Micropropagation of mature Quercus ilex L. trees by axillary budding

Martínez, M. T.; Corredoira, Elena; Viéitez, A. M.; Cernadas, Ma José; Montenegro, Raquel; Ballester, A.; Vieitez, F. J.; San-José, M. C.

doi:10.1007/s11240-017-1300-x

Cited by 38 publications

(31 citation statements)

References 58 publications

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“…In Q. serrata, WPM with 2 mg·L −1 BA produced the highest number of shoots [31]. While highest shoot proliferation rate of Q. ilex was achieved by alternating 2 week long subcultures on much lower concentration of cytokinin, which was 0.1 mg·L −1 BA first, followed by 0.05 mg·L −1 BA, then 0.01 mg·L −1 BA plus 0.1 mg·L −1 zeatin [32]. The effect of genotype on the optimal protocol was shown to be one of the most serious limitations concerning the commercial micropropagation of oaks [21].…”

Section: Discussionmentioning

confidence: 96%

“…The effect of genotype on the optimal protocol was shown to be one of the most serious limitations concerning the commercial micropropagation of oaks [21]. Other components such as sucrose concentration and agar brand also affected shoot proliferation in Q. ilex [32]. Other basal media, such as plant growth regulators can also be trialed, however only BA and IBA were chosen in this study as these two plant growth regulators were the most commonly used.…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Propagation of Oriental White Oak Quercus aliena Blume

Li¹,

Gu²,

Deng

2019

Forests

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Quercus aliena Blume, also known as the oriental white oak, is a widespread species in temperate forests of East Asia with significant ecological and economical importance. Establishing an efficient vegetative propagation system is important for its germplasm conservation and breeding program. Protocols of micropropagation from shoot tips and nodal segments were investigated in order to produce uniform high-quality seedlings. Nodal segments from 18 month old seedlings were used as explants to initiate the aseptic culture. The highest bud proliferation was achieved by subculturing the explants on 1/2 strength woody plant medium (WPM) with 2.0 mg·L −1 BA. WPM with 0.5 mg·L −1 BA and 0.05 mg·L −1 IBA was the best medium for subculture to obtain the vigorous regenerated shoots in this experiment. Nodal segments without shoot tips had a higher adventitious bud proliferation rate than those with shoot tips. The highest rate (41.5%) of rooting in vitro was induced by using WPM with 1.0 mg·L −1 IBA and 5 g·L −1 activated charcoal. Ex vitro rooting by dipping the proliferated shoots with 500 mg·L −1 IBA solution, then transplanting directly to potting mix with 50% peat and 50% horticultural perlite fostered the highest rooting percentage and survival rate of the plantlets.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

In Vitro Propagation of Oriental White Oak Quercus aliena Blume

Li¹,

Gu²,

Deng

2019

Forests

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“…Similarly, the proliferation of shoots established from mature material of holm oak is not influenced by the explant type (Martínez & al., 2017a).…”

Section: Discussionmentioning

confidence: 99%

“…On the basis of our previous experience with adult holm oak material (Martínez & al., 2017a), the multiplication medium used consisted of WPM supplemented with 30 g L -1…”

