Micropropagation of threatened black alder

José, Mario; Janeiro, Laura V.; Corredoira, Elena

doi:10.14214/sf.892

Cited by 12 publications

(7 citation statements)

References 35 publications

(38 reference statements)

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“…The present findings showed that holm oak tree genotypes derived from 30 to 100 yr-old in situ selected ortets could be established in vitro, although differences were observed between the clones, both at the culture initiation stage and during the subsequent micropropagation by axillary budding. Differences between species and also between different genotypes of the same species have been reported in woody plants such as American oak species (Vieitez et al 2009), strawberry tree (Gomes et al 2010) or black alder (San José et al 2013).…”

Section: Discussionmentioning

confidence: 97%

Micropropagation of mature Quercus ilex L. trees by axillary budding

Martínez

Corredoira

Viéitez

et al. 2017

Plant Cell Tiss Organ Cult

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This paper reports the successful micropropagation of mature Quercus ilex trees known as reluctant to in vitro propagation. Crown branch segments collected from 30 and100 year-old trees were forced in order to promote the production of sprouting shoots that were used as a source of explants for initiating the cultures. Sterilization was critical and required low-level disinfestation protocols. Six out of the eight mature genotypes attempted were successfully inoculated and then maintained in culture with varying responses. Shoot proliferation of holm oak was influenced by BA concentration, with improved multiplication and shoot appearance when the BA concentration was sequentially reduced over the culture period. Micropropagation by axillary budding was achieved by culturing shoots on a sequence of cytokinin-enriched Lloyd and McCown (WPM) media alternating 2 week-long subcultures on 0.44 µM benzyadenine (BA) first, followed by 0.22 µM BA, then 0.044µM BA plus 0.46µM zeatin. Sucrose concentration and agar brand affected shoot proliferation, and the best results were obtained on WPM medium supplemented with 8 g L-1 Sigma agar (A-1296; Sigma-Aldrich) and 30 g L-1 sucrose. Addition of 20 µM silver thiosulphate had a significant positive effect on the appearance and development of shoots with a higher number of shoots being healthy and showing reduced shoot tip necrosis and early senescence of leaves. The 18.8% of the microshoots obtained for one clone could be rooted within 15 days on a half-strength Murashige and Skoog medium containing 14.8µM or 24.6µM indole-3-butyric acid and 0.54 µM -naphthalene acetic acid.

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Section: Discussionmentioning

confidence: 97%

Micropropagation of mature Quercus ilex L. trees by axillary budding

Martínez

Corredoira

Viéitez

et al. 2017

Plant Cell Tiss Organ Cult

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“…Stock shoot cultures (Clones G1, R1 and R4) were established in vitro from mature A. glutinosa trees in 2009, as previously described (San José et al 2013). Clonal shoot multiplication was maintained by subculturing shoots in 500 ml glass jars containing 70 ml of Woody Plant Medium (WPM, Lloyd and McCown 1980), supplemented with 0.1 mg l -1 6-benzylaminopurine, 0.5 mg l -1 indole-3-acetic acid, 20 g l -1 glucose and 7 g l -1 Difco agar (proliferation medium).…”

Section: Plant Materials and Culture Conditionsmentioning

confidence: 99%

“…by in vitro methods was made in this study. Shoot explants from different mature trees were successfully cultured in vitro (San José et al 2013), and the micropropagated shoots were maintained without loss of the multiplication capacity for over five years. However, shoot cultures required regular 3-week subculture at 25ºC and 16 h photoperiod, and therefore the development of reliable methods of mediumterm storage is crucial.…”

Section: Introductionmentioning

confidence: 99%

Simple strategy for the in vitro conservation of Alnus glutinosa (L.) Gaertn. germplasm

San-José

Janeiro

Corredoira

2014

Trees

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The aim of this study was to develop a simple method for the medium-term storage of Alnus glutinosa (L.) Gaertn. explants obtained from trees aged 20-30 years. Several parameters were evaluated, including type of explant (shoot apex or nodal segments), pre-storage treatment (0 or 10 days after the last subculture) and duration of cold storage (3-24 months) at 2-4ºC. Explants were maintained at this temperature under dim lighting on Woody Plant Medium supplemented with 0.1 mg l-1 6-benzyladenine and 0.5 mg l-1 indole-3-acetic acid. Under these conditions, a high percentage (75-87%) of cultures remained viable after 18 months in cold cabinets. The stored material was successfully recovered and multiplied normally in the same medium, showing good growth and developing into normal shoots that were morphologically similar to those of non-stored controls. At the histological level, the main change observed was the accumulation of starch granules in cells of the shoot apex, as well as in cells located close to the vascular bundles, after 3 months of cold storage. As the duration of cold storage increased, the number and size of the starch granules decreased but cell plasmolysis and the content of lipid droplets increased. Cold damage was generalized after 24 months at 4ºC. This study provides new insights into the changes occurring in A. glutinosa during cold storage.

