Micropropagation of Ginger

Inden, Haruhisa; Asahira, Tadashi; Hirano, Atsushi

doi:10.17660/actahortic.1988.230.20

Cited by 34 publications

(15 citation statements)

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“…Micropropagated ginger is an excellent source of disease-and nematode-free planting material (Hosoki and Sagawa 1977;De Lange et al 1987;Inden et al 1988). However, our study shows that growth and yield of rhizome is inferior to ginger propagated from conventional seed sources.…”

Section: Discussionmentioning

confidence: 61%

“…Micropropagation is an ideal method for mass propagation of pest-and disease-free ginger (Hosoki and Sagawa 1977;De Lange et al 1987;Inden et al 1988); however, little is known about the growth and performance of micropropagated ginger in the field. Recently Bhagyalakshmi et al (1994) compared micropropagated and conventionally propagated ginger in a tropical Indian environment and found lower yields with the micropropagated plants up to 8 months from planting.…”

Section: Introductionmentioning

confidence: 99%

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Field evaluation of micropropagated and conventionally propagated ginger in subtropical Queensland

Smith¹,

Hamill²

1996

Aust. J. Exp. Agric.

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The growth and performance of micropropagated ginger (Zingiber officinale Roscoe) was compared with 'seed'-derived plants in field trials conducted in south-eastern Queensland. In the first generation ex vitro, micropropagated plants had significantly (P<0.01) reduced rhizome yield with smaller knobs and more roots. Micropropagated plants had a greater (P<0.01) shoot: root (rhizome) ratio compared with seed-derived plants. Shoots from micropropagated plants were also significantly (P<0.01) smaller with a greater number of shoots per plant. The unusual shoot morphology of the micropropagated plants did not appear to be related to the presence of benzylaminopurine, a plant growth hormone added to the multiplication medium, as plants subcultured for 3 cycles on a hormone-free medium also exhibited similar characteristics. Seed collected from the micropropagated plants and seed-derived plants was harvested and, despite the micropropagated seed being significantly (P<0.01) smaller, by the second generation ex vitro there were no significant differences between the treatments. Factors that can improve rhizome size, while reducing production costs, need to be identified before micropropagated plants can be recommended for routine use in the ginger industry as a source of disease and pest-free planting material.

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Section: Discussionmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Field evaluation of micropropagated and conventionally propagated ginger in subtropical Queensland

Smith¹,

Hamill²

1996

Aust. J. Exp. Agric.

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“…In vitro multiplication of plant such as ginger (Chang and Chiley, 1993;Hosoki and Sagawa, 1977;Inden et al, 1988), Curcuma amada (Borthakur and Bordoloi, 1992), Alpinia galanga (Borthakur et al, 1999) and Curcuma longa (George, 1993;Misra et al, 2004;Nasirujjaman et al,. 2005;Panda et al,.…”

Section: Methodsmentioning

confidence: 99%

Silver Nitrate and Different Culture Vessels Influence High Frequency Microrhizome Induction In Vitro and Enhancement Growth of Turmeric Plantlet During Ex Vitro Acclimatization

et al. 2012

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Eleven cultivars of C. longa var. Lakadong were collected from Manipur having different topography. Curcumin content in different cultivars has been analyzed by using UV-Visible Spectrophotometer (100 Bio-Carry Spectrophotometer). The curcuminoids content were analyzed and quantified for identification of best quality cultivar. Thoubal Cultivar with highest curcumin content (9.44%) was subjected for tissue culture technique using different culture vessels and silver nitrate for rapid multiplication and scaling up of microrhizome production. High multiplication rate of 27.40±0.47 were obtained in Murashige and Skoog's medium supplemented with 3% sucrose + 1 mg L -1 α-napthalene acetic acid, 4 mg L -1 6-benzyl-amino-purine and 11 µM silver nitrate. Effect of different culture vessels and silver nitrate were studied for microrhizome and multiple shoots formation. Relatively higher rate of shoots along with microrhizome (17.5±0.32) can be seen in Growtek which was grown without any plant growth regulator. Growtek was used for scaling up of microrhizome production in vitro and utmost microrhizome was produced in liquid Murashige and Skoog's medium supplemented with 8% sucrose, 1 mg L -1 α-napthalene acetic acid, 4 mg L -1 6-benzyl-amino-purine and 11 µM silver nitrate (36.25±0.27). Addition of silver nitrate in the medium resulted in improvement of microrhizome induction in vitro. Higher concentration of silver nitrate (33, 44, 66, 88 µM) negatively affected the microrhizome and shoot multiplication and shows inhibition of tissue response completely. Analysis of in vitro derived plantlets during acclimatization shows that the exogenous applied of silver nitrate shows superior growth as compared to control. 90-95% of plantlets with and 75-80% plantlets without silver nitrate treatment were successfully established under ex vitro acclimatization. The protocol could be utilized for large scale production of true-to-type plantlets and as alternative method to step forward towards an improved commercial propagation system for more efficient and productivity.

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“…For ginger, in vitro propagation systems have been reported by various workers, who investigated in vitro systems using different explants types (Hosoki and Sagawa, 1977;De Lange et al, 1987;Bhagyalakshmi, 1988;Inden et al, 1988;Noguchi and Yamakawa, 1988;Ikeda and Tanabe, 1989;Sharma and Singh, 1995). In addition, somatic embryogenesis is frequently regarded as the best system for propagation of superior genotypes (Kim, 2000) mostly because both root and shoot meristems are present simultaneously.…”

Section: Introductionmentioning

confidence: 99%

High Frequency Plant Regeneration System from Transverse Thin Cell Layer Section of <i>In vitro</i> Derived ‘Nadia’ Ginger Microrhizome

Thingbaijam

HUIDROM

2014

Not Sci Biol

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An efficient and reproducible procedure is outlined for rapid in vitro multiplication of Zingiber officinale var. 'Nadia' through high frequency shoot proliferation from transverse thin cell layer (tTCL) sections of in vitro derived microrhizome. In vitro derived microrhizome of size 500 µm in thickness was used as initial explants for induction of somatic embryos. Among the different phytohormones tested, tTCL explants shows maximum calli proliferation in medium containing 2 mg/L 2,4-Dichlorophenoxyacetic acid (88.30±0.11%). Reduced concentration of 2,4-Dichlorophenoxyacetic acid was supplemented with different cytokinins for regeneration of callus. Among the different medium tested, optimum redifferentiation of somatic embryos were observed in medium containing 0.2 mg/L 2,4-Dichlorophenoxyacetic acid and 6.0 mg/L BAP (141.08±0.25). Clump of regenerated plantlets were further subculture and transfer into microrhizome inducing medium containing high sucrose concentration (8%). Plantlets with well developed microrhizome were successfully acclimatized and eventually transferred to the field. The application of studying embryo section for regeneration of plants might be useful alternative to ginger improvement programme. Histological analysis showed formation of somatic embryos and regenerated adventitious shoot.

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Micropropagation of Ginger

Cited by 34 publications

References 0 publications

Field evaluation of micropropagated and conventionally propagated ginger in subtropical Queensland

Field evaluation of micropropagated and conventionally propagated ginger in subtropical Queensland

Silver Nitrate and Different Culture Vessels Influence High Frequency Microrhizome Induction In Vitro and Enhancement Growth of Turmeric Plantlet During Ex Vitro Acclimatization

High Frequency Plant Regeneration System from Transverse Thin Cell Layer Section of <i>In vitro</i> Derived ‘Nadia’ Ginger Microrhizome

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