MicroRNA-133a regulates the mRNAs of two invadopodia-related proteins, FSCN1 and MMP14, in esophageal cancer

Akanuma, Naoki; Hoshino, Isamu; Akutsu, Yasunori; Murakami, Kentaro; Isozaki, Yuka; Maruyama, Tetsuro; Yusup, Gulbostan; Qin, Wei; Toyozumi, Takeshi; Takahashi, Masahīko; Suito, Hiroshi; Hu, Xinyang; Sekino, N; Matsubara, Hisahiro

doi:10.1038/bjc.2013.676

Cited by 97 publications

(85 citation statements)

References 35 publications

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“…In addition, the association between miR-133a and FSCN1 has been reported in several other types of cancer, including esophageal carcinoma, breast cancer and bladder cancer (13,(24)(25)(26). For example, Akanuma et al (13) observed that the mRNA level of FSCN1 was upregulated in esophageal squamous cell carcinoma tissues and inversely correlated with the expression level of miR-133a. The authors further demonstrated that miR-133a inhibited cell proliferation and invasion, partly as least, via inhibition of FSCN1 in esophageal squamous cell carcinoma cells.…”

Section: A B Cmentioning

confidence: 96%

MicroRNA-133a inhibits proliferation and invasion, and induces apoptosis in gastric carcinoma cells via targeting fascin actin-bundling protein 1

Chen

2012

Molecular Medicine Reports

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Abstract. Fascin actin-bundling protein 1 (FSCN1) is associated with tumor progression. In addition, deregulation of the expression of FSCN1 has been observed in certain types of cancer. However, the detailed role of FSCN1 in gastric cancer remains to be elucidated. In the present study, downregulation of microRNA (miR)-133a and upregulation of FSCN1 were both observed in gastric cancer tissues and cell lines. Functional studies have revealed that miR-133a is able to bind to the 3'-untranslated region of FSCN1 mRNA, and overexpression of miR-133a causes downregulation of FSCN1 expression, while downregulation of miR-133a leads to an increased FSCN1 expression in gastric cancer cells. Furthermore, overexpression of miR-133a inhibited proliferation and invasion, but promoted apoptosis of gastric cancer cells, which may be reversed by upregulation of FSCN1. By contrast, downregulation of miR-133a enhanced proliferation and invasion, but suppressed apoptosis in gastric cancer cells. In conclusion, the anti-oncogenic activity of miR-133a may involve the inhibition of the target gene FSCN1. The present study suggested that miR-133a may be a potential therapeutic target in the treatment of gastric cancer.

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Section: A B Cmentioning

confidence: 96%

MicroRNA-133a inhibits proliferation and invasion, and induces apoptosis in gastric carcinoma cells via targeting fascin actin-bundling protein 1

Chen

2012

Molecular Medicine Reports

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“…Several studies has demonstrated many miRNAs expression signature associated with cell proliferation, migration, and invasion in osteosarcoma [14][15][16]. Of the various miRNAs, miR-133a has been shown to exert tumor suppressor functions in many types of human cancers, including non-small cell lung cancer [21], gastric cancer [22], colorectal cancer [23], esophageal squamous cell carcinoma [24], ovarian cancer [25], and osteosarcoma [26]. Fujiwara et al [27] found that down-regulation of miR-133a was inversely associated with the overall survival of osteosarcoma patients, suggesting that miR-133a potentially inhibits osteosarcoma development.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-133a Inhibits Osteosarcoma Cells Proliferation and Invasion via Targeting IGF-1R

Chen

Fang

Huang

et al. 2016

Cell Physiol Biochem

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Background/Aims: MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression by repressing translation or cleaving RNA transcripts in a sequence-specific manner. Downregulated microRNAs and their roles in cancer development have attracted much attention. A growing body of evidence showed that microRNA-133a (miR-133a) has inhibitory effects on cell proliferation, migration, invasion, and metastasis of osteosarcoma. Methods: MiR-133a expression in human osteosarcoma cell lines and human normal osteoblastic cell line hFOB was investigated by real-time PCR (RT-PCR). The role of miR-133a in human osteosarcoma growth and invasion was assessed in cell lines in vitro and in vivo. Then, luciferase reporter assay validated IGF-1R as a downstream and functional target of miR-133a, and functional studies revealed that the anti-tumor effect of miR-133a was probably due to targeting and repressing of IGF-1R expression. Results: MiR-133a was lower expressed in human osteosarcoma cell lines than human normal osteoblastic cell line hFOB and its effect on inhibiting proliferation, invasion and metastasis is mediated by its direct interaction with the IGF-1R. Furthermore, the tumour-suppressive function of miR-133a probably contributed to inhibiting the activation AKT and ERK signaling pathway. Conclusion: MiR-133a suppresses osteosarcoma progression and metastasis by targeting IGF-1R in human osteosarcoma cells, providing a novel candidate prognostic factor and a potential anti-metastasis therapeutic target in osteosarcoma.

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“…The literature reveals that the expression of FSCN1 is an independent poor prognostic factor according to a multivariate analysis, and co-expression with MMP14 correlates with the poorest overall survival in esophageal cancer. The knockdown of FSCN1 inhibits the proliferation and invasion of ESCC cells (16). Chen et al (17) reported that FSCN1 was overexpressed in colorectal cancers, and was associated with cancer cell progression.…”

Section: Discussionmentioning

confidence: 99%

Identification of candidate target genes of genomic aberrations in esophageal squamous cell carcinoma

et al. 2016

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Abstract. The aim of the present study was to identify the candidate target genes of genomic aberrations in esophageal squamous cell carcinoma (ESCC). Array comparative genomic hybridization (CGH) and quantitative polymerase chain reaction were applied to analyze the copy number changes and expression level of candidate genes, respectively. Integrative analysis revealed that homozygous deletions of cyclin-dependent kinase inhibitor (CDKN) 2A and CDKN2B and gains of fascin actin-bundling protein 1 (FSCN1) and homer scaffolding protein 3 (HOMER3) occurred frequently in ESCC. The results demonstrated that the homozygous deletion of CDKN2A or CDKN2B was significantly associated with lymph node metastasis. Notably, the expression of CDKN2A and CDKN2B was lower in dysplasia than in normal esophageal epithelium. We also observed that the copy number increase of FSCN1 was significantly associated with pT, pN and pStage, and that the gain of HOMER3 was significantly linked with pN and pStage. We further revealed that FSCN1 and HOMER3 were overexpressed in ESCC, and that their overexpression was correlated with copy number increase. In conclusion, CDKN2A, CDKN2B, FSCN1 and HOMER3 are candidate cancer-associated genes and may play a tumorigenic role in ESCC.

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MicroRNA-133a regulates the mRNAs of two invadopodia-related proteins, FSCN1 and MMP14, in esophageal cancer

Cited by 97 publications

References 35 publications

MicroRNA-133a inhibits proliferation and invasion, and induces apoptosis in gastric carcinoma cells via targeting fascin actin-bundling protein 1

MicroRNA-133a inhibits proliferation and invasion, and induces apoptosis in gastric carcinoma cells via targeting fascin actin-bundling protein 1

MicroRNA-133a Inhibits Osteosarcoma Cells Proliferation and Invasion via Targeting IGF-1R

Identification of candidate target genes of genomic aberrations in esophageal squamous cell carcinoma

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