Section: Shoot Multiplication Stagementioning

confidence: 99%

Conservation of holm oak (Quercus ilex) by in vitro culture

et al. 2018

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Abstract. In vitro culture techniques are used to propagate tree species, as well as to conserve the species in the short and long term. In the present study, in vitro propagation and conservation of holm oak (Quercus ilex L.) were successfully achieved using juvenile material. Mature acorns were germinated under controlled conditions of moisture and temperature, and 3-month-old seedlings were used as source of explants for culture initiation. Micropropagation via axillary bud proliferation was achieved by culturing shoots in a vertical position on Woody Plant Medium containing different cytokinins and/or concentrations, which were changed every 2 weeks over a 6-week multiplication cycle, as follows: 0.1 mg L -1 benzyladenine (BA) for the first 2 weeks, 0.05 mg L -1 BA for the next 2 weeks, and 0.01 mg L -1 BA plus 0.1 mg L -1 zeatin for the last 2 weeks. Acceptable rooting rates were obtained by culturing microcuttings in Murashige & Skoog medium with half-strength macronutrients supplemented with 3 or 5 mg L -1 indole-3-butyric acid (IBA) in combination with 0.1 mg L -1 naphthalene acetic acid (NAA) for 15 days and subsequent transfer to auxin-free medium for 4 weeks. Keywords: Axillary shoot proliferation; In vitro conservation; Micropropagation; Phytophthora cinnamomi; Seedlings. Conservación de Quercus ilex mediante cultivo in vitroResumen. Las técnicas de cultivo in vitro permiten la propagación de especies leñosas, así como su conservación a corto y largo plazo. En este trabajo se logró, con éxito, la propagación y la conservación in vitro de encina (Quercus ilex L.) partiendo de material juvenil. Los explantos utilizados para el establecimiento in vitro se obtuvieron a partir de plántulas de 3 meses procedentes de bellotas germinadas bajo condiciones controladas de humedad y temperatura. Los brotes establecidos in vitro se micropropagaron vía proliferación de yemas axilares cultivándolos en posición vertical en medio Woody Plant Medium durante 6 semanas con transferencias a medio fresco cada 2 semanas donde se modifica el tipo de citoquinina y su concentración, como sigue: 0,1 mg L -1 benciladenina (BA) durante las 2 primeras semanas, transferencia a 0,05 mg L -1BA las 2 semanas siguientes, y por último se transfieren a 0,01 mg L -1 BA más 0,1 mg L -1 zeatina durante 2 semanas más, hasta completar las seis semanas. Las tasas de enraizamiento fueron aceptables cuando los brotes se cultivaron en medio Murashige & Skoog con los macronutrientes reducidos a la mitad adicionado con 3 ó 5 mg L -1 de ácido indol-3-butírico (AIB) en combinación con 0,1 mg L -1 de ácido naftalenacético (ANA) durante 15 días y posterior transferencia al mismo medio sin auxinas durante 4 semanas. Palabras clave: Proliferación vía yemas axilares; conservación in vitro; Micropropagación; Phytophthora cinnamomi; plántulas.

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“…Vieitez et al ( 2009 ) reduced the incidence of STN in northern red oak ( Quercus rubra L.) cultures by supplementing medium with 3 mg/l (17.6 µM) AgNO 3 . Martínez et al ( 2017 ) found that AgNO 3 at 20 µM reduced the incidence of STN in evergreen oak ( Quercus ilex L.) cultures. Park et al ( 2016 ) found that the production of ethylene in rose ( Rosa hybrida cv.…”

Section: Plant Growth Regulators Affect Stnmentioning

confidence: 99%

Shoot tip necrosis of in vitro plant cultures: a reappraisal of possible causes and solutions

Silva

Nezami-Alanagh

Barreal

et al. 2020

Planta

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Main conclusion Shoot tip necrosis is a physiological condition that negatively impacts the growth and development of in vitro plant shoot cultures across a wide range of species. Abstract Shoot tip necrosis is a physiological condition and disorder that can arise in plantlets or shoots in vitro that results in death of the shoot tip. This condition, which can spread basipetally and affect the emergence of axillary shoots from buds lower down the stem, is due to the cessation of apical dominance. STN can occur at both shoot multiplication and rooting stages. One of the most common factors that cause STN is nutrient deficiency or imbalance. Moreover, the presence or absence of plant growth regulators (auxins or cytokinins) at specific developmental stages may impact STN. The cytokinin to auxin ratio within an in vitro plant can be modified by varying the concentration of cytokinins used in the culture medium. The supply of nutrients to in vitro shoots or plantlets might also affect their hormonal balance, thus modifying the occurrence of STN. High relative humidity within culture vessels and hyperhydricity are associated with STN. An adequate supply of calcium as the divalent cation (Ca2+) can hinder STN by inhibiting the accumulation of phenolic compounds and thus programmed cell death. Moreover, the level of Ca2+ affects auxin transport and ethylene production, and higher ethylene production, which can occur as a result of high relative humidity in or poor ventilation of the in vitro culture vessel, induces STN. High relative humidity can decrease the mobility of Ca2+ within a plant, resulting in Ca2+ deficiency and STN. STN of in vitro shoots or plantlets can be halted or reversed by altering the basal medium, mainly the concentration of Ca2+, adjusting the levels of auxins or cytokinins, or modifying culture conditions. This review examines the literature related to STN, seeks to discover the associated factors and relations between them, proposes practical solutions, and attempts to better understand the mechanism(s) underlying this condition in vitro.

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Micropropagation of mature Quercus ilex L. trees by axillary budding

Cited by 38 publications

References 58 publications

In Vitro Propagation of Oriental White Oak Quercus aliena Blume

In Vitro Propagation of Oriental White Oak Quercus aliena Blume

Conservation of holm oak (Quercus ilex) by in vitro culture

Shoot tip necrosis of in vitro plant cultures: a reappraisal of possible causes and solutions

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