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“…A recent review has described forest biotechnology techniques as an emerging opportunity for tree improvement and conservation in Quercus species (Vieitez & al., 2012). Micropropagation techniques represent an alternative approach for the rapid propagation and improvement of forest stock, especially when conventional propagation is difficult to achieve (Renau-Morata & al., 2005;Gomes & Canhoto, 2009;San José & al., 2013). In vitro culture techniques also allow conservation of species with low seed viability, establishment of cultures for clonal mass propagation, and production of highly heterozygous crops or cultures that must be propagated vegetatively to preserve their genetic integrity (Corredoira & al., 2011).…”

Section: Introductionmentioning

confidence: 99%

Conservation of holm oak (Quercus ilex) by in vitro culture

et al. 2018

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Abstract. In vitro culture techniques are used to propagate tree species, as well as to conserve the species in the short and long term. In the present study, in vitro propagation and conservation of holm oak (Quercus ilex L.) were successfully achieved using juvenile material. Mature acorns were germinated under controlled conditions of moisture and temperature, and 3-month-old seedlings were used as source of explants for culture initiation. Micropropagation via axillary bud proliferation was achieved by culturing shoots in a vertical position on Woody Plant Medium containing different cytokinins and/or concentrations, which were changed every 2 weeks over a 6-week multiplication cycle, as follows: 0.1 mg L -1 benzyladenine (BA) for the first 2 weeks, 0.05 mg L -1 BA for the next 2 weeks, and 0.01 mg L -1 BA plus 0.1 mg L -1 zeatin for the last 2 weeks. Acceptable rooting rates were obtained by culturing microcuttings in Murashige & Skoog medium with half-strength macronutrients supplemented with 3 or 5 mg L -1 indole-3-butyric acid (IBA) in combination with 0.1 mg L -1 naphthalene acetic acid (NAA) for 15 days and subsequent transfer to auxin-free medium for 4 weeks. Keywords: Axillary shoot proliferation; In vitro conservation; Micropropagation; Phytophthora cinnamomi; Seedlings. Conservación de Quercus ilex mediante cultivo in vitroResumen. Las técnicas de cultivo in vitro permiten la propagación de especies leñosas, así como su conservación a corto y largo plazo. En este trabajo se logró, con éxito, la propagación y la conservación in vitro de encina (Quercus ilex L.) partiendo de material juvenil. Los explantos utilizados para el establecimiento in vitro se obtuvieron a partir de plántulas de 3 meses procedentes de bellotas germinadas bajo condiciones controladas de humedad y temperatura. Los brotes establecidos in vitro se micropropagaron vía proliferación de yemas axilares cultivándolos en posición vertical en medio Woody Plant Medium durante 6 semanas con transferencias a medio fresco cada 2 semanas donde se modifica el tipo de citoquinina y su concentración, como sigue: 0,1 mg L -1 benciladenina (BA) durante las 2 primeras semanas, transferencia a 0,05 mg L -1BA las 2 semanas siguientes, y por último se transfieren a 0,01 mg L -1 BA más 0,1 mg L -1 zeatina durante 2 semanas más, hasta completar las seis semanas. Las tasas de enraizamiento fueron aceptables cuando los brotes se cultivaron en medio Murashige & Skoog con los macronutrientes reducidos a la mitad adicionado con 3 ó 5 mg L -1 de ácido indol-3-butírico (AIB) en combinación con 0,1 mg L -1 de ácido naftalenacético (ANA) durante 15 días y posterior transferencia al mismo medio sin auxinas durante 4 semanas. Palabras clave: Proliferación vía yemas axilares; conservación in vitro; Micropropagación; Phytophthora cinnamomi; plántulas.

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Micropropagation of threatened black alder

Cited by 12 publications

References 35 publications

Micropropagation of mature Quercus ilex L. trees by axillary budding

Micropropagation of mature Quercus ilex L. trees by axillary budding

Simple strategy for the in vitro conservation of Alnus glutinosa (L.) Gaertn. germplasm

Conservation of holm oak (Quercus ilex) by in vitro culture

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