Micropropagation of Dendrobium nobile from shoot tip sections

Malabadi, Ravindra B.; Mulgund, Gangadhar S.; Kallappa, Nataraja

doi:10.1016/j.jplph.2004.09.008

Cited by 61 publications

(40 citation statements)

References 18 publications

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“…Tissue culture techniques are ultimately an effective solution for the mass multiplication of D. nobile in a short time span (Malabadi et al, 2005). Nayak et al (2002) used zeatin riboside (ZR), N 6 -benzyladenine (BA) and kinetin (Kin) to induce shoot proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…Nayak et al (2002) used zeatin riboside (ZR), N 6 -benzyladenine (BA) and kinetin (Kin) to induce shoot proliferation. Similarly, Malabadi et al (2005) presented an idea for mass multiplication of D. nobile by using different concentrations (11 to 15 µM g/l) of triacontanol. Aktar et al (2008) supplemented ½ MS and KC medium with banana pulp to compare their effects on the growth of D. nobile.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro propagation of orchid (Dendrobium nobile) var. Emma white

Sana¹,

Ahmad²,

Ahmad³

et al. 2011

Afr. J. Biotechnol.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro propagation of orchid (Dendrobium nobile) var. Emma white

Sana¹,

Ahmad²,

Ahmad³

et al. 2011

Afr. J. Biotechnol.

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show abstract

“…The need for mass propagation has led to the development of in vitro methods such as the propagation of large-scale PLBs using: shoot tips (Malabadi et al, 2005;Sheela et al, 2004), leaf segments (Martin and Madassery, 2006;Park et al, 2002a), protocorms (Sheelavanthmath et al, 2005;Teng et al, 2004), flower stalks (Chen and Chang, 2000;Chen et al, 2002), stem segments (Luo et al, 2008) and root tips (Manners et al, 2010;Park et al, 2003). PLBs can proliferate rapidly and can readily regenerate into complete plantlets, so they are also the most general target tissue for genetic transformation studies in orchids (Liau et al, 2003;Sreeramanan et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Efficient Direct Protocorm-Like Bodies Induction of Dwarf Dendrobium using Thidiazuron

Sujjaritthurakarn

Kanchanapoom

2011

Not Sci Biol

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An efficient induction of protocorm-like bodies (PLBs) from seedlings of dwarf Dendrobium was accomplished in order to develop mass-scale propagation. The effect of N 6 -benzyladenine (BA) and thidiazuron (TDZ) on the in vitro induction of PLBs was studied. TDZ was found to be a more effective inducer of PLBs and their proliferation than BA. The highest percentage for the induction of PLBs (86%) and the highest number of PLBs (3.6) per protocorm were observed after 9 weeks of culture in modified Murashige and Skoog (MS) liquid medium supplemented with 18 μM TDZ.

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“…Micropropagation technologies must be used to cultivate this plant to ensure its continued availability and economic value. Many micropropagation protocols have been developed using axenic nodal segments of shoot, shoot tip, axillary shoot, foliar explants and PLBs (Martin and Joseph 2006;Shiau et al 2005;Malabadi et al 2005;Luo et al 2008;Yan et al 2010), and PLBs as explants have emerged as an effective and rapid technique (Nayak et al 2002;Santos Díaz and Carranza Á lvarez 2009). Exogenous applications of spermidine and putrescine have been used to promote shooting of Dendrobium PLBs (Wang et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro

Lin

et al. 2010

Plant Cell Tiss Organ Cult

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The effect of light quality on protocorm-like bodies (PLBs) of Dendrobium officinale was investigated. PLBs of D. officinale were incubated under a number of different light conditions in vitro, namely: dark conditions; fluorescent white light (Fw); red light-emitting diodes (LEDs); blue LEDs; half red plus half blue [RB (1:1)] LEDs; 67% red plus 33% blue [RB (2:1)] LEDs; and 33% red plus 67% blue [RB (1:2)] LEDs. Growth parameters, number of shoots produced per PLB, chlorophyll concentration and carotenoid concentration were measured after 90 days culture. The percentage of PLBs producing shoots was 85% under blue LEDs. In contrast, the percentage of PLBs producing shoots was less than 60% under dark conditions, fluorescent white light and red LEDs. The number of shoots produced per PLB was more than 1.5 times greater under blue LEDs, RB (1:1) LEDs and RB (1:2) LEDs than those cultured under other light treatments [dark, Fw, red LEDs and RB (2:1)]. Chlorophyll and carotenoid concentrations were significantly higher under blue LEDs and different red plus blue LED ratios, compared to other light treatments (dark, Fw and red LEDs). Blue LEDs, Fw, and RB (1:2) LEDs produced higher dry matter accumulations of PLBs and shoots. This study suggests that blue LEDs or RB (1:2) LEDs could significantly promote the production of shoots by protocorm-like bodies of D. officinale and increase the dry matter of PLBs and the accumulation of shoot dry matter in vitro.

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Micropropagation of Dendrobium nobile from shoot tip sections

Cited by 61 publications

References 18 publications

In vitro propagation of orchid (Dendrobium nobile) var. Emma white

In vitro propagation of orchid (Dendrobium nobile) var. Emma white

Efficient Direct Protocorm-Like Bodies Induction of Dwarf Dendrobium using Thidiazuron

Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro

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