Micropropagation of Vaccinium corymbosum L.: An Alternative Procedure for the Production of Secondary Metabolites

Abstract

In vitro culture has become a dependable approach for the mass production of plant material as the market for innovative plant-derived medicinal approaches has grown significantly. Furthermore, because it permits manipulation of biosynthetic routes to boost the production and accumulation of certain compounds, this technology has enormous potential for the manufacture of natural bioactive chemicals. As a result, the goal of this study was to develop an efficient micropropagation system for biomass production and to investigate the accumulation of bioactive compounds from Vaccinium corymbosum L., Duke and Hortblue Petite cultivars. Two in vitro plant tissue culture systems were used for shoots production: a solid medium (5 g/L Plant agar) and liquid medium (Plantform bioreactor). The culture medium used was Woddy Plant Medium (WPM) supplemented with two growth regulators: 0.5 mg/L and 1 mg/L zeatina (Z) and 5 mg/L N6-(2-Isopentenyl) adenine (2iP). The content of phenolic compounds, carotenoids, and chlorophylls of the in vitro shoot extracts were examined via the HPLC-DAD-MS/MS technique. The results showed that cv. Hortblue Petite produced a higher amount of biomass compared with cv. Duke, on all variants of culture media in both systems (solid and liquid), while the shoots extract of the Duke variety in the liquid culture system (under all concentrations of growth regulators) had the highest content of total phenolic compounds (16,665.61 ± 424.93 μg/g). In the case of the lipophilic compounds analysed (chlorophylls and carotenoids), the solid medium reported the highest values, whereas media supplemented with 0.5 mg/L Z was proved to have the richest total content for both cultivars.

Autopolyploidization and in vitro regeneration of three blueberry cultivars from leaves and microstems.

“…Notably, 'Legacy' exhibited a more robust regenerative response in the presence of ZT than the other cultivars (Fig. 1), supporting the regeneration disparities between genotypes documented in the literature (Clapa et al 2022). This indicates that the addition of growth regulators notably interacts with explant´s endogenous hormones (Gahan and George 2008), generating different responses per cultivar depending on their proper inner hormone levels or sensitivity.…”

“…1f), as expected due to its known function in promoting axillary bud growth (Tamas 1995). With an average of three or more shoots per microstem explant, it con rmed the positive response of microstem regeneration to ZT (Clapa et al 2022).…”

“…PM and regeneration medium (RM), 0.5 and 1 mg/L of ZT respectively (Table 1), were assessed for their e cacy in inducing multiple shoot regeneration in vitro from blueberry leaves and 3-node stem segments (from now on called microstems) (Clapa et al 2022). Explants in basal medium (BM) were used as control treatment (Table 1).…”

Polysaccharide-Based Edible Gels as Functional Ingredients: Characterization, Applicability, and Human Health Benefits

“…3D food printing is an excellent tool for personalised nutrition about particular dietary necessities (e.g., obesity). It can be an ideal instrument to prevent different non-communicable diseases (e.g., gut microbiota, intestinal microecology) through improved functional food products containing bioactive compounds (e.g., antioxidants, phytonutrients, proteins, and probiotics) [ 83 , 94 , 95 , 96 ]. The compatibility between bigels, serving as vehicles for both hydrophilic and lipophilic bioactive molecules, and personalized 3D food printing might have impactful results regarding the prevention or the treatment of individual needs in humans.…”

Vaccinium Species (Ericaceae): Phytochemistry and Biological Properties of Medicinal Plants

“…Due to its various health-related benefits, its consumption is in constant growth, being efficient in relation to diabetes, obesity, cardiovascular and neurodegenerative diseases, atherosclerosis, rheumatoid arthritis, and cancer [ 17 , 18 ]. Based on several studies, the leading health benefits are accredited to their antioxidant, antimicrobial, and detoxifying effects on the organism [ 19 , 20 , 21 ]. Additionally, it is considered that these plants, particularly those with high polyphenol quantity such as anthocyanins, are able to hinder and initiate apoptosis within cancer cells [ 22 ].…”