MicroRNA‐188 regulates aging‐associated metabolic phenotype

Abstract

With the increasing aging population, aging‐associated diseases are becoming epidemic worldwide, including aging‐associated metabolic dysfunction. However, the underlying mechanisms are poorly understood. In the present study, we aimed to investigate the role of microRNA miR‐188 in the aging‐associated metabolic phenotype. The results showed that the expression of miR‐188 increased gradually in brown adipose tissue (BAT) and inguinal white adipose tissue (iWAT) of mice during aging. MiR‐188 knockout mice were resistant to the aging‐associated metabolic phenotype and had higher energy expenditure. Meanwhile, adipose tissue‐specific miR‐188 transgenic mice displayed the opposite phenotype. Mechanistically, we identified the thermogenic‐related gene Prdm16 (encoding PR domain containing 16) as the direct target of miR‐188. Notably, inhibition of miR‐188 expression in BAT and iWAT of aged mice by tail vein injection of antagomiR‐188 ameliorated aging‐associated metabolic dysfunction significantly. Taken together, our findings suggested that miR‐188 plays an important role in the regulation of the aging‐associated metabolic phenotype, and targeting miR‐188 could be an effective strategy to prevent aging‐associated metabolic dysfunction.

Positive Effect of Gushukang on Type-H Vessel and Bone Formation

“…These results suggest that bone anabolism is greater than bone catabolism after GSK treatment, confirming that GSK enhances bone density and bone mass. Tartrate-resistant acid phosphatase, produced by osteoclasts, and OCN, secreted by osteoblasts (Booth et al, 2016;Calvier et al, 2017), levels reflect the activity of osteoclasts and osteoblasts as well as the status of bone resorption and bone formation (Long et al, 2014;Yu et al, 2015;Huang et al, 2020). In our study, TRAP and OCN staining results suggest that GSK treatment increased bone mass by activating osteoblasts and suppressing osteoclasts.…”

Obesity and Bone Health: A Complex Link

“…BAT, however, is an energy-consuming organization that is involved in non-shivering heat and diet-induced heat production. Both thermogenic mechanisms are due to the exclusive expression of uncoupled protein-1 (UCPl) in the mitochondria of brown fat cells, resulting in the uncoupling of fatty acids and ATP oxidation, ultimately causing energy dissipation in the form of heat ( Del Mar Gonzalez-Barroso et al, 2001 ; Carobbio et al, 2017 ; Huang et al, 2020 ). Thus, BAT can be capable of fighting obesity.…”

Communications Between Bone Marrow Macrophages and Bone Cells in Bone Remodeling

“…Aging is usually accompanied by an irreversible recession in physiological functions. Age-associated metabolic dysfunction includes, but is not limited to, increased fat mass accumulation and insulin sensitivity deterioration (Huang et al, 2020).…”

“…Aging is usually accompanied by an irreversible recession in physiological functions. Age-associated metabolic dysfunction includes, but is not limited to, increased fat mass accumulation and insulin sensitivity deterioration (Huang et al, 2020 ). Aging is usually accompanied by a decrease in M2-type macrophages and an increase in proinflammatory factors, which promote the M1 transformation of the macrophage phenotype to aggravate the symptom.…”