MicroRNA-125a-3p participates in odontoblastic differentiation of dental pulp stem cells by targeting Fyn

Wang, Jihua; Zheng, Ya; Bai, Bingbing; Song, Yihua; Zheng, Ke; Xiao, Jinwen; Lv, Yi; Bao, Liuliu; Zhou, Qiao; Ji, Lujun; Feng, Xiaoxing

doi:10.1007/s10616-019-00358-7

Cited by 19 publications

(14 citation statements)

References 29 publications

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“…Besides, DPSCs treated with TNF- α exhibit increased expression of Fyn, a member of the protein tyrosine kinase Src family, which is related to inflammation and odontogenesis; however, the expression of miR-125a-3p is decreased. It has been found that miR-125-3p can reverse the inflammatory response and enhance odontogenic differentiation by repressing Fyn [ 169 ]. Moreover, a certain concentration of LPS can improve the proliferation, adhesion, and migration of DPSCs and differentiation of odontoblast through Toll-like receptor (TLR-4), ERK, and P38 MAPK signaling pathways [ 164 , 170 ].…”

Section: Epigenetic Mechanisms In Dpscsmentioning

confidence: 99%

Epigenetic Regulation of Dental Pulp Stem Cell Fate

Zhou

Gan

Yan

et al. 2020

Stem Cells International

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Epigenetic regulation, mainly involving DNA methylation, histone modification, and noncoding RNAs, affects gene expression without modifying the primary DNA sequence and modulates cell fate. Mesenchymal stem cells derived from dental pulp, also called dental pulp stem cells (DPSCs), exhibit multipotent differentiation capacity and can promote various biological processes, including odontogenesis, osteogenesis, angiogenesis, myogenesis, and chondrogenesis. Over the past decades, increased attention has been attracted by the use of DPSCs in the field of regenerative medicine. According to a series of studies, epigenetic regulation is essential for DPSCs to differentiate into specialized cells. In this review, we summarize the mechanisms involved in the epigenetic regulation of the fate of DPSCs.

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Section: Epigenetic Mechanisms In Dpscsmentioning

confidence: 99%

Epigenetic Regulation of Dental Pulp Stem Cell Fate

Zhou

Gan

Yan

et al. 2020

Stem Cells International

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“…Increasing research and evidence have proved that anomalous levels of miRNAs are closely associated with the genesis and progression of different types of diseases, and they further regulate the biological function of various cells, including hDPSCs ( 11 , 12 ). For example, miR-125a-3p is found to have the ability to regulate the odontoblastic differentiation of hDPSCs by targeting Fyn ( 13 ). Low-expression of miR-224-5p could enhance the migration and proliferation of hDPSCs ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

Huang

2021

IJSC

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Background and Objectives MiR-148a-3p has been reported to regulate the differentiation of marrow stromal cell osteoblast. In this study, whether miR-148a-3p regulated the odontoblastic differentiation of human dental pulp stem cells (hDPSCs) or not was explored. Methods and Results The hDPSCs were isolated and identified via flow cytometry. Targets of miR-148a-3p were identified via bioinformatics and dual-luciferase reporter assay. After the cell was cultured in the odontogenic differentiation medium or infected, cell viability, invasion, and odontoblastic differentiation were detected via MTT, transwell, and Alizarin Red S staining, respectively. The miR-148a-3p, Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions were determined by RT-qPCR and Western blot. The hDPSCs odontoblastic differentiation downregulated the miR-148a-3p expression and upregulated Wnt1 expression. Wnt1 was determined as the target for miR-148a-3p. MiR-148a-3p mimic and siWnt1 suppressed the cell viability, invasion, and odontoblastic differentiation of hDPSCs and inhibited the Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions. In contrast, miR-148a-3p inhibitor and overexpressed Wnt1 promoted the cell viability, invasion, and odontoblastic differentiation of hDPSCs, and upregulated the Wnt1, β-catenin, DSPP, DMP-1, RUNX2, OCN, and Smad4 expressions. Also, miR-148a-3p mimic and inhibitor reversed the effects of Wnt1 overexpression and siWnt1. Conclusions MiR-148a-3p modulated the invasion and odontoblastic differentiation of hDPSCs through the Wnt1/β-catenin pathway.

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“…Fyn is a member of the protein tyrosine kinase Src family, and its overexpression is associated with inflammation and odontogenesis [94]. These findings suggest that the induction of miR-125b overexpression in pulpal inflammation can reverse the inflammatory response and improve odontogenic differentiation, through the repression of Fyn [94,136].…”

Section: Mir-125bmentioning

confidence: 94%

“…About the role of miR-125a and miR-125b in pulp inflammation, to date, there is no evidence of miR-125b. Regarding miR-125a, there is only one study in which it was observed that the stimulation of DPSCs with TNF-α induces the downregulation of the expression of miR-125b, while the expression of Fyn was upregulated [136]. Fyn is a member of the protein tyrosine kinase Src family, and its overexpression is associated with inflammation and odontogenesis [94].…”

Section: Mir-125bmentioning

confidence: 99%

The Role of microRNAs in Pulp Inflammation

Muñoz‐Carrillo

Vázquez-Alcaraz

Vargas-Barbosa

et al. 2021

Cells

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The dental pulp can be affected by thermal, physical, chemical, and bacterial phenomena that stimulate the inflammatory response. The pulp tissue produces an immunological, cellular, and vascular reaction in an attempt to defend itself and resolve the affected tissue. The expression of different microRNAs during pulp inflammation has been previously documented. MicroRNAs (miRNAs) are endogenous small molecules involved in the transcription of genes that regulate the immune system and the inflammatory response. They are present in cellular and physiological functions, as well as in the pathogenesis of human diseases, becoming potential biomarkers for diagnosis, prognosis, monitoring, and safety. Previous studies have evidenced the different roles played by miRNAs in proinflammatory, anti-inflammatory, and immunological phenomena in the dental pulp, highlighting specific key functions of pulp pathology. This systematized review aims to provide an understanding of the role of the different microRNAs detected in the pulp and their effects on the expression of the different target genes that are involved during pulp inflammation.

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MicroRNA-125a-3p participates in odontoblastic differentiation of dental pulp stem cells by targeting Fyn

Cited by 19 publications

References 29 publications

Epigenetic Regulation of Dental Pulp Stem Cell Fate

Epigenetic Regulation of Dental Pulp Stem Cell Fate

MiR-148a-3p Regulates the Invasion and Odontoblastic Differentiation of Human Dental Pulp Stem Cells via the Wnt1/β-Catenin Pathway

The Role of microRNAs in Pulp Inflammation

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