MicroRNA-194-5p Levels Decrease during Deep Hypothermic Circulatory Arrest

Abstract

Hypothermia has been reported to be effective in protecting the brain in various clinical conditions, including resuscitation after cardiac arrest and complex cardiovascular surgery, and is considered to be a promising therapy for stroke. The present study aimed to confirm the pivotal role that miRNA-194-5p plays in deep hypothermia circulation arrest. On the basis of reductions in expression of miR-194-5p in the circulation of 21 aortic dissection patients who underwent deep hypothermia circulatory arrest, the specific expression, target, and function of miR-194-5p was investigated using primary neuron culture, polymerase chain reaction, in situ hybridization, and flow cytometry methods. Our results showed that miR-194-5p expression was significantly downregulated in hypothermia oxygen glucose deprivation-treated neurons in vitro. Cortical neurons transfected with miR-194-5p mimic exhibited increased death due to oxygen-glucose deprivation. MiR-194-5p mediated the regulation of neuronal death, which involves the downregulation of the specific target protein SUMO2, which is crucial to ischemia tolerance. Collectively, these data highlight the unique role of miR-194-5p in mediating the deep hypothermia circulation arrest response via the regulation of SUMO2. These findings suggest that miR-194-5p could be a potential therapeutic target for intervention in ischemic disease.

Differential expression profiles of circular RNAs in the rat hippocampus after deep hypothermic circulatory arrest

“…19 In vitro studies using oxygen and glucose-deprived primary neuron cultures under hypothermia showed a downregulation of ischemia tolerance-related protein SUMO2 mediated by miR-194-5p. 9 In our study, miR-194-5p was predicted to be…”

“…7 So far, the expression patterns and functions of circRNAs in the brain damage associated with DHCA remain uninvestigated, though previous studies have shown the regulatory role of miRNAs in ischemia-reperfusion, hypothermia, and inflammatory and immune responses after extracorporeal circulation. 8,9 This study aimed to reveal whether circRNAs are implicated in the brain damage after DHCA. A rat model of DHCA was established for identifying differentially expressed circRNAs in the hippocampus.…”

miRNA Changes in Retinal Ganglion Cells after Optic Nerve Crush and Glaucomatous Damage

“…Few studies have been published on the role of miR-194-5p in CNS injury; however, one study corroborates our findings. miR-194-5p delivery into primary cortical neurons, while not directly causative of neuronal cell death, did mediate neuronal death instigated via oxygen-glucose deprivation [ 34 ]. Cortical neurons with enhanced levels of miR-194-5p were exquisitely sensitive to this form of damage.…”

MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock

“…As the expression of miR-194b-5p was significantly downregulated in the serum and small intestine of the weaned piglets, the authors hypothesized that its expression in serum may reflect the decreased expression in the small intestine. This finding is important given that miR-194b-5p has been demonstrated to downregulate the expression of small ubiquitin-like modifier 2 (SUMO2) (140). SUMO2 is pivotal for cell homeostasis during endogenous or environmental stress, including, among others, heat shock and nutrient stress (141).…”