MicroRNA-184 inhibits cell proliferation and metastasis in human colorectal cancer by directly targeting IGF-1R

Wu, Guannan; Liu, Jiayun; Wu, Zhenfeng; Wu, Xiaoyu; Yao, Xuequan

doi:10.3892/ol.2017.6499

Cited by 30 publications

(25 citation statements)

References 47 publications

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“…In this study, after bioinformatics analysis validation of IGF‐1R as a potential target for miR‐184, miRNA mimic significantly decreased the IGF‐1R protein level in HCT‐116 CRC cell line, this decrease was observed in the SW‐620 cell line even more than HCT‐116. According to this study, reduction in IGF‐1R level effectively inhibited proliferation, migration, and cellular invasion in CRC (Wu et al, ).…”

Section: Discussionmentioning

confidence: 88%

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Let‐7e enhances the radiosensitivity of colorectal cancer cells by directly targeting insulin‐like growth factor 1 receptor

Samadi

Afshar

Amini

et al. 2018

Journal Cellular Physiology

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Abnormal expression of various microRNAs (miRNAs), as regulators of biological signaling pathways, has a strong association with cancer resistance to chemotherapy and radiotherapy. The let‐7 family of miRNAs as tumor suppressors have shown to be downregulated in different types of human malignancies including colorectal cancer (CRC). However, the biological function of let‐7 members in the processes of resistance to radiation in CRC has not yet been completely elucidated. Insulin‐like growth factor 1 receptor (IGF‐1R) signaling pathway is amplified in CRC and leads to its progression, development, and also radiation resistance. So, it seems like an attractive target for anticancer therapy. In this study, by using bioinformatics analysis, it has been revealed that IGF‐1R is a direct target of the let‐7e member. Consistent with this, we identified that increased levels of let‐7e in CRC cells reduced IGF‐1R protein level and subsequently its downstream signaling pathways, which resulted in the G1 cell cycle arrest and a significant reduction in the proliferation, survival and also resistance to radiation of CRC cells. Altogether, these results suggested that let‐7e by targeting the IGF‐1R signaling pathway might serve as therapeutics in anticancer therapy.

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Section: Discussionmentioning

confidence: 88%

“…Hence, blocking the IGF‐1R pathways and subsequent inhibition of involving proteins and regulatory factors could reduce cancer survival, invasion, metastasis, and also resistance to radiation therapy. In another study by Wu, Liu, Wu, Wu, and Yao (). it has been showed that miR‐184 is a tumor suppressor miRNA in CRC.…”

Section: Discussionmentioning

confidence: 97%

Let‐7e enhances the radiosensitivity of colorectal cancer cells by directly targeting insulin‐like growth factor 1 receptor

Samadi

Afshar

Amini

et al. 2018

Journal Cellular Physiology

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“…[14]. In human colorectal cancer, miR-184 was downregulated in CRC tissues and cell lines, which significantly inhibited proliferation, migration and invasion in human colorectal cancer by directly targeting IGF-1R [26]. However, some studies have suggested miR-184 as a potential onco-miR in various human tumours.…”

Section: Mir-184 Inhibits Npc Metastasis By Decreasing Emt In Vivomentioning

confidence: 99%

miR-184 Inhibits Tumor Invasion, Migration and Metastasis in Nasopharyngeal Carcinoma by Targeting Notch2

Zhu¹,

Jiang

et al. 2018

Cell Physiol Biochem

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Background/Aims: A recent study found that dysregulated microRNA-184 (miR-184) is involved in the proliferation and survival of nasopharyngeal carcinoma (NPC). This study aimed to evaluate the detailed mechanisms of invasion, migration and metastasis of NPC cells. Methods: Quantitative reverse-transcription PCR (qRT-PCR) and Western blot were used to confirm the expression levels of miR-184 and Notch2. NPC cell invasion and migration were subsequently examined using in vitro cell invasion and wound-healing assays, respectively. MicroRNA (miRNA) target gene prediction databases and dual-luciferase reporter assay were adopted to validate the target genes of miR-184. Results: MiR-184 was downregulated in the NPC cell lines. The miR-184 inhibitor increased the number of invading NPC cells, whereas miR-184 mimics inhibited the invasive ability of such cells. The protein level of E-cadherin decreased, whereas those of N-cadherin and vimentin increased in the anti-miR-184 group. This result showed that miR-184 inhibited NPC cell invasion and metastasis by regulating EMT progression. MiRNA target gene prediction databases indicated the potential of Notch2 as a direct target gene of miR-184. Such a notion was then validated by results of dual-luciferase reporter assay. Notably, shRNANotch2 restrained the EMT and partially abrogated the inhibitory effects of miR-184 on EMT progression in NPC cells. Conclusion: MiR-184 functions as a tumour-suppressive miRNA targeting Notch2 and inhibits the invasion, migration and metastasis of NPC.

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“…Ma et al, ). Another two upregulated miRNAs, which are also known as tumor suppressors are miR‐184 and miR‐874.MiR‐184, a highly conserved miRNA, has been identified as an inhibitor of cell proliferation in human colorectal cancer by directly targeting IGF1R (Wu, Liu, Wu, Wu, & Yao, ). In addition, it was assumed that miR‐184 played a role in various steps of oogenesis and early embryonic development in Drosophila (Iovino, Pane, & Gaul, ).…”

Section: Discussionmentioning

confidence: 99%

microRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles

Gad

Němcová

Murín

et al. 2019

Molecular Reproduction Devel

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Oocyte developmental competence is acquired during folliculogenesis and regulated by complex molecular mechanisms. Several molecules are involved in these mechanisms, including microRNAs (miRNAs) that are essential for oocyte‐specific processes throughout the development. The objective of this study was to identify the expression profile of miRNAs in porcine oocytes derived from follicles of different sizes using RNA deep sequencing. Oocytes were aspirated from large (LO; 3–6 mm) or small (SO; 1.5–1.9 mm) follicles and tested for developmental competence and chromatin configurations. Small RNA libraries were constructed from both groups and then sequenced in an Illumina NextSeq. 500. Oocytes from the LO group exhibited higher developmental competence and different chromatin configuration compared with oocytes from the SO group. In total, 167 and 162 known miRNAs were detected in the LO and SO groups, respectively. MiR‐205, miR‐16, miR‐148a‐3p, and miR‐125b were among the top 10 highly expressed miRNAs in both groups. Eight miRNAs were differentially expressed (DE) between both groups. Target gene prediction and pathway analysis revealed 46 pathways that were enriched with miRNA‐target genes. The oocyte meiosis pathway and signaling pathways including FoxO, PI3K‐Akt, and cAMP were predictably targeted by DE miRNAs. These results give more insights into the potential role of miRNAs in regulating the oocyte development.

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MicroRNA-184 inhibits cell proliferation and metastasis in human colorectal cancer by directly targeting IGF-1R

Cited by 30 publications

References 47 publications

Let‐7e enhances the radiosensitivity of colorectal cancer cells by directly targeting insulin‐like growth factor 1 receptor

Let‐7e enhances the radiosensitivity of colorectal cancer cells by directly targeting insulin‐like growth factor 1 receptor

miR-184 Inhibits Tumor Invasion, Migration and Metastasis in Nasopharyngeal Carcinoma by Targeting Notch2

microRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles

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