Micropropagation of Senecio Macrophyllus M. Bieb

In vitro slow growth storage of Senecio macrophyllus shoots

“…Multiple rates recorded after regrowth following the 3 months of storage in magenta boxes on medium with 4.4 BA were lower compared to results obtained during multiplication of non-stored shoots on a medium of the same composition of growth regulators, but similar to results recorded on a medium containing 2.2 BA and 0.27 NAA (Trejgell et al 2010). By contrast, the multiple rate for shoots stored in glass jars was about half that and differences were statistically significant (Table 1).…”

“…In contrast, during storage of S. macrophyllus shoots, reducing the concentration of MS nutrients, growth regulators and the addition of sorbitol to the medium did not limit the necrotic lesions and slightly inhibited shoot growth during storage. A negative correlation was found between strong growth during storage and viability and regrowth after storage, because a significant reduction in regrowth was observed following storage, in comparison to shoots propagated without cold storage where the rate was more than 8 shoots per explant (Trejgell et al 2010). However, after 6 and 9 months of storage the replacement of 3 % sucrose in the medium on a mixture of 1.5 % sucrose and 1.5 % sorbitol resulted in the highest rate of multiplication among all treatments.…”

“…Shoots were cultured in continuous white fluorescent light (80 lmol m -2 s -1 ) at 26 ± 1°C and subcultured on a 4-week cycle prior to storage (Trejgell et al 2010).…”

An Efficient System for Regenerating Taraxacum Pieninicum Pawł. from Seedling Explants

“…Previous studies on micropropagation of selected Asteraceae species found that cytokinin BA supplementation, either alone or in combination with an auxin, is almost universally effective for in vitro proliferation (Banerjee et al, 2010;Trejgell et al, 2009Trejgell et al, , 2010bTrejgell et al, , and 2012, so in our experiments the medium was supplemented with only BA in combination with NAA at various concentrations. All the tested types of explants showed shoot induction on all the proliferation media used, except for roots on medium with 1 mg L -1 BA (Tab.…”

“…In vitro vegetative propagation of declining (critically endangered, E) and vulnerable (V) species of the Polish flora has been attempted in some dicotyledonous species: for example, Arnica montana (SurmaczMagdziak and Sugier, 2012), Carlina onopordifolia (Trejgell and Tretyn, 2011), Drosera anglica (Kawiak and Łojkowska, 2004), D. intermedia (Kromer et al, 2000), D. rotundifolia (Kawiak et al, 2003), Gentiana pneumonanathe (Bach and Pawłowska, 2003), Leontopodium alpinum (Trejgell et al, 2010a), and Senecio macrophyllus (Trejgell et al, 2010b). The monocotyledons selected for such studies include Fritillaria meleagris (Liliaceae), Gladiolus palustris (Iridaceae) and a few Orchidaceae species (Rybczyński and Mikuła, 2006).…”