MicroRNA-151a-3p Functions in the Regulation of Osteoclast Differentiation: Significance to Postmenopausal Osteoporosis

Abstract

Background: Studies have found the pivotal role of miRNAs in the progression of postmenopausal osteoporosis (OP). However, the function of miRNAs in OP is unclear. This study aimed to explore the biological functions of microRNA-151a-3p in OP. Methods: RT-qPCR was employed to assess the expression of microRNA-151a-3p in serum isolated from OP patients and healthy controls. Dual-energy X-ray absorptiometry (DXA) was used to measure the bone mineral density (BMD) of the lumbar spine. The expression levels of c-Fos, NFATc1, and TRAP were tested by Western blot. Ovariectomized (OVX) rats were treated with antago microRNA-151a-3p or antago NC, and then serum and lumbar vertebrae were collected for ELISA and bone histomorphology analysis. Results: The expression of microRNA-151a-3p in postmenopausal women with osteoporosis was significantly up-regulated, and microRNA-151a-3p level was negatively correlated with BMD. During osteoclastogenesis, microRNA-151a-3p level was obviously increased. Overexpression of microRNA-151a-3p promoted the differentiation of RANKL-induced THP-1 and RAW264.7 cells into osteoclasts, whereas silencing of microRNA-151a-3p resulted in the opposite results. Silencing of microRNA-151a-3p in OVX rats altered osteoclastogenesis-related factors and raised BMD. Conclusion:MicroRNA-151a-3p could partly regulate osteoporosis by promoting osteoclast differentiation, and miRNA-151a-3p could be a potential therapeutic target for postmenopausal osteoporosis.

MicroRNA Regulation of Bone Marrow Mesenchymal Stem Cells in the Development of Osteoporosis in Obesity

“…The expression of miR-199b-5p, miR-10a-5p, miR-10b-5p, miR-24-1-5p, miR-223-3p, and miR-338-3p was suppressed, whereas miR-291a-5p, miR-125b-1-3p, and miR-291b-5p expression was enhanced (Table 3, Ref. [100][101][102][103][104][105][106][107][108]) (Fig. 2).…”

“…In women with postmenopausal osteoporosis, the levels of miR-133a and miR-151a-3p were increased in blood serum and negatively correlated with bone mineral density [104,105] (Table 3). The overexpression of miR-133a and miRNA-151a-3p activated the RANKL-induced differentiation of RAW264.7 and THP-1 cells (murine monocyte/macrophage lines) into osteoclasts (Fig.…”

Effects of Pamidronate Disodium Combined with Calcium on BMD Values and Severity of Pain in Elderly Patients with Osteoporosis Based on Mobile Terminal Platform for Internet of Things

“…(4) Patients had not taken any drugs affecting the bone metabolism in the last three months. (5) The age of patients was more than 70 years old. ( 6) All patients were treated in our hospital during the whole process with the complete clinical information.…”

“…Exclusion criteria were as follows: (1) patients with secondary osteoporosis due to the endocrine metabolism, alimentary deficiency, and rheumatism; (2) patients who had taken drugs that might affect the bone metabolism in the last three months; (3) patients with the history of malignant tumor and cardiovascular disease or patients with the diseases that could affect the bone metabolism; (4) patients with the irrational drug use that might have the undefined treatment effects; (5) patients with the incomplete clinical information; (6) patients without complete follow-up; and (7) patients with psychiatric disorders and who could not communicate with others.…”

“…Therefore, the deepening of clinical research on osteoporosis is beneficial for reducing the future medical burden in China and improving the prognosis of elderly patients with osteoporosis. At present, calcium is the main medicine for the treatment of osteoporosis, but the calcium treatment alone cannot exert an ideal prevention and control effect, and elderly patients with low calcium absorption rate in intestinal tract and 1 α -hydrolase activity of kidney cannot benefit from it [ 5 , 6 ]. In addition to calcium, bisphosphonates are also the first-line drug for the treatment of osteoporosis, which can inhibit the bone remodeling, increase the BMD, improve the bone microarchitecture of patients, and reduce the possibility of fracture [ 7 ].…”