Micropropagation of ‘Durondeau’ pear in modified-gelled medium

Lucyszyn, Neoli; Quoirin, Marguerite; Ribas, Luciana Lopes Fortes; Koehler, Henrique Soares; Sierakowski, Maria Rita

doi:10.1079/ivp2006753

Cited by 8 publications

(8 citation statements)

References 16 publications

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“…In pear (Pyrus communis L. cv. 'Durondeau'), the addition of guar gum (galactomannans obtained from Cassia fastuosa (cassia) or Cyamopsis tetragonolobus) mixed with agar enhanced the production of non-hyperhydric shoots (Lucyszyn et al, 2006). Addition of certain commercial antihyperhydricity agents ( i.e.…”

Section: Stage I Introduction and Establishmentmentioning

confidence: 99%

Plant tissue culture: Current status, opportunities and challenges

et al. 2010

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R. García-Gonzáles, K. Quiroz, B. Carrasco, and P.D.S. Caligari. 2010. Plant tissue culture: Current status, opportunities and challenges. Cien. Inv. Agr. 37(3): 5-30. In the last two decades plant biotechnology applications have been widely developed and incorporated into the agricultural systems of many countries worldwide. Tissue culture tools have been a key factor to support such outcomes. Current results have allowed plant biotechnology and its products-including transgenic plants with several traits-to be the most assimilated technology for farmers and companies, representing several benefits such as: 125 millions ha of transgenic crops in 2008, the reduction of pesticides application by up to 9% in the last ten years, transgenic plants with a better nutritional quality, mass propagation of selected and healthy plants, and the production of proteins for industrial or therapeutic use. The rapid and extensive assimilation for this technology has improved the competences of the agricultural systems both in industrial and in developing countries, based on the proper application of research programs. Several theoretical and practical aspects supporting plant tissue culture applications, as well as the main results and current status of the technology are discussed in this review. The reader will find key elements to evaluate the potential of plant tissue culture tools for the development of agriculture, livestock, human health and nutrition, and human well being in general.

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Section: Stage I Introduction and Establishmentmentioning

confidence: 99%

Plant tissue culture: Current status, opportunities and challenges

et al. 2010

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“…The galactose distribution at the backbone varies from one botanical source to another and this difference controls properties such as viscosity, solubility, thickening and the stability of solutions 5, 6. For this reason, galactomannans are used as rheology modifiers in food,7, 8 cosmetics,9 pharmaceutical products10 and more recently in biotechnology processes e.g., culturing plant tissue,11–14 among other applications. It can introduce gel properties to aqueous systems, where it is known to participate in synergistic interactions with other polysaccharides such as xanthan,15, 16 carragennan17 and agar 11.…”

Section: Introductionmentioning

confidence: 99%

Dancing with diabetes: brief therapy conversations with children, young people and families living with diabetes

Christie

2008

European Diabetes Nursing

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“…There are a number of different culture media, each with differing salt and vitamin concentrations according to the needs of the species or cultivar. Media are made into gel with different agents (RODRIGUEZ et al, 1991;REED, 2002;LUCYSZYN et al, 2006;BELL et al, 2009), but the most utilized are MS (MURASHIGE;SKOOG, 1962) and its modifications and dilutions, as well as WPM (Wood Plant Medium) (LLOYD; McCOWN, 1980), QL (QUOIRIN;LEPOIVRE, 1977), and DKW (DRIVER; KUNIYUKI, 1984), the formulations of which are considered most diluted.…”

Section: Micropropagationmentioning

confidence: 99%

“…During pear tree multiplication, the source and kind of cytokinin utilized influences the induction and proliferation of axillary buds (HASSANEN;GABR, 2012;KARIMPOUR et al, 2013). Among cytokinins, 6-benzylaminopurine (BAP), is lower cost than many alternatives and is the most utilized for inducing cell division and shoot development (LUCYSZYN et al, 2006;RUZIC et al, 2008;HASSANEN;GABR, 2012).…”

Section: Micropropagationmentioning

confidence: 99%

In vitro multiplication of pear tree cultivar Cascatense

et al. 2016

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One main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) developed and released the cultivar Cascatense, with a low cold requirement (~400 h) better suited for Rio Grande do Sul`s and Santa Catarina`s climate. Still, seedlings of high genetic and sanitary quality are necessary for the establishment of a high-yield orchard. Plants with both sanitary quality and uniformity can be obtained through in vitro micropropagation. Thus, the present study aimed to assess the influence of different culture media (MS, MS¾, WPM, QL, and Himédia), concentrations of sucrose (0, 15, 30, 45, and 60 g L -1 ), and concentrations of benzylaminopurine (BAP) (0, 0.4, 0.8, 1.2, and 1.6 mg L -1 ) upon the in vitro multiplication of cultivar Cascatense, to establish an efficient protocol to enhance its propagation and reproducibility. The study included three sequential experiments in which the number of shoots per explant, the length of shoots, the number of axillary buds per shoot, fresh mass, and dry mass of shoots were evaluated. Cultivar Cascatense can be best propagated in vitro with the use of Himédia medium, supplemented with 0.8 mg L ResumoUm dos principais entraves ao desenvolvimento e expansão da cultura da pereira no Brasil tem sido a falta de cultivares adaptadas às condições edafoclimáticas das regiões potencialmente produtoras. Visando sanar esta dificuldade, a Embrapa Clima Temperado desenvolveu e lançou a cv. Cascatense, com baixa exigência em frio (~400 h), mais adequada ao clima do Rio Grande do Sul e de Santa Catarina. No entanto, mudas com alta qualidade genética e sanitária são necessárias para garantir a boa formação de um pomar produtivo. Plantas com qualidade sanitária e uniformidade podem ser obtidas através da micropropagação in vitro. Assim, o presente trabalho teve como objetivo verificar a influência de diferentes meios de cultivo (MS, MS¾, WPM, QL e Himédia), concentrações de sacarose (0, 15, 30, 45 e 60 g L -1 ) e benzilaminopurina -BAP (0; 0,4; 0,8; 1,2 e 1,6 mg L -1 ) na multiplicação in vitro da cv. Cascatense, visando obter um protocolo eficiente para potencializar sua propagação e reprodutibilidade. Os estudos foram conduzidos em três experimentos sequenciais, nos quais foram avaliados o número de brotações por explante, o comprimento das brotações, o número de gemas axilares por brotação, massa fresca e massa seca das brotações. A cv. Cascatense pode ser propagada in vitro com a utilização do meio Himédia suplementado com 0,8 mg L -1 de BAP e 30 g L -1 de sacarose. Palavras-chave: Meio himédia. Sulfato ferroso. Sulfato férrico. Sacarose. Benzilaminopurina.

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Micropropagation of ‘Durondeau’ pear in modified-gelled medium

Cited by 8 publications

References 16 publications

Plant tissue culture: Current status, opportunities and challenges

Plant tissue culture: Current status, opportunities and challenges

Dancing with diabetes: brief therapy conversations with children, young people and families living with diabetes

In vitro multiplication of pear tree cultivar Cascatense

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