In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate

Effect of Gamma Irradiation and Selection with Fungus Filtrate (Rhizoctonia solani Kuhn) on the in Vitro Culture of Common Bean (Phaseolus vulgaris)

“…Seeds of P. vulgaris var. Bribri were disinfected following the method of Gatica et al [18]. Embryonic axes (EAs) were excised using a scalpel and tweezers under a microscope stereo.…”

Ultrastructural Analysis of the Ontogenetic Development of Shoot Induced from Embryonic Axes of Costa Rican Bean Varieties (<i>Phaseolus vulgaris</i> L.) under <i>in Vitro</i> Conditions by Scanning Electronic Microscopy

“…Zygotic embryos from the Costa Rican bean varieties Guaymí, Bribrí, Huetar, and Brunca were used because these varieties are the most consumed in our country. These zygotic embryos were used, according with experimental protocols developed in Reference [5].…”

“…Species of Phaseolus are considered recalcitrant to in vitro culture and transformation [3,4]. Successful procedures for the in vitro culture and plant regeneration of bean varieties have been only developed in few laboratories around the world [1,5]. Reference [5] studied the effect of BAP and AS on the number of shoots induced from embryogenic axes of Costa Rican bean varieties (Bribrí, Brunca, Guaymí, Huetar and Telire) embryogenic axes were cultivated on induction medium supple-mented using 5 mg•l -1 BAP combined with 20 mg•l -1 AS or 5 mg•l -1 BAP with 40 mg•l -1 AS.…”

“…Reference [5,6] the morphological characteristics of apical buds and the effects of BAP on multiple shoot induction is important for assessing important traits in the apical meristem. This information is very important because the response of common bean varieties grown in Costa Rica to tissue culture techniques is unknown.…”

Evaluation of conditions affecting Agrobacterium-mediated genetic transformation of soybean (Glycine max L.) and common bean (Phaseolus vulgaris L.)

“…Regeneration can be achieved by using various methods, either direct or indirect, and organogenesis or embryogenesis, often determined based on the target tissue for regeneration or transformation (explant). Arellano et al, 2009;Arias et al, 2010;Kwapata et al, 2010;Quintero-Jiménez et al, 2010;Collado et al, 2013) with variation reported in shoot formation (Arias et al, 2010;Chandel and Pandey, 2014) and regeneration response between cultivars (McClean and Grafton, 1989;Arellano et al, 2009;Kwapata et al, 2010;Quintero-Jiménez et al, 2010).…”