MicroRNA-124 Reduces the Pentose Phosphate Pathway and Proliferation by Targeting PRPS1 and RPIA mRNAs in Human Colorectal Cancer Cells

Qiu, Zhaoping; Guo, Weijie; Wang, Qifeng; Chen, Zhiao; Huang, Shenglin; Zhao, Fangyu; Yao, Ming; Zhao, Yingjun; He, Xianghuo

doi:10.1053/j.gastro.2015.07.050

Cited by 76 publications

(65 citation statements)

References 58 publications

(17 reference statements)

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“…The reduction of nucleotide content upon SRC-2/RPIA knockdown most likely directly blocked DNA replication, leading to a decrease in EC cells entering S phase of the cell cycle. This would be in agreement with findings by other groups, who showed that RPIA maintains proliferation of pancreatic adenocarcinoma, hepatocellular carcinoma, and colorectal adenocarcinoma cells 42–44 . Importantly, reduction of DNA synthesis was also previously noted when RPIA levels were downregulated 42 .…”

Section: Discussionsupporting

confidence: 94%

Steroid Receptor Coactivator-2 Controls the Pentose Phosphate Pathway through RPIA in Human Endometrial Cancer Cells

Szwarc

Kommagani

Putluri

et al. 2018

Sci Rep

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Steroid receptor coactivator-2 (SRC-2) is a transcriptional coregulator that modulates the activity of many transcription factors. Levels of SRC-2 are elevated in endometrial biopsies from polycystic ovary syndrome patients, a population predisposed to endometrial cancer (EC). Increased expression of SRC-2 is also detected in neoplastic endometrium suggesting a causal link between elevated SRC-2 expression and the emergence of endometrial disorders that can lead to cancer. Here, we reveal that SRC-2 knockdown reduces EC cell proliferation and anchorage-independence. Additionally, SRC-2 is required to maintain cellular glycolytic capacity and oxidative phosphorylation, processes essential for EC cell proliferation. Importantly, SRC-2 is critical for the normal performance of the pentose phosphate pathway (PPP). Perturbation of the PPP due to loss of SRC-2 expression may result from the depletion of ribose-5-P isomerase (RPIA), a key enzyme of the PPP. As with SRC-2, RPIA knockdown reduces EC cell proliferation, which is accompanied by a decrease in glycolytic capacity and oxidative phosphorylation. Glucose metabolite tracking experiments confirmed that knockdown of SRC-2 and RPIA downregulates the metabolic rate of both glycolysis and the PPP, highlighting a novel regulatory cross-talk between glycolysis and the PPP modulated by SRC-2.

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Section: Discussionsupporting

confidence: 94%

Steroid Receptor Coactivator-2 Controls the Pentose Phosphate Pathway through RPIA in Human Endometrial Cancer Cells

Szwarc

Kommagani

Putluri

et al. 2018

Sci Rep

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“…For example, phosphoribosyl pyrophosphate synthetase 1 (PRPS1) was reported as a key enzyme involved in the pentose phosphate pathway, which is of particular importance for glucose metabolism in cancer cells (29). PRPS1 was upregulated significantly at the mRNA and protein levels in colorectal cancer (CRC) tissues, and the high expression of PRPS1 predicted a poor prognosis of CRC patients (29). These findings were similar to those we observed in this study of murine lung ADCs.…”

Section: Discussionsupporting

confidence: 85%

“…The overlapped gene set was named "Piog_Glu_LungADC," and many of the glucose metabolism genes in this gene set have been implicated in cancers. For example, phosphoribosyl pyrophosphate synthetase 1 (PRPS1) was reported as a key enzyme involved in the pentose phosphate pathway, which is of particular importance for glucose metabolism in cancer cells (29). PRPS1 was upregulated significantly at the mRNA and protein levels in colorectal cancer (CRC) tissues, and the high expression of PRPS1 predicted a poor prognosis of CRC patients (29).…”

Section: Discussionmentioning

confidence: 99%

Pioglitazone-mediated reversal of elevated glucose metabolism in the airway epithelium of mouse lung adenocarcinomas

