MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock

Miretti, Silvia; Lecchi, Cristina; Ceciliani, Fabrizio; Baratta, Mario

doi:10.3389/fvets.2020.578193

Cited by 46 publications

(38 citation statements)

References 190 publications

(217 reference statements)

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“…This microRNA has been observed ubiquitously in almost all porcine tissues, suggesting its important role in constitutive processes [ 23 , 29 ]. Consequently, its upregulation can have extensive effects on the organism, including its association with stress response in pigs [ 11 ]. Upregulation of ssc-miR-205 expression has been also reported based on microarray experiments in pig serum (among 10 core microRNAs) upon weaning stress [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the specificity and stability of microRNAs represent advantages compared to the measurement of other biomolecules, e.g., proteins or mRNAs [ 8 ]. While in human medicine the research on microRNAs spans from investigating the role of microRNAs in the pathogenesis of multiple diseases, over their utilization as biomarkers to their potential application as therapeutics, research on microRNAs in livestock species is comparably limited [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers

Segura-Wang¹,

Grenier²,

Ilic³

et al. 2021

IJMS

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Deoxynivalenol (DON), a frequent mycotoxin worldwide, impairs human and animal health. The response of microRNAs, small non-coding RNAs, to DON has been scarcely investigated, but holds remarkable potential for biomarker applications. Hence, we aimed to investigate DON-induced changes in the microRNA expression in porcine liver, jejunum and serum by combining targeted and untargeted analyses. Piglets received uncontaminated feed or feed containing 900 µg/kg and 2500 µg/kg DON for four weeks, followed by a wash-out period. In tissue, only slight changes in microRNA expression were detected, with ssc-miR-10b being downregulated in liver of DON-exposed piglets. In serum, several microRNAs were differentially expressed upon DON exposure, four of which were validated by qPCR (ssc-miR-16, ssc-miR-128, ssc-miR-451, ssc-miR-205). The serum microRNA response to DON increased over time and declined after removal of contaminated diets. Receiver operating curve analyses for individual microRNAs were significant, and a combination of the four microRNAs increased the predictive capacity for DON exposure. Predicted microRNA target genes showed enrichment of several pathways including PIK3-AKT, Wnt/β-catenin, and adherens junctions. This study gives, for the first time, a comprehensive view of the porcine microRNA response to DON, providing a basis for future research on microRNAs as biomarkers for mycotoxins.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers

Segura-Wang¹,

Grenier²,

Ilic³

et al. 2021

IJMS

View full text Add to dashboard Cite

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“…In recent times, circulating miRNAs are increasingly considered biomarkers of various diseases and physiological conditions in humans and animals [29][30][31]). Most studies of this type concern the search for biomarkers for various types of cancer, but there are also many reports on biomarkers in Alzheimer's disease [32], atherosclerosis [33] and diabetes [34].…”

Section: Discussionmentioning

confidence: 99%

Consumption of Coconut Oil Affects adipose miRNA Profile in Pigs.

Oczkowicz

Pawlina-Tyszko

Świątkiewicz

et al. 2021

Preprint

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Circulating miRNA molecules are intensively studied for their usefulness as biomarkers of civilization diseases. At the same time, it is known that diet can influence the level of miRNA expression in tissues. Our research aimed to determine how a diet containing various sources of fat (rapeseed oil, beef tallow, coconut oil) and different amounts of cDDGS (corn Dried Distilled Grains with Solubles) affects the miRNA profile in pig fat – the main source of circulating miRNAs. For this purpose, we used Next Generation Sequencing of miRNA libraries. We observed the highest number of differentially expressed miRNAs in the samples from animals that were fed with coconut oil in the diet compared to all other treatments. In contrary, cDDGS appeared to have little effect on miRNA expression. We propose a subset of di-et-related, adipose-specific, conservative miRNAs among mammals, namely: ssc-miR-99b, ssc-miR-4334-3p, ssc-miR-146b, ssc-miR-23a. Moreover, we observed that several miRNAs regulated by dietary fats are considered as biomarkers in human and animal diseases.

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“…By doing so, they are relatively stable even under tough conditions such as long-time storage at room temperature, and low or high pH [10][11][12][13][14]. Aberrant expression of miRNAs is associated with several human and veterinary disorders, including cancer and heart diseases [15][16][17][18][19][20][21][22]. The dysregulation of circulating miRNAs was previously investigated also in MMVD-affected dogs following different approaches, including quantitative real-time PCR (RT-qPCR), microarray, and next-generation sequencing (NGS) [23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Circulating miR-30b-5p is upregulated in Cavalier King Charles Spaniels affected by early myxomatous mitral valve disease

Brambilla

Bagardi

Ghilardi

et al. 2022

Preprint

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There is a growing interest in developing new molecular markers of heart disease in young Cavalier King Charles Spaniels affected by myxomatous mitral valve disease. The aim of the study was to measure the abundance of 3 circulating microRNAs and their application as potential biomarkers in the plasma of Cavalier King Charles Spaniels with early asymptomatic myxomatous mitral valve disease. 33 dogs affected by the disease in American College of Veterinary Internal Medicine (ACVIM) stage B1 were divided in three groups (11 younger than 3 years, 11 older than 3 years and younger than 7 years, and 11 older than 7 years), and 11 healthy (ACVIM stage A) Cavalier King Charles Spaniels were included as the control group. This is a prospective cross-sectional study. The abundance of three circulating microRNAs (miR-1-3p, miR30b-5p, and miR-128-3p) was measured by quantitative real-time PCR using TaqMan® probes. Diagnostic performance was evaluated by calculating the area under the receiver operating curve (AUC). miR-30b-5p was significantly higher in ACVIM B1 dogs compared to ACVIM A subjects, and the area under the receiver operating curve was 0.79. According to the age of dogs, the abundance of miR-30b-5p was statistically significantly higher in group B1<3y (2.3 folds, P = 0.034), B1 3-7y (2.2 folds, P = 0.028), and B1>7y (2.7 folds, P = 0.018) than in group A. The area under the receiver operating curves were fair in discriminating between group B1<3y and group A (AUC 0.780), between B1 3-7y and A (AUC 0.78), and good in discriminating between group B1>7y and A (AUC 0.822). miR-30b-5p changed in the plasma of dogs at the asymptomatic stage of disease, particularly at a young age.

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MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock

Cited by 46 publications

References 190 publications

MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers

MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers

Consumption of Coconut Oil Affects adipose miRNA Profile in Pigs.

Circulating miR-30b-5p is upregulated in Cavalier King Charles Spaniels affected by early myxomatous mitral valve disease

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