MicroRNA-194 restrains the cell progression of non-small cell lung cancer by targeting human nuclear distribution protein C

Abstract

NSCLC accounts for over 80% of all lung cancers and is associated with poor prognosis. Human nuclear distribution C (hNUDC) was predicted to be the target gene of microRNA-194 (miR-194). The present study was designed to demonstrate the mechanism of miR-194 in the regulation of non-small cell lung cancer (NSCLC) via targeting the hNUDC. The hNUDC expression was found to strongly be increased while the miR-194 decreased significantly in the NSCLC cell lines when compared with the healthy controls. Moreover, the luciferase report confirmed the targeting reaction between miR-194 and hNUDC. After transfection with miR-194 mimic into NSCLC cells, we found that the miR-194 overexpression resulted in abnormal nuclear division, decreased cell proliferation and inhibited the expression of hNUDC and Mpl/ERK pathway proteins. Furthermore, the hNUDC overexpression affected the suppression effect of miR-194 in 95D cells, indicating that miR-194 suppresses tumor cell process by inhibiting the hNUDC expression. In brief, the present study suggests that the upregulation of miR-194 affects the hNUDC expression, leading to a downregulated expression of Mpl/ERK pathway proteins, and suppresses the mitosis and proliferation of NSCLC cells. These results offer a potential therapeutic strategy for the treatment of lung cancer.

Suppression of proliferation, migration and invasion in non-small cell lung cancer cells via profilin 2 inhibition by microRNA-194

“…Currently, there are few studies on the role and underlying mechanism of miR-194 in NSCLC. Zhou et al found that there is relatively low expression of miR-194 in NSCLC patients [20]. After the restoration of miR-194 level in vitro, the proliferation of tumor cells was inhibited, suggesting that the anti-tumor effect of miR-194 may be through the inhibition of its target gene hNUDC [20].…”

“…Zhou et al found that there is relatively low expression of miR-194 in NSCLC patients [20]. After the restoration of miR-194 level in vitro, the proliferation of tumor cells was inhibited, suggesting that the anti-tumor effect of miR-194 may be through the inhibition of its target gene hNUDC [20]. However, due to small sample size, multiple downstream targets and complex regulation network of microRNAs, more studies were warranted to validate the possible target genes of miR-194 and reveal its role in the development of NSCLC.…”

MicroRNA-194 represses glioma cell epithelial-to-mesenchymal transition by targeting Bmi1

“…miR-194 can inhibit colorectal carcinogenesis by targeting mitogen-activated protein kinase 4 (37) or Akt2 (31). Several studies have reported that miR-194 represses the progression and metastasis of non-small lung cancer through targeting bone morphogenetic protein 1 and p27 kip1 (38), forkhead box A1 protein (39) and human nuclear distribution protein C (29). miR-194 can suppress gastric cancer cell proliferation and EMT through targeting RING box protein 1 (40) and forkhead box protein M1 (41).…”

“…Emerging evidence has suggested that miR-194 functions as a tumor suppressor in many types of human cancers including lung cancer (29), hepatocellular carcinoma (30) and colorectal cancer (31). However, the role of miR-194 in glioma remains unclear.…”

MicroRNA-187 modulates epithelial-mesenchymal transition by targeting PTRF in non-small cell lung cancer

“…MicroRNAs (miRNAs), a family of small non-coding RNAs, are processed from precursor RNAs with a typical hairpin secondary structure (8). Several miRNAs are aberrantly expressed in NSCLC and their dysregulation has resulted in cancer progression and poor clinical outcome (9)(10)(11). Considering that miRNAs usually act as EMT-associated downstream effectors of receptor signaling or protein kinases (12), miRNAs may represent novel targets for designing antimetastatic drugs due to their specificity.…”