Micropropagation of the Halophyte Sarcocornia fruticosa (L.) A. J. Scott

Halophytes are species able to grow and complete their life cycle under NaCl concentrations above 0.2 M. In a context of growing population and uncertain agricultural food sustainability, there is an interest on halophytes as potential source of food and fodder. However, scarce research has addressed in vitro propagation of halophytes as a tool for study, conservation, and propagation of elite germplasm. In this study, an efficient method for micropropagation of the succulent and salt accumulator halophyte Arthrocaulon macrostachyum has been established for the first time, using shoot tips from in vitro-germinated plant material. During shoot multiplication stage, superior genotypes were selected from explants grown in high strength and NaCl content medium and subsequently rooted and acclimatized to ex vitro conditions. A comprehensive characterization including determination of oxidative stress, photosynthesis performance, and mineral nutrient contents was done. This research gains insight into the physiological and biochemical characterization of halophytes during micropropagation and provides a solid platform for the production of elite A. macrostachyum germplasm for ulterior uses.

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Effect of MgCl2 and double concentration of Murashige and Skoog medium on in vitro plantlet and root cultures generation in halophytic grasswort Salicornia brachiata

Singh

Jani

Kumari

et al. 2014

Plant Cell Tiss Organ Cult

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An improved micropropagation protocol has been developed for halophytic grasswort Salicornia brachiata by using double concentration of Murashige and Skoog (Physiol Plant 15:473-497, 1962) medium (DMS) supplemented with plant growth regulators. Best shoot bud induction (90 %) from nodal explants was observed on DMS supplemented with 3.0 mg L -1 6-benzyl aminopurine (BAP) and 0.5 mg L -1 Zeatin with[3 shoot buds. Shoot proliferation and elongation was achieved on DMS supplemented with 1.0 mg L -1 thidiazuron (TDZ) and 1.0 mg L -1 a-naphthalene acetic acid (NAA) with multiple shoot buds after 30 days of culture. Best callus response (78 %) was observed when stem explants were cultured on DMS supplemented with 2.0 mg L -1 2, 4-dichlorophenoxyacetic acid and 0.01 mg L -1 BAP. Regeneration from the callus was achieved when callus was cultured on DMS supplemented with 0.5 mg L -1 TDZ. These shoot buds were elongated on shoot proliferation and elongation medium. Elongated shoots (5 cm) could be rooted on DMS supplemented with 0.5-1.5 mg L -1 NAA, 0.5-1.5 mg L -1 indole-3-acetic acid and 0.5-1.5 mg L -1 indole-3-butyric acid. DMS medium supplemented with 0.5 mg L -1 NAA found to be best for rooting and the addition of 20 g L -1 magnesium chloride (MgCl 2 ) to the medium resulted in highest percentage of rooting and not with the addition of sodium chloride (NaCl). Rooted plants could be established in soil with 55 % survival. In another set of study on root culture generation, many roots initiated from explants cultured on DMS supplemented with 2.0 mg L -1 NAA. Addition of 10 g L -1 MgCl 2 was highly beneficial in stimulating root initiation and proliferation as compared to NaCl.

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Micropropagation of the Halophyte Sarcocornia fruticosa (L.) A. J. Scott

Cited by 4 publications

References 16 publications

Potentialities of Halophytes in the Egyptian Deserts as Economic Plants

Potentialities of Halophytes in the Egyptian Deserts as Economic Plants

Efficient In Vitro Platform for Multiplication, Acclimatization, and Deliver of High-NaCl-Tolerant Clones of the Halophyte Arthrocaulon macrostachyum

Effect of MgCl2 and double concentration of Murashige and Skoog medium on in vitro plantlet and root cultures generation in halophytic grasswort Salicornia brachiata

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