MicroRNA-194 represses glioma cell epithelial-to-mesenchymal transition by targeting Bmi1

Zhang, Xi; Wei, Chunyan; Jin, Li; Liu, Jiali; Qu, Jianqiang

doi:10.3892/or.2017.5376

Cited by 23 publications

(24 citation statements)

References 70 publications

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“…**P < .01, ***P < .001, compared with the NC group Glioma is the most common and invasive type of major brain tumour, with a high yearly mortality rate worldwide. 19,20 It has now been clearly established that miRNAs have an important role to regulate the tumorigenesis and metastasis of glioma. 21,22 MiR-1275 is an intergenic miRNA, which is encoded by chromosome 6 and associated with tumour progression.…”

Section: Discussionmentioning

confidence: 99%

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Wang

Wen

et al. 2018

J Cellular Molecular Medi

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This study was designed to explore the relationship between miR‐1275 and SERPINE1 and its effects on glioma cell proliferation, migration, invasion and apoptosis. Differentially expressed miRNAs and mRNAs in glioma tissues were screened out by bioinformatic analysis. Dual‐luciferase reporter gene assay was used to validate the targeted relationship between miR‐1275 and SERPINE1. qRT‐PCR was used to detect the expression of miR‐1275 and SERPINE1 in glioma tissues. The expressions of SERPINE1 and p53 pathway‐related proteins in glioma cells were detected by western blot. Glioma cell proliferation, apoptosis, migration and invasion were respectively detected by CCK‐8 assay, flow cytometry, wound healing assay and transwell assay. Tumour xenograft model was developed to study the influence of miR‐1275 and SERPINE1 on glioma growth in vivo. The results of microarray analysis, qRT‐PCR and western blot showed that miR‐1275 was low‐expressed while SERPINE1 was high‐expressed in glioma. Dual‐luciferase assay showed that miR‐1275 could bind to SERPINE1. Overexpression of miR‐1275 could promote the p53 pathway‐related proteins’ expression. Highly expressed miR‐1275 could repress the migration, proliferation and invasion of glioma cells while highly expressed SERPINE1 had inverse effects. Tumour xenograft showed that up‐regulated miR‐1275 or down‐regulated SERPINE1 could repress glioma growth in vivo. Up‐regulation of miR‐1275 activated p53 signalling pathway via regulating SERPINE1 and therefore suppressed glioma cell proliferation, invasion and migration, whereas promoted cell apoptosis.

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Section: Discussionmentioning

confidence: 99%

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Wang

Wen

et al. 2018

J Cellular Molecular Medi

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“…Previous studies have demonstrated that GDNF can promote glioma cell growth by activating MAPK signaling [11, 50, 60], which is consistent with our findings. We found that hsa-miR-152-3p, hsa-miR-194-5p, hsa-miR-629-5p, and hsa-miR-3609b all are able to target MAPK, and hsa-miR-152-3p and hsa-miR-194-5p are both concerned with glioma development [61, 62]. In addition, MAP2K, MAP3K, and ATF2, the key factors in the MAPK signaling pathway, are also target genes of hsa-miR-629-5p, hsa-miR-3609, and hsa-miR-183-5p.…”

Section: Discussionmentioning

confidence: 99%

MiRNAs Mediate GDNF-Induced Proliferation and Migration of Glioma Cells

Zhang

Dong²,

Guo

et al. 2017

Cell Physiol Biochem

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Background/Aims: Glial cell line-derived neurotrophic factor (GDNF) is an important factor promoting invasive glioma growth. This study was performed to reveal a unique mechanism of glioma cell proliferation and migration. Methods: Human U251 glioma cells were used to screen the optimal GDNF concentration and treatment time to stimulate proliferation and migration. MicroRNA (MiRNA) expression profiles were detected by microarray and confirmed by real-time polymerase chain reaction (PCR). The target genes of differentially expressed miRNAs were predicted by miRWalk, and those targeted by multiple miRNAs were screened with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. A regulatory miRNA network was constructed using ingenuity pathway analysis (IPA). Target gene expression of differentially expressed miRNAs was examined by real-time PCR or mRNA microarray. Results: The results show that 50 ng/mL GDNF for 24 h significantly promotes U251 glioma cell proliferation and migration (P < 0.05). Seven miRNAs (hsa-miR-194-5p, hsa-miR-152-3p, hsa-miR-205-5p, hsa-miR-629-5p, hsa-miR-3609, hsa-miR-183-5p, and hsa-miR-487b-3p) were significantly up-regulated after GDNF treatment (P < 0.05). These miRNAs are primarily involved in signal transduction, cell adhesion and cell cycle through mitogen-activated protein kinase (MAPK) signaling, focal adhesion and glioma signal pathways. Five of these miRNAs (hsa-miR-194-5p, hsa-miR-152-3p, hsa-miR-205-5p, hsa-miR-183-5p, and hsa-miR-487b-3p) co-regulate TP53 and Akt. mRNA expression levels of four genes co-targeted by two or more up-regulated miRNAs were significantly decreased after GDNF treatment (P < 0.05). Conclusion: GDNF treatment of U251 glioma cells significantly increased the expression of seven miRNAs involved in cell adhesion and the cell cycle.

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“…42,43 MiR-194-5p has been found to be low expressed in glioma tissue and overexpression of miR-194-5p suppresses invasion and epithelial-mesenchymal transition of glioma cells. 44 Under serumdeprived condition, miR-194-5p was involved in the regulation of endothelial gene expression as well as the functional angiogenic activity. 21 In GECs, we found that miR-194-5p was low expressed.…”

Section: Combined Treatment Of Circ-usp1 and Mir-194-5p Promoted Domentioning

confidence: 99%

Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

Gao

et al. 2019

J Cellular Molecular Medi

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Recent studies indicate circular RNAs are related to dysregulation of vascular endothelial cell function, yet the underlying mechanisms have remained elusive. Here, we characterized the functional role of circular RNA USP1 (circ‐USP1) in the regulation of the blood‐tumour barrier (BTB) permeability and the potential mechanisms. In the current study, the circ‐USP1 expressing level was up‐regulated in glioma cerebral microvascular endothelial cells (GECs) of the BTB model in vitro. Knockdown of circ‐USP1 disrupted the barrier integrity, increased its permeability as well as reduced tight junction‐related protein claudin‐5, occludin and ZO‐1 expressions in GECs. Bioinformatic prediction and luciferase assay indicated that circ‐USP1 bound to miR‐194‐5p and suppressed its activity. MiR‐194‐5p contributed to circ‐USP1 knockdown‐induced increase of BTB permeability via targeting and down‐regulating transcription factor FLI1. Furthermore, FLI1 regulated the expressions of claudin‐5, occludin and ZO‐1 in GECs through binding to their promoter regions. Single or combined treatment of circ‐USP1 and miR‐194‐5p effectively promoted anti‐tumour drug doxorubicin across BTB to induce apoptosis of glioma cells. Overall, this present study identified the crucial regulation of circ‐USP1 on BTB permeability via miR‐194‐5p/FLI1 axis‐mediated regulation of tight junction proteins, which might facilitate the development of therapeutics against human gliomas.

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MicroRNA-194 represses glioma cell epithelial-to-mesenchymal transition by targeting Bmi1

Cited by 23 publications

References 70 publications

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

Retracted: MircoRNA‐1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1

MiRNAs Mediate GDNF-Induced Proliferation and Migration of Glioma Cells

Circular RNA USP1 regulates the permeability of blood‐tumour barrier via miR‐194‐5p/FLI1 axis

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