Micropropagation ofUraria picta, a medicinal plant, through axillary bud culture and callus regeneration

Anand, Ajith; Rao, Chandrasekhara; Rangan, Latha; Josekutty, P. C.; Balakrishna, P.

doi:10.1007/bf02822778

Cited by 16 publications

(16 citation statements)

References 6 publications

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“…The natural and synthetic purine cytokinins are degraded to adenine and related nucleotides; therefore, the addition of adenine to the medium may retard the degradation of cytokinins by feed-back inhibition or by competing for the Adenine sulphate has been used for in vitro multiplication of Carica papaya (Saha et al 2004;Schmildt et al 2007), Musa sp. (Schmildt et al 2007) and Uraria picta (Anand et al 1998). Moreover, Nandagopal and Ranjitha Kumari (2006) used AS for shoot organogenesis induction in Cychorium intybus cv.…”

Section: Discussionmentioning

confidence: 99%

In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate

Gatica-Arias¹,

Valverde²,

Fonseca³

et al. 2010

Electro Journal of Biotech

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Section: Discussionmentioning

confidence: 99%

In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate

Gatica-Arias¹,

Valverde²,

Fonseca³

et al. 2010

Electro Journal of Biotech

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“…ex DC. (Anand et al, 1998) and for Albizia falcataria (L.) Fosberg (Widiyanto et al, 2008), two other Fabaceae. The rooting in this condition was approximately 40% less than in culture medium supplemented with 0.107 μM NAA, which can be attributed to the inhibitory effects of BAP on the rhizogenesis.…”

Section: An Efficient Establishment Of M Pudica In Vitro Culturesmentioning

confidence: 99%

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Bianchetti¹,

Resende²,

Pacheco³

et al. 2017

Afr. J. Biotechnol.

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This work aimed to develop a protocol for the in vitro establishment, multiplication, rooting and ex vitro acclimatization of Mimosa pudica L., a species used in folk medicine and with pharmacological activity. Aseptic cultures were established from seeds inoculated in MS medium, without growth regulators, followed by an in vitro stabilization phase in culture medium supplemented with 2.22 μM BAP. The cultures were transferred to MS medium supplemented with different cytokinins, combined or not with an auxin, aiming its large-scale propagation. The culture medium supplemented with 5 µM BAP plus 0.5 µM NAA provided the highest multiplication rate and top quality plantlets. The combination of 0.6 µM TDZ plus 0.05 µM NAA resulted in higher multiplication rates than in response to combination of BAP plus NAA, although the subsequent maintenance of the cultures in a medium without growth regulators has resulted in low regenerative response. In vitro rooting of micro-cuttings was high even in the absence of auxins. Over 90% of plantlets transferred to the greenhouse survived after the acclimatization phase. Acclimatized plants presented normal vegetative and reproductive development. The procedures established in the present study allow a massive production of M. pudica plants for further pharmacological studies.

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“…picta (tribe=Hedysarea) is an erect, basal-woody, annual herb of height ranging from 0.5-2 m with juvenile simple leaves to mature odd-pinnate compound leaves arranged in an increasing odd number order (1,3,5,7,9,11) from one node to the next above it. Lateral veins of leaves looped with margin.…”

Section: Plant Distribution and Descriptionmentioning

confidence: 99%

Morphological and anatomical investigations into the mechanism of leaf pair unrolling in <i>Uraria picta</i> (jacq.) desv. ex dc. (papilionaceae), a medicinal plant in Nigeria

Oyesiku

Okusanya²,

Olowokudejo³

2013

Afr. J. Trad. Compl. Alt. Med.

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Uraria picta leaf-pair unrolling inside out is a remarkable feat. A leaf-pair was investigated to understand the mechanism of spontaneous reverse inside out act of the plant. The upper (adaxial) and lower (abaxial) surfaces of the leaf-pair were examined using scanning and light microscopy. The scan showed diversity of hairs varying in shape from straight, pointed, curve, and club to hook. There were deposits of wax on both sides of the leaf-pair. The light microscope showed hairs are restricted to the midrib on the adaxial surface of the leaflets. Hooked hairs dominated the entire abaxial surface of the leaflets. The transverse section of the midrib section showed abundance of structural and mechanical tissues, collenchyma and sclerenchyma tissues. Both morphological and anatomical attributes were used to explain the mechanism and how the plant got its Yoruba vernacular name 'Alupayida' as well as its purported use in changing the sex of the unborn child and in breaking up love affairs.

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Micropropagation ofUraria picta, a medicinal plant, through axillary bud culture and callus regeneration

Cited by 16 publications

References 6 publications

In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate

In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate

An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.

Morphological and anatomical investigations into the mechanism of leaf pair unrolling in <i>Uraria picta</i> (jacq.) desv. ex dc. (papilionaceae), a medicinal plant in Nigeria

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