Effect of MgCl2 and double concentration of Murashige and Skoog medium on in vitro plantlet and root cultures generation in halophytic grasswort Salicornia brachiata

Singh, Aneesha; Jani, Kruti; Kumari, P.; Agarwal, Pradeep K.

doi:10.1007/s11240-014-0622-1

Cited by 11 publications

(8 citation statements)

References 30 publications

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“…Addition of NaCl to medium was extremely critical for the propagation of another halophilous plant, Salicornia brachiata, under in vitro conditions by Joshi et al [ 26 ]. In contrast to the study of Singh et al [ 33 ] and Joshi et al [ 34 ], in the present study the addition of NaCl to medium at concentrations of 25 and 50 mM neither stimulated the development of Honckenya nor had any negative impact on its growth, which may suggest the tolerance of the plant to soil salinity. However, increasing the soil salinity to 75 mM resulted in the development of the shortest shoots and shorter and less numerous roots.…”

Section: Discussioncontrasting

confidence: 99%

Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh

Kulpa

Wróbel

Bednarek

2020

Plants

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The sea sandwort—Honckenya peploides (L.) Ehrh. is—a rare halophilous plant growing on dunes and is an endangered species on the Polish coast. It contributes to the stabilization of volatile sandy substrate, facilitating the colonization of other species. The present study determined the reaction of two types of explant: apical shoot fragments and fragments from a lower portion of the shoot. Apical shoot fragments were used to propagate and root sea sandwort plants due to the positive impact on the development of shoots and roots. Regardless of the plant growth regulators applied in the medium, the lateral meristems on the explants from the lower parts of the shoot stopped growing, and then yellowed and died out. Apical fragments of shoots developed higher and more numerous shoots and longer and more numerous roots than explants, which were fragments collected from lower parts of shoots. The findings indicated that propagation should be conducted on Murashige and Skoog medium with the addition of 1 mg∙dm−3 kinetin, whereas shoots with their apical fragments should be rooted with the addition of 1.5 mg∙dm−3 1-naphthaleneacetic acid. The results also showed that the addition of NaCl at concentrations of 25 and 50 mM did not restrict their growth, thereby indicating the tolerance of the plant to soil salinity. However, an increase in the concentration of NaCl in the medium to 75 mM restricted the development of plants, and the shoots were lower and roots were shorter and less numerous.

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Section: Discussioncontrasting

confidence: 99%

Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh

Kulpa

Wróbel

Bednarek

2020

Plants

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“…Due to strong aggregation tendency of AuNPs in plant growth media (MS medium), commonly used for plant growth in vitro [ 23 ], we had to optimize mutual ratio of these two components to avoid NPs aggregation while preserving acceptable conditions for plant growth. Different dilutions of MS were tested.…”

Section: Resultsmentioning

confidence: 99%

Round-shape gold nanoparticles: effect of particle size and concentration on Arabidopsis thaliana root growth

et al. 2018

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Nowadays, due to a wide range of applications of nanoparticles (NPs) in many industrial areas, accumulations of those entities in environment pose a great risk. Owing to their inertness, noble metal NPs may remain in contaminated soils nearly unchanged for long time. Within this context, size-, shape-, and concentration-dependent uptake of particles by plants belongs to unexplored area. In this work, we present water solutions of biologically friendly synthesized spherical AuNPs with pretty narrow size distribution in size range from 10 to 18 nm. Their thorough characterization by atomic absorption spectroscopy, mass spectroscopy-equipped inductively coupled plasma, dynamic light scattering (DLS), and TEM methods was followed by the study of their effect on the growth of Arabidopsis thaliana (primary and lateral roots), in particle size- and concentration-dependent manner. Due to strictly round-shape form of AuNPs and absence of particle agglomeration, DLS-derived size and size distribution were in good concordance with those obtained from TEM. The length and number of A. thaliana lateral roots were significantly affected by all types of AuNPs. Smallest AuNPs at highest concentration inhibited length of primary roots and, in contrast, enhanced hair root growth.

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“…Plant development is inhibited and deformed as a result of nitrogen deficiency. The most effective MS medium was found to be MS medium with a standard amount of nitrogen source in micropropagation of Phyllanthus amarus [61].…”

Section: Discussionmentioning

confidence: 99%

The Efficient and Easy Micropropagation Protocol of Phyllanthus niruri

Suraya

Hakiman

2021

Plants

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Phyllanthus niruri (P. niruri) or Dukung Anak is a herbal plant in the Phyllanthaceae family that has been used traditionally to treat various ailments such as diabetes, jaundice, flu and cough. P. niruri contains numerous medicinal benefits such as anti-tumor and anti-carcinogenic properties and a remedy for hepatitis B viral infection. Due to its beneficial properties, P. niruri is overharvested and wild plants become scarce. This study was conducted to develop an appropriate in vitro culture protocol for the mass production of P. niruri. An aseptic culture of P. niruri was established followed by multiplication of explants using different types of basal medium and its strength and plant growth regulators manipulation. This study also established the induction of in vitro rooting utilizing various types and concentrations of auxin. Treatment of Clorox® with 30% concentration showed the lowest percentage (%) of contamination, 4.44% in P. niruri culture. Nodal segments of P. niruri were successfully induced in full-strength of Murashige and Skoog (MS) basal media with 2.33 number of shoots, 3.11 cm length of shoot and 27.91 number of leaves. In addition, explants in full-strength MS media without any additional cytokinin were recorded as the optimum results for all parameters including the number of shoots (5.0 shoots), the length of shoots (3.68 cm) and the number of leaves (27.33 leaves). Treatment of 2.5 µM indole-3-butyric acid (IBA) showed the highest number of roots (17.92 roots) and root length (1.29 cm). Rooted explants were transferred for acclimatization, and the plantlet showed over 80% of survival rate. In conclusion, plantlets of P. niruri were successfully induced and multiplied via in vitro culture, which could be a step closer to its commercialization.

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Effect of MgCl2 and double concentration of Murashige and Skoog medium on in vitro plantlet and root cultures generation in halophytic grasswort Salicornia brachiata

Cited by 11 publications

References 30 publications

Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh

Type of Explant Affects In Vitro Development and Multiplication Success of the Rare Halophyte Plant Honckenya Peploides L. Ehrh

Round-shape gold nanoparticles: effect of particle size and concentration on Arabidopsis thaliana root growth

The Efficient and Easy Micropropagation Protocol of Phyllanthus niruri

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