MicroRNA‑1236‑3p inhibits human osteosarcoma growth

Abstract

Osteosarcoma (OS) is a common bone tumor with high mortality worldwide. The long-term survival rate of patients with metastatic or recurrent disease is <20%. The present study explored the biological role of microRNA (miRNA/miR)-1236-3p in OS. miRNA and mRNA expression levels were measured via reverse transcription-quantitative PCR. Fluorescence in situ hybridization was performed to determine miR-1236-3p expression levels in clinical specimens. Protein expression was measured via western blotting. Immunohistochemical analysis was used to detect Wnt target gene expression in tumor tissues. The interaction between the Wnt3a 3′untranslated region and miR-1236-3p was assessed via dual-luciferase reporter assays. Cell cycle, Transwell, Cell Counting Kit-8 and wound healing assays were conducted to evaluate the function of the miR-1236-3p/Wnt3a axis. Human OS (HOS) cells stably transfected with vector or miR-1236-3p sponge were injected subcutaneously into nude mice to assess the role of miR-1236-3p in vivo . miR-1236-3p expression was downregulated in OS tissues compared with chondroma tissues, and miR-1236-3p overexpression inhibited OS cell migration and proliferation compared with the negative control group. Furthermore, in vivo xenograft assays displayed enhanced tumour growth rates in the miR-1236-3p sponge group compared with the vector control group. In the present study, the results indicated that miR-1236-3p inhibited OS progression and Wnt3a was identified as a target of miR-1236-3p.

Anticancer effects of melatonin via regulating lncRNA JPX‐Wnt/β‐catenin signalling pathway in human osteosarcoma cells

“… 81 MiR‐1236‐3p inhibits the progression of OS via targeting Wnt3a 3’ UTR. 82 Similarly, in this study, the Wnt/β‐catenin pathway was also inhibited by melatonin. This inhibitory role of melatonin in the Wnt/β‐catenin pathway was restricted by lncRNA JPX overexpression.…”

“…Cinnamaldehyde promotes the cell apoptosis and inhibits the cell proliferation, migration and invasion in OS cells via inhibiting the Wnt/β-catenin pathway 81. MiR-1236-3p inhibits the progression of OS via targeting Wnt3a 3' UTR 82. Similarly, in this study, the Wnt/βcatenin pathway was also inhibited by melatonin.…”