-Cancer Drugs

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Glioma is the most common malignant tumor of the central nervous system with poor survival. Temozolomide (TMZ) is the first-line chemotherapy drug for initial and recurrent glioma treatment with a relatively good efficacy, which exerts its antitumor effects mainly through cell death induced by DNA double-strand breaks in the G1 and S phases. However, endogenous or acquired resistance to TMZ limits glioma patients’ clinical outcome and is also an important cause of glioma replase. MicroRNA-195 (miR-195) plays an important role in the regulation of G1-phase/S-phase transition, DNA damage repair, and apoptosis of tumor cells. We found that miR-195 expression was significantly decreased in TMZ-resistant glioma cells induced with TMZ and correlated to the resistance index negatively. Also, the exogenous expression of miR-195 reversed TMZ resistance and induced the apoptosis of TMZ-resistant glioblastoma cells. Further bioinformatics analysis showed cyclin E1 (CCNE1) was a potential target gene of miR-195. Knockdown of CCNE1 partially reversed the effect of decreased miR-195 on TMZ resistance. The data from The Cancer Genome Atlas – Cancer Genome further suggested that hsa-miR-195 could negatively regulate the expression of CCNE1 in glioma. In conclusion, miR-195 reverses the resistance to TMZ by targeting CCNE1 in glioma cells and it could act as a potential target for treatment in glioma with TMZ resistance.

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Coactivator-associated arginine methyltransferase 1 promotes cell growth and is targeted by microRNA-195-5p in human colorectal cancer

Cited by 9 publications

References 41 publications

miR-142-3p Suppresses Cell Growth by Targeting CDK4 in Colorectal Cancer

miR-142-3p Suppresses Cell Growth by Targeting CDK4 in Colorectal Cancer

Preliminary investigation of the function of hsa_circ_0049356 in nonobstructive azoospermia patients

MicroRNA-195 reverses the resistance to temozolomide through targeting cyclin E1 in glioma cells

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