Micropropagation of Dieffenbachia Plants from a Single Stem-Nodes

In Vitro Micropropagation of the Ornamental Plant Dieffenbachia-A Review

“…Axillary branching using axillary bud and stem node, as in other plants, is the majority common explant type utilized for direct shoot propagation of Dieffenbachia. Therefore, shoot induction was reported by many authors on axillary buds [18][19][20][21][22][23][24] and stem nodes [3,22,25]. However, other types of explants were also utilized for micropropagation of Dieffenbachia.…”

“…For example, using even mercuric chloride followed by commercial bleach [39,40], or the highly phytotoxic agent viz. : ethanol followed by commercial bleach [40], chloramine [41] or mercuric chloride [3,40,42]. However, this may indicate that the surface sterilization of Dieffenbachia explant depends on the cultivar used and mother plant more than explant nature.…”

“…El-Sawy and Bakheet [26], Feng et al [20], Hui et al [37], Iqbal et al [29], Mogollon [27], Jun et al [25] El-Mahrouk et al [14], El-Mahrouk et al [42], Shen and Lee, [23] Elsheikh and Khalfalla [24] and Abass et al, [44] employed full MS for bud proliferation, callus induction and rapid multiplication of Dieffenbachia. However, some modifications on MS medium were stated to improve multiplication such as Shen and Lee [34] which used full MS with 2% sucrose and 1% glucose, El-Mahrouk et al [3] and Chao and Li-si [22] exploited half-strength MS medium and Shen and Lee [35] as well as added 2% glucose. However, previously, Taylor and Knauss [19] developed DM basic medium for tissue culture of Dieffenbachia which is identical to MS medium except for the addition of adenine sulphate 80 mg/L, and NaH2PO4.H2O 170 mg/L.…”

“…Litz and Conover [18] employed 8.0 mg/L BA for rapid in vitro propagation of Dieffenbachia sp. El-Mahrouk et al [3] fortified MS media with 8.0 mg/L BA and 0.1 mg/L NAA to multiply stem nodes of D. amoena cv. Tropic snow.…”

“…The genus Dieffenbachia consists of about 30 species and over 100 cultivars with spotted, striped or speckled with cream, white, yellow, gold, silver, or a combination of these colors leaves, 15 to 40 cm or so in length [1,2]. Dieffenbachia is an ornamental perennial monocot plant native to tropical America [3], belonging to the family Araceae [4]. It is prized among interiorscapers for the attractive variegated foliage, tolerance of interior environments and easy production [5].…”