Xiong¹,

Pan

Zhang

et al. 2017

JCI Insight

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“…miRNAs also serve as tumor promoter genes or tumor suppressor genes by negatively regulating their targets. These data suggest a possibility that miRNA is a novel focus for examining the current diagnosis and treatment of tumors.Previously, several studies revealed that miR-124 results in a decrease in the proliferation ability of CRC cells by targeting ribose-phosphate pyrophosphokinase 1 and ribose 5-phosphate isomerase mRNAs (12), that miR-146a directs the symmetric division of colorectal cancer stem cells (13) and that miR-101 serves as an endogenous proteasome inhibitor that suppresses tumor cell proliferation via targeting of the proteasome assembly factor proteasome maturation protein (14). Therefore, attention has been focused on the activities of miRNA in cancer development.…”

mentioning

confidence: 99%

MicroRNA-552 promotes tumor cell proliferation and migration by directly targeting DACH1 via the Wnt/β-catenin signaling pathway in colorectal cancer

et al. 2017

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Abstract. The purpose of the present study was to analyze the crucial role of microRNAs (miRNAs/miRs) involved in the proliferation and migration of colorectal cancer (CRC) and to investigate their underlying mechanisms. The present study discusses the expression and function of miR-552 in CRC. The expression level of miR-552 in CRC cells and tissues was observed, and it was suggested that the high expression of miR-552 accelerated the proliferation and migration of CRC cells in vitro. Notably, a result of the present study was that the cell fate determination factor Dachshund family transcription factor 1 (DACH1) was identified as a direct target of miR-552. Suppressing miR-552 expression in CRC cells increased endogenous DACH1 mRNA and protein levels, which was negatively correlated with miR-552. DACH1 performs an important role in the development of a number of neoplasms, and has the ability to regulate the Wnt/β-catenin signaling pathway as a novel predictive and diagnostic biomarker. Accordingly, it was concluded that miR-552 exerted a tumor-promoting role in CRC development by targeting DACH1, which may contribute to the increase in the rates of CRC proliferation and migration. miR-552 may serve as a potential diagnostic and prognostic biomarker for CRC. IntroductionColorectal cancer (CRC) has one of the highest cure rates of all types of malignant tumors (1), however remains ranked as the fourth leading cause of cancer-associated mortality in the world (2). In previous years, the morbidity and mortality rates of CRC have significantly increased due to an ageing population, and with changes in eating habits and lifestyles (3). The development of distant metastasis is a major cause of cancer-associated mortalities in CRC patients (4). Overwhelming evidence has demonstrated that aberrant expression of microRNA (miRNA/miR) contributes to CRC development by affecting the expression of the genes that regulate cancer progression (5).miRNAs, endogenous small non-coding regulatory RNAs measuring 18-25 nucleotides long (6), usually regulate gene expression in a number of tumor-associated signaling pathways at the post-transcriptional level, including the Wnt/β-catenin signaling pathway (7). As miRNAs tend to be localized to fragile chromosomal regions (8), they have the ability to adjust the levels of their corresponding mRNAs, and serve critical roles in the physiological and pathological processes of tumor development, which are a novel aspect of cancer studies. Previous evidence has demonstrated that miRNAs are involved in a number of biological processes, including proliferation, differentiation, migration, angiogenesis and protein splitting (9-11). miRNAs also serve as tumor promoter genes or tumor suppressor genes by negatively regulating their targets. These data suggest a possibility that miRNA is a novel focus for examining the current diagnosis and treatment of tumors.Previously, several studies revealed that miR-124 results in a decrease in the proliferation ability of CRC cells by targeting ribose-phosp...

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MicroRNA-124 Reduces the Pentose Phosphate Pathway and Proliferation by Targeting PRPS1 and RPIA mRNAs in Human Colorectal Cancer Cells

Cited by 76 publications

References 58 publications

Steroid Receptor Coactivator-2 Controls the Pentose Phosphate Pathway through RPIA in Human Endometrial Cancer Cells

Steroid Receptor Coactivator-2 Controls the Pentose Phosphate Pathway through RPIA in Human Endometrial Cancer Cells

Pioglitazone-mediated reversal of elevated glucose metabolism in the airway epithelium of mouse lung adenocarcinomas

MicroRNA-552 promotes tumor cell proliferation and migration by directly targeting DACH1 via the Wnt/β-catenin signaling pathway in colorectal cancer

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